Ubiquitin Degradation of the AICAR Transformylase/IMP Cyclohydrolase Ade16 Regulates the Sexual Reproduction of Cryptococcus neoformans
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains and Growth Conditions
2.2. Quantitative Real-Time PCR
2.3. Generation of Fluorescence Strains
2.4. Yeast Two-Hybrid Assays
2.5. Generation of Tagged Protein Strains
2.6. Generation of ADE16 Interference and Overexpression Strains
2.7. Analysis of Melanin and Capsule
2.8. Mating Assay
2.9. Statistical Analysis
3. Results
3.1. Interaction Profile and Expression Pattern of ADE16 during Mating of C. neoformans
3.2. Ade16 Interacts with Fbp1
3.3. The Ade16 Stability Depends on Fbp1 Function
3.4. ADE16 Is Required for Normal Growth of C. neoformans
3.5. ADE16 Overexpression Increases Capsule Size in C. neoformans
3.6. Ade16 Regulates Sexual Reproduction of C. neoformans
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Accession | Description | Average fbp1Δ/H99 | No. of PEST Domain |
---|---|---|---|
CNAG_05514 | Uncharacterized protein | 1.899038 | 0 |
CNAG_01974 | Ribosomal protein | 1.631707 | 2 |
CNAG_06195 | Uncharacterized protein | 1.595274 | 0 |
CNAG_00700 | ATIC Ade16 | 1.544354 | 0 |
CNAG_05498 | Uncharacterized protein | 1.541769 | 1 |
CNAG_02860 | Endo-1,3(4)-beta-glucanase | 1.522101 | 2 |
CNAG_01019 | Superoxide dismutase | 1.470508 | 0 |
CNAG_02344 | Uracil phosphoribosyltransferase | 1.431079 | 1 |
CNAG_02738 | Uncharacterized protein | 1.400934 | 0 |
CNAG_01109 | Uncharacterized protein | 1.384226 | 1 |
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Han, L.; Wu, Y.; Xiong, S.; Liu, T. Ubiquitin Degradation of the AICAR Transformylase/IMP Cyclohydrolase Ade16 Regulates the Sexual Reproduction of Cryptococcus neoformans. J. Fungi 2023, 9, 699. https://doi.org/10.3390/jof9070699
Han L, Wu Y, Xiong S, Liu T. Ubiquitin Degradation of the AICAR Transformylase/IMP Cyclohydrolase Ade16 Regulates the Sexual Reproduction of Cryptococcus neoformans. Journal of Fungi. 2023; 9(7):699. https://doi.org/10.3390/jof9070699
Chicago/Turabian StyleHan, Liantao, Yujuan Wu, Sichu Xiong, and Tongbao Liu. 2023. "Ubiquitin Degradation of the AICAR Transformylase/IMP Cyclohydrolase Ade16 Regulates the Sexual Reproduction of Cryptococcus neoformans" Journal of Fungi 9, no. 7: 699. https://doi.org/10.3390/jof9070699