Potential Uses of Scallop Shell Powder as a Substrate for the Cultivation of King Oyster Mushroom (Pleurotus eryngii)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Inoculum Source and Spawn Preparation
2.2. Preparation of Ca Powder (SSP)
2.3. Substrate Preparation
2.4. Assay for the Growth Rate
2.5. Mushroom Cultivation
2.6. Composition Analysis
2.7. Composition Statistics
3. Results
3.1. Growth Rate of Mycelia
3.2. Characteristics of P. eryngii Fruiting Bodies on Different Substrates
3.3. Nutrient Content of Mushrooms
3.4. Macronutrient Elements of Mushrooms
3.5. Micronutrient Elements of Mushrooms
4. Discussion
4.1. Influence of SSP Supplementation on the Growth Rate of Mycelia
4.2. Influence of SSP Supplementation on the Morphological and Fruit Characteristics of P. eryngii
4.3. Influence of SSP Supplementation on the Nutrient Content of Mushrooms
4.4. Influence of SSP Supplementation on the Macronutrient Elements of Mushrooms
4.5. Influence of SSP Supplementation on the Micronutrient Elements of Mushrooms
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Substrate | Growth Rates over Time (mm d−1) | Growth Rate (mm d−1) | |||
---|---|---|---|---|---|
7~12 d | 12~17 d | 17~24 d | 24~31 d | ||
CK | 2.25 | 2.49 | 2.89 | 3.00 | 2.64 ± 0.14 d |
CK1 | 2.72 | 2.55 | 2.72 | 2.82 | 2.70 ± 0.18 cd |
T1 | 2.82 | 2.86 | 3.12 | 3.19 | 2.99 ± 0.14 a |
T2 | 2.65 | 2.26 | 2.99 | 3.38 | 2.81 ± 0.18 bc |
T3 | 2.53 | 2.35 | 2.82 | 2.77 | 2.62 ± 0.19 d |
T4 | 2.47 | 2.43 | 3.06 | 3.70 | 2.79 ± 0.20 ab |
T5 | 2.24 | 2.43 | 2.94 | 2.87 | 2.61 ± 0.10 d |
Substrate | Fresh Fruiting Body Yield (g bag−1) | Biological Efficiency (%) | Length of Fruit Body (cm) | Thickness of Stipe (cm) | Diameter of Pileus (cm) |
---|---|---|---|---|---|
CK | 349.8 ± 38.3 b | 74.0 ± 7.8 b | 14.9 ± 1.4 b | 5.4 ± 0.6 a | 4.5 ± 0.6 de |
CK1 | 390.1 ± 40.5 a | 82.8 ± 8.6 a | 16.4 ± 1.5 a | 5.5 ± 0.6 a | 4.7 ± 0.6 cd |
T1 | 399.1 ± 41.2 a | 84.5 ± 8.7 a | 16.0 ± 1.5 a | 5.7 ± 0.8 a | 5.4 ± 0.7 a |
T2 | 399.5 ± 44.2 a | 84.6 ± 9.4 a | 16.2 ± 1.6 a | 5.4 ± 0.9 a | 5.2 ± 0.7 ab |
T3 | 393.8 ± 35.2 a | 83.3 ± 7.4 a | 16.3 ± 2.0 a | 5.7 ± 0.5 a | 4.9 ± 0.8 bc |
T4 | 361.1 ± 34.1 b | 76.4 ± 7.2 b | 15.7 ± 1.6 a | 5.6 ± 0.6 a | 4.8 ± 0.5 cd |
T5 | 306.0 ± 45.0 c | 64.8 ± 9.5 c | 13.6 ± 1.5 c | 5.5 ± 0.6 a | 4.3 ± 0.5 e |
Substrate | Fiber | Fat | Ash | Protein | Polysaccharide |
---|---|---|---|---|---|
CK | 5.21 ± 0.02 g | 1.71 ± 0.01 c | 5.13 ± 0.02 c | 21.55 ± 0.15 d | 4.26 ± 0.01 d |
CK1 | 7.40 ± 0.01 b | 1.66 ± 0.01 d | 5.20 ± 0.01 b | 22.65 ± 0.25 c | 3.43 ± 0.02 f |
T1 | 6.34 ± 0.04 d | 2.11 ± 0.01 a | 4.88 ± 0.02 e | 21.15 ± 0.25 d | 4.54 ± 0.08 b |
T2 | 5.75 ± 0.04 f | 1.45 ± 0.01 f | 5.05 ± 0.02 d | 22.85 ± 0.25 bc | 4.96 ± 0.04 a |
T3 | 7.81 ± 0.04 a | 1.32 ± 0.01 g | 5.03 ± 0.07 d | 22.55 ± 0.05 c | 4.18 ± 0.01 e |
T4 | 6.54 ± 0.01 c | 1.90 ± 0.01 b | 5.32 ± 0.02 a | 23.85 ± 0.35 a | 4.43 ± 0.02 c |
T5 | 5.90 ± 0.03 e | 1.62 ± 0.01 e | 5.28 ± 0.02 a | 23.25 ± 0.25 b | 4.28 ± 0.03 d |
Treatments | CK | CK1 | T1 | T2 | T3 | T4 | T5 |
---|---|---|---|---|---|---|---|
Phosphorus (mg kg−1) | 736.0 ± 4.2 d | 741 ± 5.0 d | 766.0 ± 8.5 c | 738.0 ± 22.6 d | 744.5 ± 20.5 bc | 791.5 ± 0.7 ab | 797.5 ± 24.7 a |
Potassium (g kg−1) | 25.4 ± 0.02 bc | 25.9 ± 0.00 b | 25.7 ± 0.06 b | 24.5 ± 0.06 bc | 23.2 ± 0.00 d | 27.4 ± 0.18 a | 25.6 ± 0.04 b |
Magnesium (g kg−1) | 1.10 ± 0.03 c | 1.09 ± 0.03 c | 1.17 ± 0.00 b | 1.09 ± 0.01 c | 1.21 ± 0.01 b | 1.30 ± 0.08 a | 1.30 ± 0.01 a |
Sodium(mg kg−1) | 205.0 ± 1.4 d | 190 ± 0.0 e | 230.0 ± 0.0 c | 229.5 ± 14.8 c | 228.0 ± 4.2 c | 298.0 ± 21.2 a | 250.5 ± 7.8 b |
Calcium (mg kg−1) | 41.0 ± 0.3 e | 63.7 ± 0.6 b | 41.0 ± 0.4 e | 67.2 ± 0.8 a | 48.6 ± 0.4 d | 50.2 ± 0.1 d | 52.3 ± 2.8 c |
Treatments | CK | CK1 | T1 | T2 | T3 | T4 | T5 |
---|---|---|---|---|---|---|---|
Manganese (mg kg−1) | 6.77 ± 0.04 a | 6.35 ± 0.20 b | 5.58 ± 0.04 de | 5.99 ± 0.42 bc | 5.44 ± 0.11 e | 5.87 ± 0.13 cd | 6.27 ± 0.37 b |
Iron (mg kg−1) | 32.7 ± 0.5 e | 29.3 ± 0.6 f | 34.6 ± 0.0 cd | 35.1 ± 0.5 d | 36.6 ± 1.8 c | 44.1 ± 0.2 a | 39.2 ± 1.5 b |
Zinc (mg kg−1) | 80.2 ± 1.3 c | 72.7 ± 3.2 e | 82.0 ± 1.8 c | 76.4 ± 2.5 d | 87.8 ± 3.0 b | 80.5 ± 3.5 c | 91.3 ± 0.4 a |
Boron (mg kg−1) | 19.6 ± 0.5 b | 15.1 ± 0.0 d | 18.8 ± 0.8 bc | 14.2 ± 0.4 e | 18.6 ± 0.7 c | 21.6 ± 1.1 a | 21.4 ± 0.6 a |
Aluminum (mg kg−1) | 4.53 ± 0.25 d | 4.09 ± 0.00 e | 5.00 ± 0.23 b | 4.73 ± 0.16 c | 4.12 ± 0.08 e | 5.84 ± 0.05 a | 5.07 ± 0.12 b |
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Zhou, Y.; Li, Z.; Zhang, H.; Hu, Q.; Zou, Y. Potential Uses of Scallop Shell Powder as a Substrate for the Cultivation of King Oyster Mushroom (Pleurotus eryngii). Horticulturae 2022, 8, 333. https://doi.org/10.3390/horticulturae8040333
Zhou Y, Li Z, Zhang H, Hu Q, Zou Y. Potential Uses of Scallop Shell Powder as a Substrate for the Cultivation of King Oyster Mushroom (Pleurotus eryngii). Horticulturae. 2022; 8(4):333. https://doi.org/10.3390/horticulturae8040333
Chicago/Turabian StyleZhou, Yuanyuan, Zihao Li, Haijun Zhang, Qingxiu Hu, and Yajie Zou. 2022. "Potential Uses of Scallop Shell Powder as a Substrate for the Cultivation of King Oyster Mushroom (Pleurotus eryngii)" Horticulturae 8, no. 4: 333. https://doi.org/10.3390/horticulturae8040333