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Open AccessProtocol

Fibrin Clot Formation and Lysis in Plasma

1
Thrombosis and Haemostasis Research Unit, Department of Clinical Biochemistry, Aarhus University Hospital, Palle Juul-Jensens Boulevard 99, 8200 Aarhus, Denmark
2
Department of Clinical Medicine, Health, Aarhus University, Palle Juul-Jensens Boulevard 82, 8200 Aarhus, Denmark
*
Author to whom correspondence should be addressed.
Methods Protoc. 2020, 3(4), 67; https://doi.org/10.3390/mps3040067
Received: 7 September 2020 / Revised: 21 September 2020 / Accepted: 23 September 2020 / Published: 25 September 2020
(This article belongs to the Special Issue Feature Issue on the Development of Novel Cutting Edge Protocols)
Disturbance in the balance between fibrin formation and fibrinolysis can lead to either bleeding or thrombosis; however, our current routine coagulation assays are not sensitive to altered fibrinolysis. The clot formation and lysis assay is a dynamic plasma-based analysis that assesses the patient’s capacity for fibrin formation and fibrinolysis by adding an activator of coagulation as well as fibrinolysis to plasma and measuring ex vivo fibrin clot formation and breakdown over time. This assay provides detailed information on the fibrinolytic activity but is currently used for research only, as the assay is prone to inter-laboratory variation and as it demands experienced laboratory technicians as well as specialized personnel to validate and interpret the results. Here, we describe a protocol for the clot formation and lysis assay used at our research laboratory. View Full-Text
Keywords: blood coagulation; fibrinolysis; clot formation and lysis assay blood coagulation; fibrinolysis; clot formation and lysis assay
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MDPI and ACS Style

Larsen, J.B.; Hvas, A.-M. Fibrin Clot Formation and Lysis in Plasma. Methods Protoc. 2020, 3, 67. https://doi.org/10.3390/mps3040067

AMA Style

Larsen JB, Hvas A-M. Fibrin Clot Formation and Lysis in Plasma. Methods and Protocols. 2020; 3(4):67. https://doi.org/10.3390/mps3040067

Chicago/Turabian Style

Larsen, Julie B.; Hvas, Anne-Mette. 2020. "Fibrin Clot Formation and Lysis in Plasma" Methods Protoc. 3, no. 4: 67. https://doi.org/10.3390/mps3040067

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