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Impedance Measures for Detecting Electrical Responses during Acute Injury and Exposure of Compounds to Roots of Plants
 
 
Protocol

Measuring Electrical Responses during Acute Exposure of Roots and Rhizoids of Plants to Compounds Using a Flow-Through System

1
Department of Biology, University of Kentucky, Lexington, KY 40506-0225, USA
2
Department of Biology, Eastern Kentucky University, Richmond, KY 40475-3102, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Lee Hansen and Jongik Hwang
Methods Protoc. 2022, 5(4), 62; https://doi.org/10.3390/mps5040062
Received: 29 April 2022 / Revised: 1 July 2022 / Accepted: 14 July 2022 / Published: 18 July 2022
(This article belongs to the Special Issue Methods and Protocols 2022)
Monitoring electrical signals in plants allows the examination of their acute and chronic physiological changes and responses to stimuli. Understanding how plant roots/rhizoids respond to chemical cues in their environment will provide insight into how these structures acquire resources. Chronic exposure to L-glutamate alters root growth and is known to alter Ca2+ flux inside roots. The ionic flux can be detected by electrical changes. A rapid and relatively easy approach is presented to screen the electrical sensitivity of roots/rhizoids to compounds such as amino acids and known agonists/antagonists to receptors and ion channels. The approach uses a background-flow system of basal salt or water; then, the administered compounds are added to the roots/rhizoids while monitoring their electrical responses. As a proof of concept, the response to flow-through of glutamate (1 mM) was targeted at the root/rhizoids of three plants (Arabidopsis thaliana, Pisum sativum and Marchantia inflexa). Both Arabidopsis thaliana and Pisum sativum produced rapid depolarization upon exposure to glutamate, while M. inflexa did not show an electrical response. In some experiments, simultaneous recordings with impedance measures for acute changes and glass electrodes for chronic electrical potential changes were used. The effect of potassium chloride (300 mM) as a depolarizing stimulus produced responses in both P. sativum and M. inflexa. The protocol presented can be used to screen various compounds in a relatively rapid manner for responsiveness by the roots/rhizoids of plants. View Full-Text
Keywords: roots; electrophysiology; flow-though roots; electrophysiology; flow-though
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Figure 1

  • Externally hosted supplementary file 1
    Link: https://www.youtube.com/watch?v=3151I9q0WUc
    Description: The following supporting information can be downloaded at: www.mdpi.com/xxx/s1, Video S1: Procedure to record from Arabidopsis thaliana.https://www.youtube.com/watch?v=3151I9q0WUc
  • Externally hosted supplementary file 2
    Link: https://www.youtube.com/watch?v=6J6G70iW2pM
    Description: The following supporting information can be downloaded at: www.mdpi.com/xxx/s1, Video S2: Proce-dure to record from Pisum sativum. https://www.youtube.com/watch?v=6J6G70iW2pM
  • Externally hosted supplementary file 3
    Link: https://www.youtube.com/watch?v=dK-xxF2iZj8
    Description: The following supporting information can be downloaded at: www.mdpi.com/xxx/s1, Video S3: Procedure to record from Marchantia inflexa. https://www.youtube.com/watch?v=dK-xxF2iZj8
MDPI and ACS Style

Cooper, R.L.; Thomas, M.A.; Vascassenno, R.M.; Brock, K.E.; McLetchie, D.N. Measuring Electrical Responses during Acute Exposure of Roots and Rhizoids of Plants to Compounds Using a Flow-Through System. Methods Protoc. 2022, 5, 62. https://doi.org/10.3390/mps5040062

AMA Style

Cooper RL, Thomas MA, Vascassenno RM, Brock KE, McLetchie DN. Measuring Electrical Responses during Acute Exposure of Roots and Rhizoids of Plants to Compounds Using a Flow-Through System. Methods and Protocols. 2022; 5(4):62. https://doi.org/10.3390/mps5040062

Chicago/Turabian Style

Cooper, Robin Lewis, Matthew A. Thomas, Rachael M. Vascassenno, Kaitlyn E. Brock, and David Nicholas McLetchie. 2022. "Measuring Electrical Responses during Acute Exposure of Roots and Rhizoids of Plants to Compounds Using a Flow-Through System" Methods and Protocols 5, no. 4: 62. https://doi.org/10.3390/mps5040062

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