A Novelty System for Biotization of Plant Microshoots and Collection of Natural Compounds
AbstractAn in vitro plant microshoot culture system composed of two phases; a liquid phase overlaid by a floating solid phase, which is described in detail herein. This system is designed to enable the extraction of natural compounds released/disseminated into the liquid phase during root growth, thus facilitating their processing and biochemical characterization. The solid phase holds the plant afloat and enables the simultaneous culture of a microorganism, yet avoiding its penetration into the liquid phase, where the roots are submerged. Both phases can be independently formulated as required for growth optimization of both organisms. Considering the closed system and known variables described in this patent, applications of the described method include testing with pesticides, herbicides, and other similar products. View Full-Text
Share & Cite This Article
Castro, M.R.C.B.; Ragonezi, C.; Oliveira, P.G.L.; Zavattieri, M.A. A Novelty System for Biotization of Plant Microshoots and Collection of Natural Compounds. Methods Protoc. 2019, 2, 5.
Castro MRCB, Ragonezi C, Oliveira PGL, Zavattieri MA. A Novelty System for Biotization of Plant Microshoots and Collection of Natural Compounds. Methods and Protocols. 2019; 2(1):5.Chicago/Turabian Style
Castro, Mário R.C.B.; Ragonezi, Carla; Oliveira, Paulo G.L.; Zavattieri, Maria A. 2019. "A Novelty System for Biotization of Plant Microshoots and Collection of Natural Compounds." Methods Protoc. 2, no. 1: 5.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.