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Article

The Making of Transgenic Drosophila guttifera

1
Department of Biological Sciences, Michigan Technological University, Houghton, MI 49931, USA
2
Faculty of Environmental Earth Science, Hokkaido University, N10W5, Kita-ku, Sapporo, Hokkaido 060-0810, Japan
3
Graduate School of Environmental Science, Hokkaido University, N10W5, Kita-ku, Sapporo, Hokkaido 060-0810, Japan
*
Authors to whom correspondence should be addressed.
Methods Protoc. 2020, 3(2), 31; https://doi.org/10.3390/mps3020031
Received: 7 April 2020 / Revised: 25 April 2020 / Accepted: 26 April 2020 / Published: 27 April 2020
The complex color patterns on the wings and body of Drosophila guttifera (D. guttifera) are emerging as model systems for studying evolutionary and developmental processes. Studies regarding these processes depend on overexpression and downregulation of developmental genes, which ultimately rely upon an effective transgenic system. Methods describing transgenesis in Drosophila melanogaster (D. melanogaster) have been reported in several studies, but they cannot be applied to D. guttifera due to the low egg production rate and the delicacy of the eggs. In this protocol, we describe extensively a comprehensive method used for generating transgenic D. guttifera. Using the protocol described here, we are able to establish transgenic lines, identifiable by the expression of enhanced green fluorescent protein (EGFP) in the eye disks of D. guttifera larvae. The entire procedure, from injection to screening for transgenic larvae, can be completed in approximately 30 days and should be relatively easy to adapt to other non-model Drosophila species, for which no white-eyed mutants exist.
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Keywords: microinjection; Drosophila guttifera; transgenesis; piggyBac microinjection; Drosophila guttifera; transgenesis; piggyBac
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MDPI and ACS Style

Shittu, M.; Steenwinkel, T.; Koshikawa, S.; Werner, T. The Making of Transgenic Drosophila guttifera. Methods Protoc. 2020, 3, 31. https://doi.org/10.3390/mps3020031

AMA Style

Shittu M, Steenwinkel T, Koshikawa S, Werner T. The Making of Transgenic Drosophila guttifera. Methods and Protocols. 2020; 3(2):31. https://doi.org/10.3390/mps3020031

Chicago/Turabian Style

Shittu, Mujeeb, Tessa Steenwinkel, Shigeyuki Koshikawa, and Thomas Werner. 2020. "The Making of Transgenic Drosophila guttifera" Methods and Protocols 3, no. 2: 31. https://doi.org/10.3390/mps3020031

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