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Influence of Hematocrit and Total-Spot Volume on Performance Characteristics of Dried Blood Spots for Newborn Screening

Newborn Screening and Molecular Biology Branch, Division of Laboratory Sciences, National Center for Environmental Health, US Centers for Disease Control and Prevention, 4770 Buford Highway, NE, Mail Stop F-19, Atlanta, GA 30341, USA
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Academic Editor: Ralph Fingerhut
Int. J. Neonatal Screen. 2015, 1(2), 69-78; https://doi.org/10.3390/ijns1020069
Received: 27 May 2015 / Revised: 6 August 2015 / Accepted: 17 August 2015 / Published: 21 August 2015
Dried blood spots (DBS) have been used in newborn screening (NBS) tests for over 50 years. The Newborn Screening Quality Assurance Program (NSQAP) at the Centers for Disease Control and Prevention (CDC) conducted studies to assess the individual impacts of hematocrit and total-spot volume on characteristics of DBS samples. Per-punch serum volumes decreased 27%, RBC volumes more than doubled, absorption times increased over 300%, and spot diameters decreased marginally between the hematocrits of 40% to 65%. Per-punch serum and RBC volumes decreased logarithmically with lowering total-spot volumes. Patient hematocrit is an uncontrollable variable and inevitably affects the resulting punch from a DBS sample. It may be possible, though, to identify samples that fall outside of an acceptable range by noting certain physical characteristics of the DBS. View Full-Text
Keywords: dried blood spots; newborn screening; hematocrit; filter paper; specimen collection device dried blood spots; newborn screening; hematocrit; filter paper; specimen collection device
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Hall, E.M.; Flores, S.R.; De Jesús, V.R. Influence of Hematocrit and Total-Spot Volume on Performance Characteristics of Dried Blood Spots for Newborn Screening. Int. J. Neonatal Screen. 2015, 1, 69-78.

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Int. J. Neonatal Screen., EISSN 2409-515X, Published by MDPI AG
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