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Open AccessArticle

Laser Capture Microdissection-Assisted Protein Biomarker Discovery from Coccidioides-Infected Lung Tissue

1
School of Life Sciences, Mayo Clinic Collaborative Research Building, Arizona State University, Scottsdale, AZ 85259, USA
2
Department of Health Sciences Research, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
3
Department of Laboratory Medicine and Pathology, Mayo Clinic, Phoenix, AZ 85054, USA
*
Author to whom correspondence should be addressed.
J. Fungi 2020, 6(4), 365; https://doi.org/10.3390/jof6040365
Received: 3 November 2020 / Revised: 10 December 2020 / Accepted: 12 December 2020 / Published: 14 December 2020
(This article belongs to the Special Issue Coccidioides and Coccidioidomycosis 2020)
Laser capture microdissection (LCM) coupled to label-free quantitative mass spectrometry is a viable strategy to identify biomarkers from infected tissues. In this study, LCM was employed to take a “snapshot” of proteins produced in vivo during Coccidiodies spp. infection in human lungs. Proteomic analysis of LCM lung sections revealed hundreds of hosts and Coccidioidal proteins. Twenty-seven highly abundant Coccidioides spp. proteins were identified which do not share significant sequence orthology with human proteins. Three of the 27 Coccidioidal proteins are also potential Coccidoides-specific biomarkers, as they also do not share sequence homology to any other pathogenic fungus or microbe. Gene ontology analysis of the 27 biomarker candidate proteins revealed enriched hydrolase activity and increased purine and carbohydrate metabolism functions. Finally, we provide proteomic evidence that all 27 biomarker candidates are produced by the fungus when grown in vitro in a media- and growth-phase dependent manner. View Full-Text
Keywords: Coccidioides; Valley Fever; biomarker; mass spectrometry; laser capture microdissection Coccidioides; Valley Fever; biomarker; mass spectrometry; laser capture microdissection
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MDPI and ACS Style

Mitchell, N.M.; Dasari, S.; Grys, T.E.; Lake, D.F. Laser Capture Microdissection-Assisted Protein Biomarker Discovery from Coccidioides-Infected Lung Tissue. J. Fungi 2020, 6, 365. https://doi.org/10.3390/jof6040365

AMA Style

Mitchell NM, Dasari S, Grys TE, Lake DF. Laser Capture Microdissection-Assisted Protein Biomarker Discovery from Coccidioides-Infected Lung Tissue. Journal of Fungi. 2020; 6(4):365. https://doi.org/10.3390/jof6040365

Chicago/Turabian Style

Mitchell, Natalie M.; Dasari, Surendra; Grys, Thomas E.; Lake, Douglas F. 2020. "Laser Capture Microdissection-Assisted Protein Biomarker Discovery from Coccidioides-Infected Lung Tissue" J. Fungi 6, no. 4: 365. https://doi.org/10.3390/jof6040365

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