Next Article in Journal
Exploring the Role of Vertical and Horizontal Pathways in the Formation of Lettuce Plant Endospheric Bacterial Communities: A Comparative Study of Hydroponic and Soil Systems
Next Article in Special Issue
Extracellular Self-DNA Accumulation as a Potential Driver of Continuous Cropping Obstacle in Morchella sextelata and Morchella eximia
Previous Article in Journal
An Accurate Optimized Contour Segmentation Model for Green Spherical Fruits
Previous Article in Special Issue
Investigation of the Impact of Soil Physicochemical Properties and Microbial Communities on the Successful Cultivation of Morchella in Greenhouses
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Horticulturae
Volume 11
Issue 7
10.3390/horticulturae11070755
horticulturae-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Integration of Physiological and Comparative Transcriptomic Analyses Reveal the Toxicity Mechanism of p-Coumaric Acid on Morchella importuna

by
Qi Yin
1,
Wenchang Zhang
1,
Yingli Cai
2,
Xiaofei Shi
2,3,
Fuqiang Yu
3,
Jianzhuang Guo
4,
Xinhua He
5,6,
Peixin He
1,* and
Wei Liu
2,*
1
School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450002, China
2
Key Laboratory of Chemistry in Ethnic Medicinal Resources, School of Ethnic Medicine, Yunnan Minzu University, Kunming 650500, China
3
Germplasm Bank of Wild Species & Yunnan Key Laboratory for Fungal Diversity and Green Development, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
4
Agricultural Industry Development and Service Center of Luanchuan County, Luoyang 471500, China
5
School of Biological Sciences, University of Western Australia, Perth, WA 6009, Australia
6
Department of Land, Air and Water Resources, University of California at Davis, Davis, CA 95616, USA
*
Authors to whom correspondence should be addressed.
Horticulturae 2025, 11(7), 755; https://doi.org/10.3390/horticulturae11070755
Submission received: 16 April 2025 / Revised: 21 June 2025 / Accepted: 23 June 2025 / Published: 1 July 2025
(This article belongs to the Special Issue Morchella: Innovative Mushrooms from Forests to Farms, Then to Our Tables)
Browse Figures
Versions Notes

Abstract

p-coumaric acid (p-CA) is one of the main allelochemicals of cultivable Morchella mushrooms. However, its toxicity mechanism has not been elucidated. Therefore, we used physiological and comparative transcriptomic analyses to reveal its toxicity mechanism. The results suggest that the mycelial growth and sclerotial production of M. importuna were promoted under treatment with a low dosage of p-CA (10 μg/mL). The treatment induced moderate reactive oxygen species (ROS) accumulation, with an upregulation of genes associated with antioxidant regulation, energy supply and damage repair. In contrast, oxidative stress induced under treatment with a high dosage of p-CA (50 μg/mL) led to strain ageing. The contents of ROS were significantly increased, along with decreased peroxidase and catalase activity. Moreover, the genes associated with H2O2 synthesis were upregulated, while those responsible for H2O2 decomposition, non-enzymatic antioxidant components and damage repair were downregulated. Meanwhile, the carbohydrate and lipid metabolic pathways, and the signal transduction and cell division pathways, were impaired. Taken together, moderate stress induced under a low concentration of p-CA promotes the mycelial growth and sclerotial metamorphosis of M. importuna. This study provides new insights into the potential mechanisms of continuous cropping obstacles in the cultivation of morel mushrooms, which is of great importance for the practical aspects of mushroom cultivation.
Keywords: morel; phenolic acid; continuous cropping obstacle; oxidative stress; reactive oxygen species; strain ageing morel; phenolic acid; continuous cropping obstacle; oxidative stress; reactive oxygen species; strain ageing

Share and Cite

MDPI and ACS Style

Yin, Q.; Zhang, W.; Cai, Y.; Shi, X.; Yu, F.; Guo, J.; He, X.; He, P.; Liu, W. Integration of Physiological and Comparative Transcriptomic Analyses Reveal the Toxicity Mechanism of p-Coumaric Acid on Morchella importuna. Horticulturae 2025, 11, 755. https://doi.org/10.3390/horticulturae11070755

AMA Style

Yin Q, Zhang W, Cai Y, Shi X, Yu F, Guo J, He X, He P, Liu W. Integration of Physiological and Comparative Transcriptomic Analyses Reveal the Toxicity Mechanism of p-Coumaric Acid on Morchella importuna. Horticulturae. 2025; 11(7):755. https://doi.org/10.3390/horticulturae11070755

Chicago/Turabian Style

Yin, Qi, Wenchang Zhang, Yingli Cai, Xiaofei Shi, Fuqiang Yu, Jianzhuang Guo, Xinhua He, Peixin He, and Wei Liu. 2025. "Integration of Physiological and Comparative Transcriptomic Analyses Reveal the Toxicity Mechanism of p-Coumaric Acid on Morchella importuna" Horticulturae 11, no. 7: 755. https://doi.org/10.3390/horticulturae11070755

APA Style

Yin, Q., Zhang, W., Cai, Y., Shi, X., Yu, F., Guo, J., He, X., He, P., & Liu, W. (2025). Integration of Physiological and Comparative Transcriptomic Analyses Reveal the Toxicity Mechanism of p-Coumaric Acid on Morchella importuna. Horticulturae, 11(7), 755. https://doi.org/10.3390/horticulturae11070755

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop