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Characterizing the Anoxic Phenotype of Pseudomonas putida Using a Bioelectrochemical System

1
Systems Biotechnology group, Department of Solar Materials, Helmholtz Centre for Environmental Research—UFZ, 04318 Leipzig, Germany
2
Center for Microbial Electrochemical Systems (CEMES), Advanced Water Management Center, The University of Queensland, Brisbane QLD 4072, Australia
*
Author to whom correspondence should be addressed.
Methods Protoc. 2019, 2(2), 26; https://doi.org/10.3390/mps2020026
Received: 11 March 2019 / Revised: 26 March 2019 / Accepted: 27 March 2019 / Published: 30 March 2019
Industrial fermentation in aerobic processes is plagued by high costs due to gas transfer limitations and substrate oxidation to CO2. It has been a longstanding challenge to engineer an obligate aerobe organism, such as Pseudomonas putida, into an anaerobe to facilitate its industrial application. However, the progress in this field is limited, due to the poor understanding of the constraints restricting its anoxic phenotype. In this paper, we provide a methodological description of a novel cultivation technology for P. putida under anaerobic conditions, using the so-called microbial electrochemical technology within a bioelectrochemical system. By using an electrode as the terminal electron acceptor (mediated via redox chemicals), glucose catabolism could be activated without oxygen present. This (i) provides an anoxic-producing platform for sugar acid production at high yield and (ii) more importantly, enables systematic and quantitative characterizations of the phenotype of P. putida in the absence of molecular oxygen. This unique electrode-based cultivation approach offers a tool to understand and in turn engineer the anoxic phenotype of P. putida and possibly also other obligate aerobes. View Full-Text
Keywords: Pseudomonas putida; Microbial electrochemical technology; bioelectrochemical system; anoxic phenotype; anaerobic cultivation Pseudomonas putida; Microbial electrochemical technology; bioelectrochemical system; anoxic phenotype; anaerobic cultivation
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Lai, B.; Nguyen, A.V.; Krömer, J.O. Characterizing the Anoxic Phenotype of Pseudomonas putida Using a Bioelectrochemical System. Methods Protoc. 2019, 2, 26.

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