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Open AccessArticle

Effect of Various Pretreatment Methods on Bioethanol Production from Cotton Stalks

1
Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, Zografou Campus, 15780 Athens, Greece
2
Chemical Process Engineering Laboratory, School of Chemical Engineering, National Technical University of Athens, Zografou Campus, 15780 Athens, Greece
3
Laboratory of Environmental Fuels and Hydrocarbons, Chemical Process and Energy Resources Institute (CPERI), Centre for Research and Technology Hellas (CERTH), 6th km Harilaou-Thermi Rd, 57001 Thessaloniki, Greece
*
Author to whom correspondence should be addressed.
These authors contributed equally to this paper.
Fermentation 2019, 5(1), 5; https://doi.org/10.3390/fermentation5010005
Received: 3 December 2018 / Revised: 12 December 2018 / Accepted: 20 December 2018 / Published: 1 January 2019
(This article belongs to the Special Issue Biomass Conversion: Fermentation Chemicals and Fuels)
Cotton stalks (CS) are considered a good candidate for fuel-ethanol production due to its abundance and high carbohydrate content, but the direct conversion without pretreatment always results in extremely low yields due to the recalcitrant nature of lignocelluloses. The present study was undertaken to investigate the effect of various chemical and physicochemical pretreatment methods, i.e., alkali, microwave-assisted acid, organosolv, hydrothermal treatment, and sequentially organosolv and hydrothermal pretreatment, on chemical composition of CS and subsequent ethanol production applying pre-hydrolysis and simultaneous saccharification and fermentation (PSSF) at high solid loading. The best results in terms of ethanol production were achieved by the sequential combination of organosolv and hydrothermal pretreatment (32.3 g/L, using 15% w/v substrate concentration and 6 h pre-hydrolysis) with an improvement of 32% to 50% in ethanol production compared to the other pretreatments. Extending pre-hydrolysis time to 14 h and increasing substrate concentration to 20% w/v, ethanol production reached 47.0 g/L (corresponding to an ethanol yield of 52%) after 30 h of fermentation. View Full-Text
Keywords: cotton stalks; alkali pretreatment; hydrothermal treatment; microwave-assisted acid pretreatment; organosolv; bioethanol; high solids pre-hydrolysis and simultaneous saccharification and fermentation cotton stalks; alkali pretreatment; hydrothermal treatment; microwave-assisted acid pretreatment; organosolv; bioethanol; high solids pre-hydrolysis and simultaneous saccharification and fermentation
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Dimos, K.; Paschos, T.; Louloudi, A.; Kalogiannis, K.G.; Lappas, A.A.; Papayannakos, N.; Kekos, D.; Mamma, D. Effect of Various Pretreatment Methods on Bioethanol Production from Cotton Stalks. Fermentation 2019, 5, 5.

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