Linn. (Cannaceae) is used both as medicine and food. Traditionally, various parts of C. indica
are exploited to treat blood pressure, dropsy, fever, inflammatory diseases etc.
However, to date there is no reliable micropropagation protocol for C. indica
. We present here a regeneration technique C. indica
with banana micropropagation medium (BM). BM supplemented with 3% sucrose, 0.7% agar, and 0.17% NH4NO3 and different plant growth regulators like BAP (2 mg∙L−1) and NAA (0.5 mg∙L−1) was found to be effective in inducing callus. BM with BAP (2 mg∙L−1) was ideal for somatic embryogenesis and plantlet regeneration. After a period of 3 months, regenerated plantlets were successfully transferred to the field conditions. Appearance of somaclonal variation among the regenerated plants is a common problem which was assessed by DNA fingerprinting. To detect genetic fidelity in C. indica
, RAPD and ISSR markers were employed. Ten RAPD primers produced 60 amplicons, while 7 ISSR primers generated 45 bands in both in vitro
plantlets and mother plants. RAPD and ISSR analyses showed no evidence of polymorphism between parent plants and the regenerated plants as all the amplified products were found to be monomorphic.
This is an open access article distributed under the Creative Commons Attribution License
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited