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Open AccessArticle

Exploring the Antibacterial Activity of Pestalotiopsis spp. under Different Culture Conditions and Their Chemical Diversity Using LC–ESI–Q–TOF–MS

1
Laboratory of Tropical Bioorganic Chemistry, Faculty of Natural, Exact Sciences and Technology, University of Panama, Panama 0824, Panama
2
Vicerrectoría de Investigación y Postgrado, University of Panama, Panama 0824, Panama
3
Department of Microbiology, Faculty of Natural, Exact Sciences and Technology, University of Panama, Panama 0824, Panama
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
J. Fungi 2020, 6(3), 140; https://doi.org/10.3390/jof6030140
Received: 16 July 2020 / Revised: 14 August 2020 / Accepted: 17 August 2020 / Published: 19 August 2020
As a result of the capability of fungi to respond to culture conditions, we aimed to explore and compare the antibacterial activity and chemical diversity of two endophytic fungi isolated from Hyptis dilatata and cultured under different conditions by the addition of chemical elicitors, changes in the pH, and different incubation temperatures. Seventeen extracts were obtained from both Pestalotiopsis mangiferae (man-1 to man-17) and Pestalotiopsis microspora (mic-1 to mic-17) and were tested against a panel of pathogenic bacteria. Seven extracts from P. mangiferae and four extracts from P. microspora showed antibacterial activity; while some of these extracts displayed a high-level of selectivity and a broad-spectrum of activity, Pseudomonas aeruginosa was the most inhibited microorganism and was selected to determine the minimal inhibitory concentration (MIC). The MIC was determined for extracts man-6 (0.11 μg/mL) and mic-9 (0.56 μg/mL). Three active extracts obtained from P. mangiferae were analyzed by Liquid Chromatography-Electrospray Ionization-Quadrupole-Time of Flight-Mass Spectrometry (LC–ESI–Q–TOF–MS) to explore the chemical diversity and the variations in the composition. This allows us to propose structures for some of the determined molecular formulas, including the previously reported mangiferaelactone (1), an antibacterial compound. View Full-Text
Keywords: Endophytic fungi; Hyptis dilatata; Pestalotiopsis mangiferae; Pestalotiopsis microspora; chemical elicitors; antibacterial activity; LC–ESI–Q–TOF–MS Endophytic fungi; Hyptis dilatata; Pestalotiopsis mangiferae; Pestalotiopsis microspora; chemical elicitors; antibacterial activity; LC–ESI–Q–TOF–MS
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MDPI and ACS Style

Aguilar-Pérez, M.M.; Torres-Mendoza, D.; Vásquez, R.; Rios, N.; Cubilla-Rios, L. Exploring the Antibacterial Activity of Pestalotiopsis spp. under Different Culture Conditions and Their Chemical Diversity Using LC–ESI–Q–TOF–MS. J. Fungi 2020, 6, 140. https://doi.org/10.3390/jof6030140

AMA Style

Aguilar-Pérez MM, Torres-Mendoza D, Vásquez R, Rios N, Cubilla-Rios L. Exploring the Antibacterial Activity of Pestalotiopsis spp. under Different Culture Conditions and Their Chemical Diversity Using LC–ESI–Q–TOF–MS. Journal of Fungi. 2020; 6(3):140. https://doi.org/10.3390/jof6030140

Chicago/Turabian Style

Aguilar-Pérez, Madelaine M.; Torres-Mendoza, Daniel; Vásquez, Roger; Rios, Nivia; Cubilla-Rios, Luis. 2020. "Exploring the Antibacterial Activity of Pestalotiopsis spp. under Different Culture Conditions and Their Chemical Diversity Using LC–ESI–Q–TOF–MS" J. Fungi 6, no. 3: 140. https://doi.org/10.3390/jof6030140

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