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Horticulturae
Volume 11
Issue 12
10.3390/horticulturae11121540
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Proteome Insights into the Watercore Disorder on Pineapple Discovered Resistant Germplasm-Specific Accumulation of Cell Wall-Modifying and Defense Proteins

by
Dan Wang
1,†,
Yanli Yao
2,†,
Qian Yang
2,
Cunzhi Peng
1,
Lili Chang
1,
Qingsong Wu
2,
Xiaoqin Mo
1,
Bingqiang Xu
1,* and
Zheng Tong
1,3,*
1
Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
2
South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, China
3
Hainan Key Laboratory of Conservation and Utilization of Tropical Agricultural Biological Resources, Hainan Institute for Tropical Agricultural Resources, Haikou 571101, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Horticulturae 2025, 11(12), 1540; https://doi.org/10.3390/horticulturae11121540
Submission received: 6 November 2025 / Revised: 5 December 2025 / Accepted: 14 December 2025 / Published: 18 December 2025
(This article belongs to the Section Plant Pathology and Disease Management (PPDM))
Versions Notes

Abstract

Watercore disease, a physiological disorder in pineapple (Ananas comosus), manifests during late fruit development. Affected fruits develop water-soaked flesh and reduced storability. (1) Background: To explore underlying molecular mechanisms, comparative proteomic profiling was conducted in this study. (2) Methods: Data-independent acquisition (DIA) strategy was employed for comparative analysis between the resistant germplasm “35-1” and the susceptible germplasm “29-3”, as well as between the healthy and diseased “Paris”. (3) Results: Resistant (“35-1”) versus susceptible (“29-3”) germplasm analysis revealed differentially expressed proteins (DEPs) and unique proteins (SEPs) enriched in cell walls, secretory vesicles, and apoplast, functioning in cell wall loosening, hormone response, isoflavonoid biosynthesis, and farnesyl diphosphate biosynthesis. Healthy versus diseased “Paris” pulp analysis showed DEPs/SEPs enrichment in ribosomal small subunit biogenesis. These proteins form a central regulatory network potentially orchestrating tRNA synthesis, tubulin biosynthesis, and other carbohydrate metabolism. Partial protein overlap occurred in germplasm- and disease-derived differences. Resistant germplasm (“35-1”) and healthy “Paris” accumulated stress-responsive/resistant proteins and cell wall-modifying enzymes (e.g., phenylalanine ammonia-lyase, raffinose synthase, expansins, and mannan hydrolase). Susceptible germplasm (“29-3”) and diseased “Paris” exhibited prominent stress-responsive protein accumulation, such as alcohol dehydrogenase, 1-aminocyclopropane-1-carboxylate oxidase, and hypoxia-induced protein. (4) Conclusions: This comparative proteomics study identifies pineapple watercore resistance/susceptibility-associated proteins, providing a molecular basis for resistant germplasm development and disorder control.
Keywords: pineapple; watercore; comparative proteomics; stress responsive; cell wall reconstruction pineapple; watercore; comparative proteomics; stress responsive; cell wall reconstruction

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MDPI and ACS Style

Wang, D.; Yao, Y.; Yang, Q.; Peng, C.; Chang, L.; Wu, Q.; Mo, X.; Xu, B.; Tong, Z. Proteome Insights into the Watercore Disorder on Pineapple Discovered Resistant Germplasm-Specific Accumulation of Cell Wall-Modifying and Defense Proteins. Horticulturae 2025, 11, 1540. https://doi.org/10.3390/horticulturae11121540

AMA Style

Wang D, Yao Y, Yang Q, Peng C, Chang L, Wu Q, Mo X, Xu B, Tong Z. Proteome Insights into the Watercore Disorder on Pineapple Discovered Resistant Germplasm-Specific Accumulation of Cell Wall-Modifying and Defense Proteins. Horticulturae. 2025; 11(12):1540. https://doi.org/10.3390/horticulturae11121540

Chicago/Turabian Style

Wang, Dan, Yanli Yao, Qian Yang, Cunzhi Peng, Lili Chang, Qingsong Wu, Xiaoqin Mo, Bingqiang Xu, and Zheng Tong. 2025. "Proteome Insights into the Watercore Disorder on Pineapple Discovered Resistant Germplasm-Specific Accumulation of Cell Wall-Modifying and Defense Proteins" Horticulturae 11, no. 12: 1540. https://doi.org/10.3390/horticulturae11121540

APA Style

Wang, D., Yao, Y., Yang, Q., Peng, C., Chang, L., Wu, Q., Mo, X., Xu, B., & Tong, Z. (2025). Proteome Insights into the Watercore Disorder on Pineapple Discovered Resistant Germplasm-Specific Accumulation of Cell Wall-Modifying and Defense Proteins. Horticulturae, 11(12), 1540. https://doi.org/10.3390/horticulturae11121540

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