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Open AccessArticle

Paracoccidioides HSP90 Can Be Found in the Cell Surface and Is a Target for Antibodies with Therapeutic Potential

1
Institute of Tropical Medicine, Department of Dermatology, Faculty of Medicine, University of São Paulo, São Paulo 05403-000, SP, Brazil
2
Institute of Biological Sciences, University of Brasília, Brasília 70910-900, DF, Brazil
3
Karan Technologies Research and Development, Brasília 70632-200, DF, Brazil
4
Faculty of Medicine, University of Brasília, Brasília 70910-900, DF, Brazil
5
Bacteriology Laboratory, Butantan Institute, São Paulo 05503-900, SP, Brazil
6
Department of Molecular Microbiology & Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA
7
Faculty of Ceilândia, University of Brasília, Brasília 72220-275, DF, Brazil
8
Graduate Program in Genomic Sciences and Biotechnology, Catholic University of Brasília, Brasília 70790-160, DF, Brazil
*
Author to whom correspondence should be addressed.
J. Fungi 2020, 6(4), 193; https://doi.org/10.3390/jof6040193
Received: 9 August 2020 / Revised: 5 September 2020 / Accepted: 10 September 2020 / Published: 28 September 2020
(This article belongs to the Special Issue Monoclonal Antibodies as Tools to Diagnose and Treat Fungal Infection)
Paracoccidioidomycosis (PCM) is one of the most frequent systemic mycoses in Latin America. It affects mainly male rural workers in impoverished regions, and the therapy can last up to two years or use drugs that are very toxic. Given the need for novel safe and effective approaches to treat PCM, we have been developing monoclonal antibodies (mAbs) that could be used not only to block specific fungal targets, but also modulate the host’s antifungal immunity. In this work we show the generation of and promising results with an mAb against Heat Shock Protein (HSP)90, a molecular chaperone that is an important virulence factor in fungi. Using recombinant Paracoccidioides lutzii (Pb01) and P. brasiliensis (Pb18) HSP90 proteins produced in E. coli, we immunized mice and generated polyclonal antibodies and an IgG1 hybridoma mAb. The proteins were very immunogenic and both the polyclonal serum and mAb were used in immunofluorescence experiments, which showed binding of antibodies to the yeast cell surface. The mAb successfully opsonized P. lutzii and P. brasiliensis cells in co-incubations with J774.16 macrophage-like cells. Our results suggest that this mAb could serve as the basis for new immunotherapy regimens for PCM. View Full-Text
Keywords: monoclonal antibody; Paracoccidioides spp.; HSP90 monoclonal antibody; Paracoccidioides spp.; HSP90
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MDPI and ACS Style

Moura, Á.N.D.; Oliveira, D.S.L.d.; Paredes, V.; Rocha, L.B.; Oliveira, F.F.M.d.; Lessa, G.M.; Riasco-Palacios, J.F.; Casadevall, A.; Albuquerque, P.; Felipe, M.S.S.; Piazza, R.M.F.; Nicola, A.M. Paracoccidioides HSP90 Can Be Found in the Cell Surface and Is a Target for Antibodies with Therapeutic Potential. J. Fungi 2020, 6, 193.

AMA Style

Moura ÁND, Oliveira DSLd, Paredes V, Rocha LB, Oliveira FFMd, Lessa GM, Riasco-Palacios JF, Casadevall A, Albuquerque P, Felipe MSS, Piazza RMF, Nicola AM. Paracoccidioides HSP90 Can Be Found in the Cell Surface and Is a Target for Antibodies with Therapeutic Potential. Journal of Fungi. 2020; 6(4):193.

Chicago/Turabian Style

Moura, Ágata N.D.; Oliveira, Diane S.L.d.; Paredes, Verenice; Rocha, Letícia B.; Oliveira, Fabiana F.M.d.; Lessa, Gustavo M.; Riasco-Palacios, Juan F.; Casadevall, Arturo; Albuquerque, Patrícia; Felipe, Maria S.S.; Piazza, Roxane M.F.; Nicola, André M. 2020. "Paracoccidioides HSP90 Can Be Found in the Cell Surface and Is a Target for Antibodies with Therapeutic Potential" J. Fungi 6, no. 4: 193.

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