An Innovative Test for the Rapid Detection of Specific IgG Antibodies in Human Whole-Blood for the Diagnosis of Opisthorchis viverrini Infection
Abstract
:1. Introduction
2. Materials and Methods
2.1. Parasite and Antigen Preparations
2.2. Preparation of Simulated WBS
2.3. Clinical Samples
2.4. Preparation of the Immunochromatographic Test
2.5. Immunochromatographic Testing Method
2.6. Data Analysis
3. Results
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Type of Samples | Number of Positive/Total Samples Evaluated (Intensity Level of Bands a) |
---|---|
Healthy volunteers | 0/40 |
Opisthorchiasis | 107/112 (0.5–5) |
Lecithodendriid fluke infections | 4/10 (1) |
Clonorchiasis | 30/35 (0.5–4) |
Fascioliasis | 7/10 (0.5–3) |
Paragonimiasis | 6/10 (0.5–2) |
Cysticercosis | 1/10 (0.5) |
Taeniasis saginata | 0/10 |
Sparganosis | 1/10 (0.5) |
Ascariasis | 0/10 |
Angiostrongyliasis | 1/10 (1) |
Hookworm infections | 1/10 (0.5) |
Trichinellosis | 3/10 (0.5–1) |
Trichuriasis | 0/10 |
Capillariasis philippinensis | 0/10 |
Gnathostomiasis | 0/10 |
Strongyloidiasis | 0/10 |
Blastocystosis | 1/10 (2) |
Giardiasis | 1/10 (2) |
Total number of evaluated samples | 347 |
Data | Opisthorchiasis (Ov) a | Clonorchiasis (Cs) b | Ov and Cs c |
---|---|---|---|
Sensitivity (%) | 95.5 (89.9–98.5) | 85.7 (69.7–95.2) | 93.2 (87.8–96.7) |
Specificity (%) | 87.0 (81.5–91.3) | 87.0 (81.5–91.3) | 87.0 (81.5–91.3) |
Positive predictive value (%) | 80.5 (72.7–86.8) | 53.6 (39.7–67.0) | 84.0 (77.5–89.3) |
Negative predictive value (%) | 97.2 (93.6–99.1) | 97.2 (93.6–99.1) | 94.6 (90.2–97.4) |
Positive likelihood ratio | 7.35 (5.12– 10.5) | 6.59 (4.49–9.67) | 7.17 (5.0–10.3) |
Negative likelihood ratio | 0.05 (0.02–0.12) | 0.16 (0.07–0.37) | 0.10 (0.04–0.14) |
Accuracy (%) | 90.1 (86.2–93.2) | 86.8 (81.8–90.9) | 89.6 (85.9–92.6) |
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Sadaow, L.; Rodpai, R.; Janwan, P.; Boonroumkaew, P.; Sanpool, O.; Thanchomnang, T.; Yamasaki, H.; Ittiprasert, W.; Mann, V.H.; Brindley, P.J.; et al. An Innovative Test for the Rapid Detection of Specific IgG Antibodies in Human Whole-Blood for the Diagnosis of Opisthorchis viverrini Infection. Trop. Med. Infect. Dis. 2022, 7, 308. https://doi.org/10.3390/tropicalmed7100308
Sadaow L, Rodpai R, Janwan P, Boonroumkaew P, Sanpool O, Thanchomnang T, Yamasaki H, Ittiprasert W, Mann VH, Brindley PJ, et al. An Innovative Test for the Rapid Detection of Specific IgG Antibodies in Human Whole-Blood for the Diagnosis of Opisthorchis viverrini Infection. Tropical Medicine and Infectious Disease. 2022; 7(10):308. https://doi.org/10.3390/tropicalmed7100308
Chicago/Turabian StyleSadaow, Lakkhana, Rutchanee Rodpai, Penchom Janwan, Patcharaporn Boonroumkaew, Oranuch Sanpool, Tongjit Thanchomnang, Hiroshi Yamasaki, Wannaporn Ittiprasert, Victoria H. Mann, Paul J. Brindley, and et al. 2022. "An Innovative Test for the Rapid Detection of Specific IgG Antibodies in Human Whole-Blood for the Diagnosis of Opisthorchis viverrini Infection" Tropical Medicine and Infectious Disease 7, no. 10: 308. https://doi.org/10.3390/tropicalmed7100308