RNA-Seq Provides Insights into the Mechanisms Underlying Ilyonectria robusta Responding to Secondary Metabolites of Bacillus methylotrophicus NJ13
Abstract
:1. Introduction
2. Materials and Methods
2.1. Isolation and Culture Conditions of Strains
2.2. Preparation of CBLJ-3’s Conidia and Fermentation Filtrates without NJ13’s Cells
2.3. Antifungal Activity Assay and Microscopic Observation
2.4. Effect of NJ13’s SMs on the Germination of I. robusta Conidia
2.5. Measurement of Mycelial Extracellular Conductivity
2.6. Detection of Malondialdehyde (MDA) in CBLJ-3’s Mycelia in the Presence of NJ13’s SMs
2.7. Measurement of the Glucose Produced by CBLJ-3 in the Presence of NJ13’s SMs
2.8. Control Effect on Ginseng Rusty Roots Infected by I. robusta Using NJ13’s SMs
2.9. RNA Extraction and Sequencing
2.10. Data Analysis
2.11. QRT-PCR Validation
2.12. Statistical Analysis
3. Results
3.1. SMs from B. methylotrophicus NJ13 Inhibited CBLJ-3
3.2. Effect of NJ13’s SMs on the Germination of CBLJ-3’s Conidia
3.3. Effect of NJ13’s SMs on the Cell Membrane of CBLJ-3
3.4. Glucose Absorption of Strain CBLJ-3 Was Affected by NJ13 Sterile Filtrates
3.5. In Vivo Test of Antifungal Activity in Panax ginseng Roots
3.6. Quality Control and Quantification of Gene Expression Levels
3.7. Overall Analysis of Differentially Expressed Genes (DEGs)
3.8. GO Enrichment Analysis of DEGs
3.9. KEGG Enrichment Analysis of DEGs
3.10. Analysis of DEGs in Response to Stress Caused by B. methylotrophicus NJ13’s SMs
3.11. Validation of Transcriptomic Results Using QRT-PCR
4. Discussion
4.1. Effect of the Antifungal Activity of B. methylotrophicus NJ13’s SMs on CBLJ-3
4.2. Effects of B. methylotrophicus NJ13’s SMs on the Cellular Structure of CBLJ-3
4.3. Analysis of the Effect of B. methylotrophicus NJ13’s SMs on CBLJ-3’s Metabolism
4.4. Unanswered Questions and Future Possibilities
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Li, X.; Li, M.; Liu, X.; Jiang, Y.; Zhao, D.; Gao, J.; Wang, Z.; Jiang, Y.; Chen, C. RNA-Seq Provides Insights into the Mechanisms Underlying Ilyonectria robusta Responding to Secondary Metabolites of Bacillus methylotrophicus NJ13. J. Fungi 2022, 8, 779. https://doi.org/10.3390/jof8080779
Li X, Li M, Liu X, Jiang Y, Zhao D, Gao J, Wang Z, Jiang Y, Chen C. RNA-Seq Provides Insights into the Mechanisms Underlying Ilyonectria robusta Responding to Secondary Metabolites of Bacillus methylotrophicus NJ13. Journal of Fungi. 2022; 8(8):779. https://doi.org/10.3390/jof8080779
Chicago/Turabian StyleLi, Xiang, Mengtao Li, Xiangkai Liu, Yilin Jiang, Dongfang Zhao, Jie Gao, Zhenhui Wang, Yun Jiang, and Changqing Chen. 2022. "RNA-Seq Provides Insights into the Mechanisms Underlying Ilyonectria robusta Responding to Secondary Metabolites of Bacillus methylotrophicus NJ13" Journal of Fungi 8, no. 8: 779. https://doi.org/10.3390/jof8080779