Aspergillus nidulans gfdB, Encoding the Hyperosmotic Stress Protein Glycerol-3-phosphate Dehydrogenase, Disrupts Osmoadaptation in Aspergillus wentii
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains and Culture Conditions
2.2. Reverse-Transcription Quantitative Real-Time Polymerase Chain Reaction (RT-qPCR) Assays
2.3. High Throughput RNA Sequencing
2.4. Evaluation of the Transcriptome Data
3. Results
3.1. Deletion of gfdB in A. nidulans Produced Negligible Effect on Osmotolerance, While Expression of A. nidulans gfdB in A. wentii Reduced Growth, Irrespectively of the Osmolarity
3.2. RNAseq Data Revealed That the Absence of GfdB Caused Only Minor Changes in the Osmotic Stress Response of A. nidulans
3.3. Expression of A. nidulans gfdB Substantially Disturbed the Physiology of A. wentii
4. Discussion
4.1. A. nidulans THS30 Showed Canonic Hyperosmotic Stress Response under Sorbitol or NaCl Stress
4.2. The Combination of Sorbitol and NaCl Stress Treatments Was Antagonistic
4.3. The Responses of A. wentii CBS141173 to Sorbitol and NaCl Were Not Canonic Hyperosmotic Stress Responses
4.4. GfdB–Easier to Lose Than to Get Back
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Strain | Genotype | Reference |
---|---|---|
A. nidulans THS30 | pyrG89, AfupyrG+; pyroA+; veA+ | [32] |
A. nidulans ΔgfdB | pyrG89; ΔgfdB::AfupyrG+; pyroA+; veA+ | [24] |
A. wentii CBS141173 | Wild-type | [22] |
A. wentii ′c gfdB | CBS141173 strain harboring one copy of A. nidulans gfdB with its own promoter and terminator sequences and HygR as a selection marker gene | [28] |
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Bodnár, V.; Antal, K.; de Vries, R.P.; Pócsi, I.; Emri, T. Aspergillus nidulans gfdB, Encoding the Hyperosmotic Stress Protein Glycerol-3-phosphate Dehydrogenase, Disrupts Osmoadaptation in Aspergillus wentii. J. Fungi 2024, 10, 291. https://doi.org/10.3390/jof10040291
Bodnár V, Antal K, de Vries RP, Pócsi I, Emri T. Aspergillus nidulans gfdB, Encoding the Hyperosmotic Stress Protein Glycerol-3-phosphate Dehydrogenase, Disrupts Osmoadaptation in Aspergillus wentii. Journal of Fungi. 2024; 10(4):291. https://doi.org/10.3390/jof10040291
Chicago/Turabian StyleBodnár, Veronika, Károly Antal, Ronald P. de Vries, István Pócsi, and Tamás Emri. 2024. "Aspergillus nidulans gfdB, Encoding the Hyperosmotic Stress Protein Glycerol-3-phosphate Dehydrogenase, Disrupts Osmoadaptation in Aspergillus wentii" Journal of Fungi 10, no. 4: 291. https://doi.org/10.3390/jof10040291