Comparative Analysis of a Rapid Quantitative Immunoassay to the Reference Methodology for the Measurement of Blood Vitamin D Levels
Abstract
1. Introduction
2. Materials and Methods
2.1. Study Volunteers
2.2. Capillary Blood Vitamin D Test—Rapi-D and IgLoo Reader
2.3. Dried Blood Spot Vitamin D Testing—Laboratory Reference Method
2.4. Data Analysis and Statistics
3. Results
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Total Number of Participants, n | 48 |
---|---|
Males (%) | 21 (44) |
Females (%) | 27 (56) |
Mean age in years (SD) | 34.2 (8.99) |
Vitamin D supplementation: number of volunteers that report taking dietary supplements within the last month prior to sampling (%) | 24 (48) |
Ethnicity, self-reported, n (%) | Asian 19 (40) |
Black 12 (25) | |
White 13 (27) | |
Hispanic 1 (2) | |
Arab 1 (2) | |
Mixed 2 (4) | |
Mean body mass index kg/m2 (SD) | 24.38 (4.57) |
Mean vitamin D status nmol/L * | |
Rapid test with IgLoo reader (SD) | 56.2 (27.4) |
DBS and LC/MS-MS (SD) | 37.4 (21.4) |
Subject ID | IgLoo Vitamin D Levels from Reader (ng/mL) | IgLoo Vitamin D Levels (nmol/L) | IgLoo Vitamin D Levels Interpretation a | DBS Vitamin D Levels (nmol/L) | DBS Vitamin D levels Interpretation a |
---|---|---|---|---|---|
0001 | 12.4 | 31 | Insufficient | 34.65 | Insufficient |
0002 | 36.2 | 90.5 | Sufficient | 65.7 | Sufficient |
0003 | 7.86 | 19.65 | Deficient | 12.1 | Deficient |
0004 | 17.5 | 43.75 | Insufficient | 25.2 | Insufficient |
0005 | 20.3 | 50.75 | Sufficient | 31.3 | Insufficient |
0006 | 7.32 | 18.3 | Deficient | 7.65 | Deficient |
0007 | 24.3 | 60.75 | Sufficient | 45.7 | Insufficient |
0008 | 39.2 | 98 | Sufficient | 47.5 | Insufficient |
0009 | 35.8 | 89.5 | Sufficient | 59.5 | Sufficient |
0010 | 16.3 | 40.75 | Insufficient | 25.25 | Insufficient |
0011 | 18 | 45 | Insufficient | 27.35 | Insufficient |
0012 | 26.7 | 66.75 | Sufficient | 47.45 | Insufficient |
0013 | 8.18 | 20.45 | Deficient | 7.15 | Deficient |
0014 | 31.2 | 78 | Sufficient | 50.35 | Sufficient |
0015 | 25.4 | 63.5 | Sufficient | 50.5 | Sufficient |
0016 | 36.7 | 91.75 | Sufficient | 62.85 | Sufficient |
0017 | 5.73 | 14.325 | Deficient | 16.8 | Deficient |
0018 | 10.7 | 26.75 | Insufficient | 17.85 | Deficient |
0019 | 21.7 | 54.25 | Sufficient | 30.4 | Insufficient |
0020 | 15.1 | 37.75 | Insufficient | 34.5 | Insufficient |
0021 | 23.3 | 58.25 | Sufficient | 50.3 | Sufficient |
0022 | 32.6 | 81.5 | Sufficient | 70.85 | Sufficient |
0023 | 21.1 | 52.75 | Sufficient | 26 | Insufficient |
0024 | 5.11 | 12.775 | Deficient | 7.5 | Deficient |
0025 | 14.1 | 35.25 | Insufficient | 21 | Deficient |
0026 | 15.4 | 38.5 | Insufficient | 30.5 | Insufficient |
0027 | 12 | 30 | Insufficient | 23.15 | Deficient |
0028 | 38.3 | 95.75 | Sufficient | 60.55 | Sufficient |
0029 | 46.6 | 116.5 | Sufficient | 59.5 | Sufficient |
0030 | 17.7 | 44.25 | Insufficient | 26.95 | Insufficient |
0031 | 12.7 | 31.75 | Insufficient | 18.35 | Deficient |
0032 | 27.8 | 69.5 | Sufficient | 47.35 | Insufficient |
0033 | 24.6 | 61.5 | Sufficient | 29.3 | Insufficient |
0034 | 15.6 | 39 | Insufficient | 22.5 | Deficient |
0035 | >150 | n.d. | High b | 80 | Sufficient |
0036 | 28.3 | 70.75 | Sufficient | 49.05 | Insufficient |
0037 | 26.1 | 65.25 | Sufficient | 31.45 | Insufficient |
0038 | 34.7 | 86.75 | Sufficient | 54.7 | Sufficient |
0039 | 43.4 | 108.5 | Sufficient | 119.1 | Sufficient |
0040 | 30.1 | 75.25 | Sufficient | 54.45 | Sufficient |
0041 | 20.4 | 51 | Sufficient | 32.8 | Insufficient |
0042 | 46.1 | 115.25 | Sufficient | 57.15 | Sufficient |
0043 | 24.8 | 62 | Sufficient | n.m. | n.m. |
0044 | 15.9 | 39.75 | Insufficient | 15.8 | Deficient |
0045 | 14 | 35 | Insufficient | 12.8 | Deficient |
0046 | 20 | 50 | Sufficient | 26.35 | Insufficient |
0047 | 27.3 | 68.25 | Sufficient | 52.55 | Sufficient |
0048 | 26.5 | 66.25 | Sufficient | 56.9 | Sufficient |
0049 | 16.7 | 41.75 | Insufficient | 28.9 | Insufficient |
0050 | 6.05 | 15.125 | Deficient | 8.25 | Deficient |
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McLean, G.R.; Soyemi, S.; Ajayi, O.P.; Fernando, S.; Sowinski-Mydlarz, W.; Stewart, D.; Illingworth, S.; Atkins, M.; Bhakta, D. Comparative Analysis of a Rapid Quantitative Immunoassay to the Reference Methodology for the Measurement of Blood Vitamin D Levels. Methods Protoc. 2025, 8, 85. https://doi.org/10.3390/mps8040085
McLean GR, Soyemi S, Ajayi OP, Fernando S, Sowinski-Mydlarz W, Stewart D, Illingworth S, Atkins M, Bhakta D. Comparative Analysis of a Rapid Quantitative Immunoassay to the Reference Methodology for the Measurement of Blood Vitamin D Levels. Methods and Protocols. 2025; 8(4):85. https://doi.org/10.3390/mps8040085
Chicago/Turabian StyleMcLean, Gary R., Samson Soyemi, Oluwafunmito P. Ajayi, Sandra Fernando, Wiktor Sowinski-Mydlarz, Duncan Stewart, Sarah Illingworth, Matthew Atkins, and Dee Bhakta. 2025. "Comparative Analysis of a Rapid Quantitative Immunoassay to the Reference Methodology for the Measurement of Blood Vitamin D Levels" Methods and Protocols 8, no. 4: 85. https://doi.org/10.3390/mps8040085
APA StyleMcLean, G. R., Soyemi, S., Ajayi, O. P., Fernando, S., Sowinski-Mydlarz, W., Stewart, D., Illingworth, S., Atkins, M., & Bhakta, D. (2025). Comparative Analysis of a Rapid Quantitative Immunoassay to the Reference Methodology for the Measurement of Blood Vitamin D Levels. Methods and Protocols, 8(4), 85. https://doi.org/10.3390/mps8040085