Destruxin A Interacts with Aminoacyl tRNA Synthases in Bombyx mori
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Lines and Compounds
2.2. Preparation of Protein and Biolayer Interferometry (BLI)
2.3. Homology Modeling and Molecular Docking
2.4. Assessment of Gene Expression
2.5. Detection of Soluble Protein and Free Amino-Acid Content
3. Results
3.1. Interaction of DA with Six Silkworm ARSs
3.2. Analysis of Soluble Protein, Free Amino Acids, and Gene Expression Levels of ARSs in Bm12 Cells
3.3. Interaction Mode of DA with Silkworm ARSs
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene | Primer (5′→3′) |
---|---|
BmAlaRS | F: ACATGGCGTATCGTGTCTTGGC |
R: TCTGACGCATATCGCACAGCTC | |
BmCysRS | F: ACGCCACACCGCAAGACAAC |
R: CCATCCACCGCCGCAACC | |
BmMetRS | F: TCTGGCTGACAGGTTCGTGGAG |
R: CCGCACTTGTCGCACTGGTC | |
BmValRS | F: ACCTGTGGCTCTACGACCTGTG |
R: CGGTTACGAACGGCATGAAGGG | |
BmIleRS | F: AGGGGCGCGTTTGAAAGGTG |
R: CTGGTCACTGCCTGTAGCTTGG | |
BmGluProRS | F: AGCAACCGCAACCTTTCCTGAG |
R: CGCCGCCAGCAAACTTCTCC | |
GAPDH | F: ATGTTTGTTGTGGGTGTTA |
R: GTAGAGGCAGGAATGATT |
Proteins | Kon (1/M·s) 1 | Kdis (1/s) 2 | KD (M) 3 |
---|---|---|---|
BmAlaRS | 1.15 × 102 | 3.66 × 10−2 | 3.18 × 10−4 |
BmCysRS | 2.10 × 102 | 1.49 × 10−2 | 7.12 × 10−5 |
BmMetRS | 3.07 × 102 | 2.03 × 10−2 | 6.61 × 10−5 |
BmValRS | 5.72 × 102 | 2.32 × 10−2 | 4.39 × 10−5 |
BmIleRS | 1.15 × 102 | 6.80 × 10−2 | 5.90 × 10−4 |
BmGluProRS | 9.39 × 10 | 5.67 × 10−2 | 6.04 × 10−4 |
Proteins | Docking Free Energy (kcal/mol) |
---|---|
BmAlaRS | −8.87 |
BmGluProRS | −7.78 |
BmCysRS | −8.97 |
BmIleRS | −7.49 |
BmMetRS | −8.25 |
BmValRS | −9.23 |
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Wang, J.; Berestetskiy, A.; Hu, Q. Destruxin A Interacts with Aminoacyl tRNA Synthases in Bombyx mori. J. Fungi 2021, 7, 593. https://doi.org/10.3390/jof7080593
Wang J, Berestetskiy A, Hu Q. Destruxin A Interacts with Aminoacyl tRNA Synthases in Bombyx mori. Journal of Fungi. 2021; 7(8):593. https://doi.org/10.3390/jof7080593
Chicago/Turabian StyleWang, Jingjing, Alexander Berestetskiy, and Qiongbo Hu. 2021. "Destruxin A Interacts with Aminoacyl tRNA Synthases in Bombyx mori" Journal of Fungi 7, no. 8: 593. https://doi.org/10.3390/jof7080593