Synthesis of Cationic Quaternized Nanolevan Derivative for Small Molecule and Nucleic Acid Delivery
Abstract
:1. Introduction
2. Results and Discussion
2.1. Chemical Modification of Levan and Characterization
2.2. Formation of QA−Levan/DNA Polyplexes
2.3. Drug Delivery Application of QA−Levan
2.4. Cytotoxicity to Human Cancer Cell Line
3. Conclusions
4. Materials and Methods
4.1. Chemicals
4.2. Preparation of Levan
4.3. Preparation of Quaternary Ammonium Levan (QA-Levan)
4.4. Characterization
4.4.1. Fourier Transform Infrared (FT-IR) Analysis
4.4.2. CHN Analysis
4.4.3. 1H-NMR
4.4.4. Zeta Sizer
4.5. Formation of QA-Levan/DNA Polyplexes
4.6. Encapsulation of Small Molecules
4.7. Cytotoxicity
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sample | %N | %C | %H | N/C |
---|---|---|---|---|
Levan | ND | 42.29 | 6.92 | ND |
4%GTMAC modified levan | 0.70 | 39.59 | 6.46 | 0.02 |
8%GTMAC modified levan | 1.24 | 40.32 | 6.65 | 0.03 |
20%GTMAC modified levan | 2.86 | 41.03 | 7.34 | 0.07 |
40%GTMAC modified levan | 2.45 | 36.72 | 6.92 | 0.07 |
Sample | Diameter (nm) | ξ-Potential Mean ± SEM (mV) |
---|---|---|
Levan | 157.9 ± 0.2 | −7.05 ± 0.19 |
QA-levan | 193.8 ± 1.5 | 53.7 ± 0.53 |
QA-levan/DNA complex | 170.7 ± 1.7 | 47.9 ± 0.70 |
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Charoenwongphaibun, C.; Lorthongpanich, C.; Septham, P.; Wangpaiboon, K.; Panpetch, P.; Pichyangkura, R.; Charoenwongpaiboon, T.; Kuttiyawong, K. Synthesis of Cationic Quaternized Nanolevan Derivative for Small Molecule and Nucleic Acid Delivery. Gels 2023, 9, 188. https://doi.org/10.3390/gels9030188
Charoenwongphaibun C, Lorthongpanich C, Septham P, Wangpaiboon K, Panpetch P, Pichyangkura R, Charoenwongpaiboon T, Kuttiyawong K. Synthesis of Cationic Quaternized Nanolevan Derivative for Small Molecule and Nucleic Acid Delivery. Gels. 2023; 9(3):188. https://doi.org/10.3390/gels9030188
Chicago/Turabian StyleCharoenwongphaibun, Chonnipha, Chanchao Lorthongpanich, Prapasri Septham, Karan Wangpaiboon, Pawinee Panpetch, Rath Pichyangkura, Thanapon Charoenwongpaiboon, and Kamontip Kuttiyawong. 2023. "Synthesis of Cationic Quaternized Nanolevan Derivative for Small Molecule and Nucleic Acid Delivery" Gels 9, no. 3: 188. https://doi.org/10.3390/gels9030188