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From the third issue of 2018, Sinusitis has changed its name to Sinusitis and Asthma.

Open AccessArticle
Sinusitis 2016, 1(1), 13-23;

The Nasal Nitric Oxide Response to External Acoustic Energy: A Pilot Study of Sampling Dynamics

Upper Airway Biology Laboratory, Division of Occupational and Environmental Medicine, University of California San Francisco, 1301 So. 46th Street, Building 112, Room 6, Richmond, CA 94804, USA
Academic Editor: Isam Alobid
Received: 9 November 2015 / Revised: 24 November 2015 / Accepted: 24 November 2015 / Published: 27 November 2015
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Background: The paranasal sinuses serve as a reservoir of nitric oxide (NO), contributing to baseline nasal NO (nNO) levels. nNO has also been shown to increase transiently with humming, a response that may be blunted in severe rhinosinusitis. Blunting of the acoustically-induced nNO transient (“spike”) has been proposed as a screening test for osteomeatal complex (OMC) obstruction in sinusitis. Preparatory to conducting a clinical evaluation study, to eliminate variation in patient effort during this maneuver, we evaluated the use of external acoustic energy—in place of humming—to elicit nNO transients, documenting the effects of varying stimulus amplitude and gas sampling rates. Methods: Non-smoking, non-asthmatic subjects with no history of chronic sinusitis or nasal polyposis underwent nNO measurements in triplicate under: (1) control (quiet) conditions, and (2) with 128 Hz external acoustic energy. In Experiment 1, twelve subjects were exposed to two different intensities of external acoustic energy at 3 L/min sampling rate. In Experiment 2, a subset of nine subjects was sampled with and without acoustic stimulation at three different gas sampling rates (1, 2, and 3 L/min). Results: Experiment 1: Subjects, as a group, showed intensity-related increases in nNO with increasing acoustic amplitude (p < 0.01). Experiment 2: independently, both applied acoustic energy and lower nasal gas sampling rates increased measured nNO levels (p < 0.05 to p < 0.0001). Longitudinally, baseline (quiet) nNO obtained on a repeated basis in the two experiments (n = 9) was highly reproducible (R2 = 0.84; p < 0.001), and acoustically-stimulated nNO was moderately so (R2 = 0.50; p < 0.05). Conclusions: Application of external acoustic energy is a practical alternative to humming for mobilizing NO from the paranasal sinuses, and could be more objectively applied in any future validation studies involving clinical sinusitis and/or OMC obstruction. View Full-Text
Keywords: paranasal sinuses; nasal cavity; nitric oxide; osteomeatal complex; acoustic stimulation paranasal sinuses; nasal cavity; nitric oxide; osteomeatal complex; acoustic stimulation

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Shusterman, D. The Nasal Nitric Oxide Response to External Acoustic Energy: A Pilot Study of Sampling Dynamics. Sinusitis 2016, 1, 13-23.

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