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Methods Protoc. 2019, 2(1), 5; https://doi.org/10.3390/mps2010005

A Novelty System for Biotization of Plant Microshoots and Collection of Natural Compounds

1
Institute of Mediterranean Agricultural and Environmental Sciences (ICAAM). Colégio da Mitra, Ap. 94, 7002-554 Évora, Portugal
2
Banco de GermoplasmaISOPlexis, Campus da Penteada, Universidade da Madeira, 9020-105 Funchal, Portugal
3
Departamento de Biologia, Pólo da Mitra Apartado 94, 7002-554 Évora, Portugal
4
Research Centre in Biodiversity and Genetic Resources Évora (CIBIO-UE), Ap. 94, 7002-554 Évora, Portugal
5
Instituto de Ciências da Terra (ICT), Colégio Luís António Verney, Rua Romão Ramalho 59, 7000-671 Évora, Portugal
*
Author to whom correspondence should be addressed.
Received: 23 October 2018 / Revised: 27 December 2018 / Accepted: 30 December 2018 / Published: 7 January 2019
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Abstract

An in vitro plant microshoot culture system composed of two phases; a liquid phase overlaid by a floating solid phase, which is described in detail herein. This system is designed to enable the extraction of natural compounds released/disseminated into the liquid phase during root growth, thus facilitating their processing and biochemical characterization. The solid phase holds the plant afloat and enables the simultaneous culture of a microorganism, yet avoiding its penetration into the liquid phase, where the roots are submerged. Both phases can be independently formulated as required for growth optimization of both organisms. Considering the closed system and known variables described in this patent, applications of the described method include testing with pesticides, herbicides, and other similar products. View Full-Text
Keywords: Keywords: biotechnology; biotization; chemical analysis; double-phase medium; phenolic compounds; liquid chromatography—diode array detector—mass spectrometry Keywords: biotechnology; biotization; chemical analysis; double-phase medium; phenolic compounds; liquid chromatography—diode array detector—mass spectrometry
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Castro, M.R.C.B.; Ragonezi, C.; Oliveira, P.G.L.; Zavattieri, M.A. A Novelty System for Biotization of Plant Microshoots and Collection of Natural Compounds. Methods Protoc. 2019, 2, 5.

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