Next Article in Journal
The Broad Clinical Spectrum of Disseminated Histoplasmosis in HIV-Infected Patients: A 30 Years’ Experience in French Guiana
Previous Article in Journal
Serial Detection of Circulating Mucorales DNA in Invasive Mucormycosis: A Retrospective Multicenter Evaluation
Open AccessCommunication

The Fungal Cell Death Regulator czt-1 Is Allelic to acr-3

1
ICBAS-Instituto de Ciências Biomédicas Abel Salazar, University of Porto, Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal
2
Fungal Genetics Stock Center, Department of Plant Pathology, Kansas State University, 4024 Throckmorton Plant Sciences Center, Manhattan, KS 66506, USA
3
Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA
4
Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720, USA
5
i3S—Institute for Research and Innovation in Health, University of Porto, Rua Alfredo Allen 208, 4200-135 Porto, Portugal
*
Author to whom correspondence should be addressed.
Present address: Institute of Molecular Biology, Academia Sinica, Taipei 11529, Taiwan.
Present address: Bolt Threads Inc., 5858 Horton St #400, Emeryville, CA 94608, USA.
J. Fungi 2019, 5(4), 114; https://doi.org/10.3390/jof5040114
Received: 12 November 2019 / Revised: 4 December 2019 / Accepted: 4 December 2019 / Published: 6 December 2019
Fungal infections have far-reaching implications that range from severe human disease to a panoply of disruptive agricultural and ecological effects, making it imperative to identify and understand the molecular pathways governing the response to antifungal compounds. In this context, CZT-1 (cell death-activated zinc cluster transcription factor) functions as a master regulator of cell death and drug susceptibility in Neurospora crassa. Here we provide evidence indicating that czt-1 is allelic to acr-3, a previously described locus that we now found to harbor a point mutation in its coding sequence. This nonsynonymous amino acid substitution in a low complexity region of CZT-1/ACR-3 caused a robust gain-of-function that led to reduced sensitivity to acriflavine and staurosporine, and increased expression of the drug efflux pump abc-3. Thus, accumulating evidence shows that CZT-1 is an important broad regulator of the cellular response to various antifungal compounds that appear to share common molecular targets. View Full-Text
Keywords: antimicrobial drug resistance; cell death; CZT-1; ABC-3; acriflavine; staurosporine antimicrobial drug resistance; cell death; CZT-1; ABC-3; acriflavine; staurosporine
Show Figures

Figure 1

MDPI and ACS Style

Gonçalves, A.P.; McCluskey, K.; Glass, N.L.; Videira, A. The Fungal Cell Death Regulator czt-1 Is Allelic to acr-3. J. Fungi 2019, 5, 114.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop