Rapid Diagnosis of Central Nervous System Scedosporiosis by Specific Quantitative Polymerase Chain Reaction Applied to Formalin-Fixed, Paraffin-Embedded Tissue
Abstract
:1. Introduction
2. Case Presentation
3. Materials and Methods
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Literature | Biopsy | Histopathology | Culture | Microscopy of Cultured Material | PCR | Sequencing | Comments |
---|---|---|---|---|---|---|---|
Buzina et al., 2006 [8] | + | o | Blood: − BAL:− Biopsy: + | + | Culture: + | Culture: + | |
Mursch et al., 2006 [24] | + | o | CSF: − Biopsy: + | o | o | Culture: + | |
Caggiano et al., 2011 [9] | + | (+) | Biopsy:+ | + | Molecular analysis of culture was performed but not further specified. | ||
Nakamura et al., 2011 [21] | o | o | BAL: + | BAL: + | BAL: + | BAL: + | |
Tammer et al., 2011 [25] | + | (+) | Biopsy: + | + | − | Culture: + | |
Henao-Martinez et al., 2013 [26] Case 1 | o | o | FESS: + | + | o | o | |
Henao-Martinez et al., 2013 [26] Case 2 | −/+ | (+) | CSF: + | o | o | o | 1st Biopsy − |
Lin et al., 2013 [27,28] | + | (+) | Biopsy: + | o | o | o | |
Wilson et al., 2013 | + | (+) | Biopsy: + Sputum: + | + | Biopsy: + | + | |
Williams et al., 2016 [29] | + | − | CSF: − Biopsy: + | o | CSF: − Biopsy: + | o | |
Signore et al., 2017 [13] | −/+ | −/+ | Blood: − Biopsy: + | o | Biopsy: − Blood: − | Tissue: + | 1st Biopsy − |
Lee et al., 2018 [14] | + | o | Sputum:− CSF:− Biopsy:+ | o | o | o | |
Sudke et al., 2020 [30] | + | + | Culture + | + | o | o |
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Lauerer, R.J.; Rosenow, E.; Beschorner, R.; Hempel, J.-M.; Naros, G.; Hofmann, A.; Berger, K.; Sartor-Pfeiffer, J.; Mengel, A.; Ziemann, U.; et al. Rapid Diagnosis of Central Nervous System Scedosporiosis by Specific Quantitative Polymerase Chain Reaction Applied to Formalin-Fixed, Paraffin-Embedded Tissue. J. Fungi 2022, 8, 19. https://doi.org/10.3390/jof8010019
Lauerer RJ, Rosenow E, Beschorner R, Hempel J-M, Naros G, Hofmann A, Berger K, Sartor-Pfeiffer J, Mengel A, Ziemann U, et al. Rapid Diagnosis of Central Nervous System Scedosporiosis by Specific Quantitative Polymerase Chain Reaction Applied to Formalin-Fixed, Paraffin-Embedded Tissue. Journal of Fungi. 2022; 8(1):19. https://doi.org/10.3390/jof8010019
Chicago/Turabian StyleLauerer, Robert J., Emely Rosenow, Rudi Beschorner, Johann-Martin Hempel, Georgios Naros, Anna Hofmann, Katharina Berger, Jennifer Sartor-Pfeiffer, Annerose Mengel, Ulf Ziemann, and et al. 2022. "Rapid Diagnosis of Central Nervous System Scedosporiosis by Specific Quantitative Polymerase Chain Reaction Applied to Formalin-Fixed, Paraffin-Embedded Tissue" Journal of Fungi 8, no. 1: 19. https://doi.org/10.3390/jof8010019