Biological Detoxification of the Inhibitors in Corncob Acid Hydrolysate Using Aspergillus niger
Abstract
:1. Introduction
2. Materials and Methods
2.1. The Strain
2.2. Media
2.2.1. Potato Dextrose Agar Slant Culture Medium
2.2.2. Bran Seed Culture Medium
2.2.3. Liquid Culture Medium
2.2.4. Fermentation Medium
2.2.5. Lignocellulose Dilute Acid Hydrolysate Medium
2.3. Preparation of the A. niger M13 Spore Solution
2.4. Degradation Patterns of A. niger M13 Mycelium Balls in Response to a Single Inhibitor
2.5. Researching the Biodetoxification of Corncob Dilute Acid Hydrolysates by A. niger M13
2.6. Analytical Methods
3. Results and Discussion
3.1. Degradation Patterns of A. niger M13 on Acetic Acid
3.2. Degradation Patterns of A. niger M13 on Furfural
3.3. Degradation Patterns of A. niger M13 on HMF
3.4. Detoxification and Fermentation of Corncob Dilute Acid Hydrolysate by A. niger M13
3.4.1. A. niger M13 Mycelium Biodetoxification of Corncob Dilute Acid Hydrolysate
3.4.2. A. niger M13 Spores Biodetoxification of Corncob Dilute Acid Hydrolysate
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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24 h Removal Rate | 24 h Total Sugar Retention Rate | 48 h Removal Rate | 48 h Total Sugar Retention Rate | |||||
---|---|---|---|---|---|---|---|---|
Furfural | HMF | Acetic Acid | Furfural | HMF | Acetic Acid | |||
Corn cob | 100% | 100% | 31.5% | 100% | 100% | 100% | 100% | 97.5% |
Corn stalk | 100% | 100% | 38.0% | 98.9% | 100% | 100% | 100% | 86.8% |
Bagasse | 100% | 100% | 26.9% | 97.9% | 100% | 100% | 100% | 95.7% |
EFB | 100% | 100% | 34.3% | 85.0% | 100% | 100% | 100% | 48.0% |
Furfural (g/L) | HMF (g/L) | Acetic Acid (g/L) | Total Sugar (g/L) | |
---|---|---|---|---|
Corn cob | 1.35 | 0.37 | 7.50 | 79.06 |
Corn stalk | 0.95 | 0.40 | 4.16 | 55.23 |
Bagasse | 1.24 | 0.48 | 6.71 | 67.81 |
EFB | 1.51 | 0.47 | 7.73 | 41.89 |
Strain | Concentration (g/L) | Degradation Rate | Detoxification Rate (g/L/h) | References | ||||||
---|---|---|---|---|---|---|---|---|---|---|
Furfural | HMF | Acetic Acid | Furfural | HMF | Acetic Acid | Furfural | HMF | Acetic Acid | ||
Issatchenkia orientalis S-7 | 0.4 | -- | 4.0 | 100% | -- | 100% | 0.005 | -- | 0.05 | [37] |
Issatchenkia occidentalis CCTCC M 206097 | 0.016 | 0.02 | 3.3 | 100% | 100% | 6.1% | 0.00033 | 0.000417 | 0.00280 | [38] |
Aspergillus nidulans FLZ10 | 1.873 × 10−6 | 2.411 × 10−5 | 1.868 × 10−3 | 100% | 100% | 53.6% | 2.601 × 10−8 | 3.349 × 10−7 | 1.391 × 10−5 | [39] |
Amorphotheca resinae ZN1 | 0.8 | 2.27 | 4.0 | 100% | 100% | 35.0% | 0.1667 | 0.0236 | 0.01167 | [40] |
A. niger M13 | 1.35 | 0.37 | 7.5 | 100% | 100% | 100% | 0.1125 | 0.015 | 0.1566 | This study |
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Yin, J.; Wang, C.; Li, Y.; Lv, B.; Lv, H.; Xue, Y.; Wu, J.; Zhang, J. Biological Detoxification of the Inhibitors in Corncob Acid Hydrolysate Using Aspergillus niger. Fermentation 2023, 9, 854. https://doi.org/10.3390/fermentation9090854
Yin J, Wang C, Li Y, Lv B, Lv H, Xue Y, Wu J, Zhang J. Biological Detoxification of the Inhibitors in Corncob Acid Hydrolysate Using Aspergillus niger. Fermentation. 2023; 9(9):854. https://doi.org/10.3390/fermentation9090854
Chicago/Turabian StyleYin, Jinbao, Chen Wang, Yilian Li, Bo Lv, Haosheng Lv, Yuyuan Xue, Jing Wu, and Jianan Zhang. 2023. "Biological Detoxification of the Inhibitors in Corncob Acid Hydrolysate Using Aspergillus niger" Fermentation 9, no. 9: 854. https://doi.org/10.3390/fermentation9090854