The High Osmolarity Glycerol (HOG) Pathway Functions in Osmosensing, Trap Morphogenesis and Conidiation of the Nematode-Trapping Fungus Arthrobotrys oligospora

Kuo, Chih-Yen; Chen, Sheng-An; Hsueh, Yen-Ping

doi:10.3390/jof6040191

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The High Osmolarity Glycerol (HOG) Pathway Functions in Osmosensing, Trap Morphogenesis and Conidiation of the Nematode-Trapping Fungus Arthrobotrys oligospora

Chih-Yen Kuo

^1,2,†,

Sheng-An Chen

^2,† and

Yen-Ping Hsueh

^1,2,*

Molecular and Cell Biology, Taiwan International Graduate Program, Academia Sinica and Graduate Institute of Life Sciences, National Defense Medical Center, Taipei 11490, Taiwan

Institute of Molecular Biology, Academia Sinica, Nangang, Taipei 11490, Taiwan

Author to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

J. Fungi 2020, 6(4), 191; https://doi.org/10.3390/jof6040191

Received: 7 September 2020 / Revised: 21 September 2020 / Accepted: 25 September 2020 / Published: 27 September 2020

(This article belongs to the Special Issue Cell Surface Receptors on Fungal Pathogens)

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Abstract

Hog1, a mitogen-activated protein kinase (MAPK), has been identified in diverse fungal species, and it regulates various cellular processes, such as osmoadaptation, nutrient-sensing, and pathogenesis. However, the roles that Hog1 plays in nematode-trapping fungi were previously unclear. Here, we characterized orthologs of Saccharomyces cerevisiae Hog1 and membrane mucin Msb2 in the nematode-trapping fungus Arthrobotrys oligospora. We generated gene deletion mutants of HOG1 and MSB2 in A. oligospora, and characterized their roles in osmosensing, growth, and trap morphogenesis. We found that both hog1 and msb2 mutants were highly sensitive to high osmolarity. Predation analyses further revealed that hog1 and msb2 deletion caused a reduction in trap formation and predation efficiency. Furthermore, HOG1 is required for conidiation in A. oligospora, demonstrating its critical role in this developmental pathway. In summary, this study demonstrated that the conserved Hog1 and Msb2 govern physiology, growth and development in the nematode-trapping fungus A. oligospora. View Full-Text

Keywords: Arthrobotrys oligospora; high osmolarity glycerol (HOG) pathway; Hog1 mitogen-activated protein kinase; osmolarity; nematocidal activity; signaling mucin Msb2

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Kuo, C.-Y.; Chen, S.-A.; Hsueh, Y.-P. The High Osmolarity Glycerol (HOG) Pathway Functions in Osmosensing, Trap Morphogenesis and Conidiation of the Nematode-Trapping Fungus Arthrobotrys oligospora. J. Fungi 2020, 6, 191. https://doi.org/10.3390/jof6040191

AMA Style

Kuo C-Y, Chen S-A, Hsueh Y-P. The High Osmolarity Glycerol (HOG) Pathway Functions in Osmosensing, Trap Morphogenesis and Conidiation of the Nematode-Trapping Fungus Arthrobotrys oligospora. Journal of Fungi. 2020; 6(4):191. https://doi.org/10.3390/jof6040191

Chicago/Turabian Style

Kuo, Chih-Yen, Sheng-An Chen, and Yen-Ping Hsueh. 2020. "The High Osmolarity Glycerol (HOG) Pathway Functions in Osmosensing, Trap Morphogenesis and Conidiation of the Nematode-Trapping Fungus Arthrobotrys oligospora" Journal of Fungi 6, no. 4: 191. https://doi.org/10.3390/jof6040191

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The High Osmolarity Glycerol (HOG) Pathway Functions in Osmosensing, Trap Morphogenesis and Conidiation of the Nematode-Trapping Fungus Arthrobotrys oligospora

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