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A Tool to Design Bridging Oligos Used to Detect Pseudouridylation Sites on RNA after CMC Treatment

UMR7216 Épigénétique et Destin Cellulaire, CNRS, Université Paris Cité, F-75013 Paris, France
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Y-h. Taguchi
Non-Coding RNA 2022, 8(5), 63; https://doi.org/10.3390/ncrna8050063
Received: 29 August 2022 / Revised: 16 September 2022 / Accepted: 21 September 2022 / Published: 23 September 2022
(This article belongs to the Collection Non-coding RNA Methods)
Pseudouridylation is one of the most abundant modifications found in RNAs. To identify the Pseudouridylation sites (Psi) in RNAs, several techniques have been developed, but the most common and robust is the CMC (N-cyclohexyl-N′-(2-morpholinoethyl)carbodiimide) treatment, which consists in the addition of an adduct on Psi that inhibits the reverse transcription. Here, we describe a turnkey method and a tool to design the bridging oligo (DBO), which is somewhat difficult to design. Finally, we propose a trouble-shooting guide to help users. View Full-Text
Keywords: CMC treatment; Oligonucleotide design; tool; Pseudouridylation; snoRNA CMC treatment; Oligonucleotide design; tool; Pseudouridylation; snoRNA
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MDPI and ACS Style

Bogard, B.; Tellier, G.; Francastel, C.; Hubé, F. A Tool to Design Bridging Oligos Used to Detect Pseudouridylation Sites on RNA after CMC Treatment. Non-Coding RNA 2022, 8, 63. https://doi.org/10.3390/ncrna8050063

AMA Style

Bogard B, Tellier G, Francastel C, Hubé F. A Tool to Design Bridging Oligos Used to Detect Pseudouridylation Sites on RNA after CMC Treatment. Non-Coding RNA. 2022; 8(5):63. https://doi.org/10.3390/ncrna8050063

Chicago/Turabian Style

Bogard, Baptiste, Gilles Tellier, Claire Francastel, and Florent Hubé. 2022. "A Tool to Design Bridging Oligos Used to Detect Pseudouridylation Sites on RNA after CMC Treatment" Non-Coding RNA 8, no. 5: 63. https://doi.org/10.3390/ncrna8050063

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