DNA Extraction with DNAzol and LAMP, Performed in a Heating Block as a Simple Procedure for Detection of Mycobacterium tuberculosis in Sputum Specimens
Facultad de Ciencias Químicas e Ingeniería, Universidad Autónoma de Baja California, Calzada Universidad 14418, Tijuana, BCN 22390, Mexico
Laboratorio de Diagnóstico Clínico, Hospital General de Tijuana, Vía de la Juventud Oriente 4910, Tijuana, BCN 22010, Mexico
Departamento de Innovación Biomédica, Centro de Investigación Científica y Educación Superior de Ensenada, Carretera Ensenada-Tijuana 3918, Ensenada, BCN 22860, Mexico
Author to whom correspondence should be addressed.
Methods Protoc. 2018, 1(4), 37; https://doi.org/10.3390/mps1040037
Received: 25 September 2018 / Revised: 16 October 2018 / Accepted: 19 October 2018 / Published: 23 October 2018
Tuberculosis (TB) remains as a major public health issue in developing countries. Accurate detection is essential for the proper management of patients with active disease. Here, we present a simple DNAzol-LAMP (loop-mediated isothermal amplification) procedure for the detection of Mycobacterium tuberculosis in sputum specimens. Twenty smear-positive sputum samples were analyzed as follows: (i) Genetic material was extracted by a standard DNAzol protocol, and (ii) mycobacterial DNA was detected by a typical TB-specific loop-mediated isothermal amplification method. Results and diagnostic test performance attests to the suitability of the proposed procedure.