Electrocardiographic Characteristics and Their Correlation with Echocardiographic Alterations in Fabry Disease
by
, , and
Matthew Zada
1,2
,
Queenie Lo
3,
Siddharth J. Trivedi
1,2,
Mehmet Harapoz
1,2,
Anita C. Boyd
4,
Kerry Devine
5,
Norman Sadick
1,2,
Michel C. Tchan
5 and
Liza Thomas
1,2,3,* 1
Westmead Clinical School, Westmead Hospital, University of Sydney, Sydney 2145, Australia
2
Department of Cardiology, Westmead Hospital, Sydney 2145, Australia
3
South Western Sydney Clinical School, Liverpool Hospital, University of New South Wales, Sydney 1466, Australia
4
Westmead Private Cardiology, Sydney 2145, Australia
5
Genetic Medicine, Westmead Hospital, Sydney 2145, Australia
*
Author to whom correspondence should be addressed.
J. Cardiovasc. Dev. Dis. 2022, 9(1), 11; https://doi.org/10.3390/jcdd9010011
Submission received: 18 November 2021
/
Revised: 23 December 2021
/
Accepted: 31 December 2021
/
Published: 3 January 2022
Abstract
:Fabry disease (FD) is an X-linked disorder with α-galactosidase A deficiency. Males (>30 years) and females (>40 years) often present with cardiac manifestations, predominantly left ventricular hypertrophy (LVH). The aim of this study was to evaluate electrocardiographic (ECG) characteristics within FD patients to identify gender related differences, and to additionally explore the association of ECG parameters with structural and functional alterations on transthoracic echocardiography (TTE). Retrospective cross-sectional analysis of 45 FD patients with contemporaneous ECG and TTE was performed and compared to age and gender matched healthy controls. FD patients demonstrated alterations in several ECG parameters particularly in males, including prolonged P-wave duration (91 vs. 81 ms, p = 0.022), prolonged QRS duration (96 vs. 84 ms, p < 0.001), increased R-wave amplitude in lead I (8.1 vs. 5.7 mV, p = 0.047), increased Sokolow–Lyon index (25 vs. 19 mV, p = 0.002) and were more likely to meet LVH criteria (31% vs. 7%, p = 0.006). FD patients with impaired basal longitudinal strain (LS) on TTE were more likely to meet LVH criteria (41% vs. 0%, p = 0.018). Those with more advanced FD (increased LV wall thickness on TTE) were more likely to meet LVH criteria but additionally demonstrated prolonged ventricular depolarization (QRS duration 101 vs. 88 ms, p = 0.044). Therefore, alterations on ECG demonstrating delayed atrial activation, delayed ventricular depolarization and evidence of LVH were more often seen in male FD patients. Impaired basal LS, a TTE marker of early cardiac involvement, correlated with ECG abnormalities. Increased LV wall thickness on TTE, a marker of more advanced FD, was associated with more severe ECG abnormalities.
1. Introduction
Fabry disease (FD) is an X-linked lysosomal storage disorder due to α-galactosidase A enzyme deficiency, with resultant progressive accumulation of globotriaosylceramide (Gb3) in various organ tissues [1]. There are a variety of clinical manifestations including neurological, gastrointestinal, renal and cardiac in affected individuals [2]. Whilst many symptoms occur in childhood, cardiac symptoms usually do not present until the third or fourth decade [3].
FD patients with cardiac involvement develop several manifestations, typically with progressive increase in left ventricular (LV) wall thickness (on transthoracic echocardiography (TTE) or on cardiac magnetic resonance imaging (CMR)), significant life-threatening arrythmias or conduction defects, interstitial remodeling and myocardial fibrosis (as demonstrated on myocardial biopsy) [4]. Electrocardiographic (ECG) alterations have been reported including altered conduction, demonstrated by a prolonged PQ interval and QRS duration, as well as ECG features of LVH [5,6,7]. Prior studies of FD patients have shown a correlation between prolonged QRS duration on ECG and LV mass on TTE [8] and CMR [9]. Early diagnosis of FD is imperative as the evidence suggests that LVH may be prevented or may regress with early treatment with enzyme replacement therapy (ERT); moreover, in advanced FD, the response to ERT is poor [10]. Recently, there has been interest in identifying ECG parameters associated with cardiac involvement prior to the development of LVH [11,12].
As FD is an X-linked condition, traditionally female heterozygotes were considered carriers [13]; however, it is now widely accepted that female FD patients can develop cardiomyopathy, but with delayed onset and slower progression [14]. Variable findings have been reported in relation to ECG changes in females with FD [6].
In this retrospective cross-sectional study, our aim was to characterize ECG parameters in FD patients compared to healthy controls and evaluate gender related differences in ECG parameters. We further sought to examine the relationship between two widely available investigations and evaluate the correlation between ECG parameters with structural (e.g., LV mass) and functional changes (e.g., longitudinal strain) on TTE. In particular, we examined the relationship of ECG parameters with basal longitudinal strain, a marker of early cardiac involvement [15].
2. Materials and Methods
We retrospectively analyzed data from 45 genetically confirmed FD patients (mean age 42 yrs, 26/45 males) from the Department of Genetic Medicine at Westmead Hospital between 2009–2014 (baseline characteristics in Supplementary Table S2). The majority (38) of patients had contemporaneous TTE and ECG performed prior to ERT therapy; whilst only 7 patients had already been commenced on ERT at the time of their TTE and ECG. Subsequently, a further 19 FD patients received ERT based on current guidelines in Australia. FD patients were compared to age and gender matched healthy controls from a departmental database, with no documented cardiovascular disease or risk factors and were not on any medications. Ethical approval was obtained from the South West Sydney Health District Human Research Ethics Committee.
2.1. Electrocardiography
Standard 12-lead ECGs were performed, recorded at 50 mm/s with an amplitude of 1 mV/10 mm. An average of 3 measurements were performed manually with the aid of calipers for all parameters. Figure 1 demonstrates the ECG parameters that were assessed including PQ interval (average of PQ interval in lead II and V1), P-wave duration (average of P-wave duration in lead II and V1), QRS duration (in lead II), R wave amplitude in lead I, Sokolow–Lyon index (sum of voltage of S wave in V1/V2 and R wave in V5/V6) using the previously described criteria and the Modified Cornell Index (measuring R wave amplitude in aVL with patients meeting LVH criteria if >12 mm) [16]. PQ interval and P-wave duration were corrected to a heart rate of 60 by dividing intervals by the heart rate and multiplying by 60. ECGs were assessed for meeting LVH (Sokolow–Lyon criteria) and right bundle branch block (RBBB). ECG parameters were compared between the following groups: FD patients vs. controls (Table 1A); male FD patients vs. male controls (Table 1A); female FD patients vs. female controls (Table 1A); and male FD patients vs. female FD patients (Table 1B). We examined ECG findings in subgroups of FD patients based on TTE criteria: increased LV wall thickness vs. normal LV wall thickness, impaired GLS vs. normal GLS and impaired basal longitudinal strain (LS) vs. normal basal LS (Supplementary Table S1).
2.2. Echocardiography
A comprehensive TTE was performed using commercially available ultrasound systems (Vivid 7 or E9 ultrasound scanner; GE Healthcare, Horten, Norway) with a 2.5-MHz transducer; M-mode, 2D, color, Doppler and tissue Doppler imaging were obtained in accordance with American Society of Echocardiography (ASE) guidelines [17]. Digital images were stored for offline analysis. One patient was excluded from analysis due to poor image quality. An average of 3 measurements were used for all echocardiographic parameters. LV wall thickness was measured from 2D images from the parasternal long axis view in end diastole; FD patients were stratified based on ASE guidelines to have increased wall thickness if ≥11 mm for males and ≥10 mm for females [18]. LV mass was calculated using the Devereux method as per ASE criteria and indexed (LVMI) to body surface area (BSA) [19]; gender-based cutoffs for increased LV mass used were >115 g/m2 for males and >95 g/m2 for females. LVEF was calculated using Simpson’s biplane method by measuring LV end diastolic and end systolic volumes from apical−4 and −2 chamber views. Biplane maximum left atrial volume at end systole was measured from the apical−4 and −2 chamber views and indexed to body surface area (LAVI). Peak E and peak A velocities were obtained from pulsed Doppler mitral inflow. Tissue Doppler velocities (e’) were measured from the septal and lateral mitral annulus. The ratio of E velocity over average of septal and lateral e’ velocity was determined (E/e’). (Table 2).
2.3. Strain Analysis
2D global longitudinal strain (GLS) analysis was performed using customized offline computer software (EchoPAC, GE, Horten, Norway, Version 201), from zoomed apical LV 4-, 2- and 3-chamber views, obtained at high frame rates (>55 fps). The endocardial border was manually traced in end-systole and the width of the region of interest (ROI) was manually adjusted to track the myocardium. Strain from up to 2 segments could be excluded in one imaging plane; if >2 segments could not be adequately tracked, the strain measurement from this plane was omitted. Impaired GLS was defined as GLS worse than −18.0% [20] and impaired basal longitudinal strain (LS) was defined as basal LS worse than −18.1% [15].
2.4. Inter-Observer and Intra-Observer Variability
To assess intra-observer variability, TTE and ECG of 10 randomly selected patients were reviewed by the same investigator 4 weeks apart. Inter-observer variability was assessed by a second blinded investigator in the same 10 patients.
2.5. Statistical Analysis
Continuous variables are expressed as a mean, and categorical variables as a percentage. Non-parametric tests (Mann-Whitney) were performed to examine differences in means between FD patients and controls and between male and female FD patients. Chi-square analysis was performed to examine differences in categorical variables. Pearsons correlation was performed to evaluate associations between continuous variables. Statistical analyses were performed using Statistical Package for Social Sciences, version 22 (SPSS, Chicago, IL, USA) and MedCalc version 15.8 (MedCalc Software Ltd., Ostend, Belgium) with p < 0.05 considered statistically significant. Inter and intra-observer variability were evaluated using intra-class correlation (ICC) and the coefficient of variation (CoV).
3. Results
3.1. ECG in FD versus Controls
All FD patients were in sinus rhythm. Analysis by gender (Table 1A) showed that corrected PQ interval was longer in male FD patients (169 vs. 148 ms, p = 0.027) but not so for female FD patients (126 vs. 121 ms, p = 0.729). The corrected P-wave duration was longer in FD patients (91 vs. 81 ms, p < 0.022), but similar to the corrected PQ interval, was significantly longer for male FD patients (105 vs. 88 ms, p = 0.008), but not so in female FD patients (74 vs. 72 ms, p = 0.644). The QRS duration was significantly longer in FD patients compared to controls (96 vs. 84 ms, p < 0.001) and again, QRS duration was significantly longer in male FD patients (103 vs. 85 ms, p < 0.001), but not so in female FD patients (87 vs. 82 ms, p = 0.271).
Amplitude of the QRS complex was higher in FD patients in lead I (8.1 vs. 5.7 mV, p = 0.047) and in the chest leads (25 vs. 19 mV, p < 0.002). When analyzed by gender, QRS amplitude was higher in male FD patients both in lead I (9.1 vs. 5.8 mV, p= 0.014) and the chest leads (28.4 vs. 19.6 mV, p = 0.008), but not for female FD patients (lead I: 6.7 vs. 5.1 mV, p = 0.885; chest leads: 21.5 vs. 17.7 mV, p = 0.065). FD patients were more likely to meet Sokolow–Lyon criteria for LVH (31% vs. 7%, p = 0.006) and male FD patients met LVH criteria more frequently versus controls (42% vs. 8%, p = 0.009), though not observed in female FD patients (16% vs. 5%, p = 0.604). There was no significant difference in the presence of RBBB in FD patients compared to controls (11% vs. 4%, p = 0.434).
3.2. Male FD vs. Female FD: ECG and Echocardiographic Correlates
The corrected PQ interval, P wave duration and QRS duration were significantly longer in male FD patients compared to female FD patients (Table 1B). The average voltage was higher in male FD patients compared to female FD patients in the chest leads (28.4 vs. 21.5 mV, p = 0.043) but not in lead I (9.1 vs. 6.7 mV, p = 0.051). Male FD patients met LVH criteria more often than females, though not statistically significant (42% vs. 16%, p = 0.102). Only male FD patients had RBBB when compared to females (5/26 vs. 0/19). Male FD patients were more likely to have increased LV wall thickness on TTE (77% vs. 37%, p = 0.013). Strain analysis demonstrated that male and female FD patients were as likely to have impaired GLS (69% vs. 55%, p = 0.525) and impaired basal LS (89% vs. 61%, p = 0.064).
3.3. Correlation of Electrocardiographic and Echocardiographic Markers
There was a significant correlation (Figure 2) between LAVI and the corrected PQ interval (r = 0.42, p = 0.005) as well as the corrected P-wave duration (r = 0.48, p = 0.003).
There was a significant correlation (Figure 2) between LVMI on TTE with QRS duration (r = 0.30, p = 0.044) and with Sokolow–Lyon index on ECG (r = 0.39, p = 0.011). Similarly, there were significant correlations between GLS and QRS duration (r = 0.47, p = 0.001) and Sokolow–Lyon index (r = 0.54, p < 0.001), as also between basal LS and QRS duration (r = 0.59, p < 0.001) and Sokolow–Lyon index (r = 0.56, p = 0.004).
3.4. ECG Characteristics in FD Patients with Altered TTE Parameters: Increased Wall Thickness, Altered GLS and Basal Segmental LS
Subgroup analysis of FD patients (Supplementary Table S1) showed that those with increased wall thickness on TTE (n = 27) had a significantly longer QRS duration (101 vs. 88 ms, p = 0.044) and higher QRS complex amplitude in lead I (9.9 vs. 5.4 mV, p = 0.002) but not so in the chest leads (27.7 vs. 21.8 mV, p = 0.112). As expected, FD patients with increased wall thickness on TTE were also more likely to meet LVH criteria on ECG (48% vs. 6%, p = 0.003).
FD patients with impaired GLS (n = 28) had higher QRS complex amplitude in lead I (9.7 vs. 5.7 mV, p = 0.008) and were more likely to meet LVH criteria on ECG (43% vs. 13%, p = 0.049). Similarly, FD patients with impaired basal LS (n = 34) had higher QRS complex amplitude in lead I (9.1 vs. 5.1 mV, p = 0.014) and were also more likely to meet LVH criteria on ECG (41% vs. 0%, p = 0.018).
We examined ‘stages’ of disease progression by dividing the FD patients into three groups: (1) EARLY (Group A): normal LV wall thickness and normal basal segmental LS; (2) INTERMEDIATE (Group B): normal LV wall thickness and impaired basal segmental LS; and (3) LATE (Group C): increased LV wall thickness and impaired basal LS. There were significant differences in ECG and TTE parameters (Table 2), albeit acknowledging the relatively small numbers in groups 1 and 2. Two female FD patients with hypertension and mildly increased LV wall thickness (likely due to hypertension) with normal basal LS were excluded from this subgroup analysis.
3.5. Reproducibility of ECG Analysis
The ICC for inter-observer variability for the PQ interval was 0.90 (95% confidence interval [CI], 0.62–0.98; coefficient of variation (COV), 6.22%) whilst the intraobserver variability for the PQ interval was 0.92 (95% CI, 0.68–0.98; COV = 5.47%). The ICC for inter-observer variability for the QRS duration was 0.86 (95% CI, 0.45–0.97; COV = 6.32%) whilst the intraobserver variability for the QRS duration was 0.84 (95% CI 0.35–0.96; COV = 5.65%). The ICC for inter-observer variability for LVH Sokolow–Lyon index was 0.97 (95% CI 0.89–0.99; COV = 7.87%) whilst the intraobserver variability for LVH Sokolow–Lyon index was 0.99 (95% CI 0.95–0.99; COV = 5.31%).
We have previously reported inter- and intra-observer variability for TTE parameters GLS and segmental strain [15].
4. Discussion
In this single center cohort of FD patients, males more commonly had alteration in ECG parameters including prolonged corrected PQ interval (i.e., lengthened P-wave duration), prolonged QRS duration and evidence of LVH on ECG. These ECG alterations were not observed as frequently in female FD patients. Prolonged QRS duration and Sokolow–Lyon index correlated with traditional TTE abnormalities (increased LV wall thickness) but had a stronger correlation with novel TTE markers such as GLS and basal LS.
4.1. ECG Parameters in FD Males and Females
Our results support previous observations that significant ECG alterations are more likely to be present in FD males. Despite one study not finding significant differences in the PQ interval and QRS interval between FD males and females [6], the study of Niemann et al. evaluating 150 FD patients (63 males and 87 females; average age of 38 years), demonstrated that FD males were more likely to have prolonged QRS duration, higher Sokolow–Lyon index and RBBB criteria [9]. Additionally, we identified altered PQ interval, P wave duration and QRS duration in FD males. In general, in FD, cardiac involvement occurs earlier and more commonly in males [14]. Therefore, our observations suggest that FD males, albeit at similar or younger age than female FD patients were at a later stage in the disease course, with more males having increased wall thickness on TTE and more ECG alterations.
4.2. Alterations in Conduction: PQ Interval and P-Wave Duration
Interestingly, young patients with early-stage FD have been reported to have PQ interval shortening, as a result of P wave shortening [5,6], without evidence of an accessory pathway [21]; however, none of the patients in our study demonstrated this. Potential mechanisms for enhanced conduction velocity include accumulation of Gb3 in cardiomyocytes, increase in diameter of conducting cells and direct interaction between Gb3 and ion channels involved in action potential propagation [5,6]. In other glycogen storage disorders, it is thought that electrophysiological phenomena such as PQ interval shortening are directly related to glycogen storage in and around the atrioventricular node [22]. ERT normalizes the shortened PQ intervals and shortened P waves [23]. As FD progresses, the extent of infiltration worsens with macroscopic changes to the myocardium, causing PQ interval prolongation (corresponding to left atrial enlargement) [5]. This theory is supported by the correlation of LAVI to PQ interval in our cohort.
4.3. Alterations in Conduction: QRS Interval and Right Bundle Branch Block
Patients in early-stage FD have QRS interval shortening, likely a result of enhanced conduction velocity [5,6]. With disease progression, the QRS interval prolongs [7]. In patients with LVH (from any cause), prolonged QRS interval is often seen and is thought to be due to the increased time taken for left ventricular depolarization [24,25,26]. As previously shown by Kampmann et al. [8], our cohort also demonstrated prolonged QRS duration on ECG, likely secondary to increased LV mass, considering almost two-thirds of our cohort demonstrated increased LV wall thickness on TTE (27/45).
Similar to previous studies, our cohort demonstrated right bundle branch block more commonly than left bundle branch block [9,27,28]. We hypothesize that accumulation of Gb3 occurs more often within the right bundle than within the left bundle in patients with FD. There is certainly histological evidence reported in the literature of vacuolization within myocytes with preferential extension into the right bundle [29].
4.4. ECG for Detecting LVH (Sokolow–Lyon Criteria)
With worsening cardiomyopathy in FD, LV wall thickness increases with an increased Sokolow–Lyon index on ECG [7]. FD males in our study showed evidence of LV hypertrophy with several meeting the Sokolow–Lyon criteria on ECG. This is largely attributable to male FD patients being at ‘a later stage’ of their disease, evidenced by 20/26 male FD patients having increased LV wall thickness on TTE.
Although 27/45 patients had increased LV wall thickness on TTE, only 13/27 (48%) met Sokolow–Lyon criteria for LVH on ECG. This is in keeping with previous studies that have shown that the sensitivity of ECG at detecting LVH varies depending on the method used but ranges from 20–55%, with specificity decreasing from 95% to 77% as sensitivity increases [25,26,30]. Therefore, even though ECG is a simple method of screening for LV hypertrophy in FD, it is not as sensitive as TTE.
4.5. Correlating Electrocardiographic and Echocardiographic Parameters
Namdar et al. have reported that PQ interval may be prolonged in FD due to left atrial enlargement [5]. We demonstrated a significant correlation between LAVI and both PQ interval and P-wave duration, supporting the concept that the PQ interval is prolonged due to delayed atrial depolarization secondary to left atrial enlargement.
Kampmann et al. analyzed 166 ECGs and TTEs of hemi- and heterozygous FD patients (44% males; 56% females; average age 35 years) and found a significant correlation between Sokolow–Lyon index and QRS duration with LVMI [8], similar to our findings. GLS identifies subclinical cardiac involvement in a variety of conditions [31]; and in FD, GLS can be impaired even with normal LV wall thickness [15]. Augosto et al. have recently described several phases of FD including a silent ‘accumulation stage’ where LV wall thickness is normal, with evidence of detectable infiltration on CMR with corresponding impairment in GLS on TTE [32]. A previous study that used a binary definition to classify patients into 2 groups—normal versus abnormal ECG (defined as any of the following: prolonged or shortened PQ interval; prolonged QRS duration; the presence of LVH criteria; or t wave inversion), also demonstrated correlation with impaired GLS [33].
We sought to further explore the relationship between ECG parameters and strain parameters. In our cohort of 45 FD patients, 28 (62%) had impaired GLS and 34 (76%) had impaired basal LS; whilst 27 (60%) had increased LV wall thickness on TTE (Supplementary Table S1). This would suggest that strain, in particular basal LS, is more sensitive for detecting cardiac involvement in FD (with strain impaired in FD patients with normal LV wall thickness). FD patients with impaired basal LS demonstrated ECG abnormalities such as Sokolow–Lyon LVH criteria, but not QRS prolongation. Whereas patients with increased LV wall thickness on TTE, represent a group with more advanced disease and more severe ECG abnormalities (Sokolow–Lyon LVH criteria as well as QRS prolongation).
To our knowledge, this is the first instance where ECG abnormalities have been correlated to impaired basal LS in FD patients. This has practical implications for patient screening; if for example there is evidence of LVH on an ECG, but the LV wall thickness is normal on TTE, this may prompt the clinician to investigate for cardiac involvement by assessing basal longitudinal strain. Other investigations including cardiac MRI or myocardial biopsy may be confirmatory.
Therefore, we have proposed a model which highlights these findings and demonstrates corresponding ECG and TTE findings that could be expected with disease progression in FD (Figure 3): In early-stage FD, LV wall thickness, GLS and basal LS are normal—ECG characteristics may include shortened PQ interval and QRS duration due to enhanced conduction (although this was not seen in our cohort probably due to most of our cohort being at a later stage in the disease process); In intermediate stage FD, LV wall thickness is normal although there may be evidence of impairment in longitudinal strain, particularly basal LS—in such patients the PQ interval and QRS duration lengthen so that they lie within the normal range and the Sokolow–Lyon index may be increased; In late-stage FD, LV wall thickness is increased and GLS as well as basal LS are impaired—ECG characteristics would include evidence of LVH (increased Sokolow–Lyon index) and prolongation of the PQ interval and QRS duration (with a predilection for development of RBBB).
4.6. Limitations
This study had a modest sample size; however, it is inherently difficult to recruit large numbers of FD patients at a single center, given the low disease prevalence. Despite this we were able to demonstrate differences in ECG characteristics in FD patients.
CMR was not performed at a contemporaneous time as the ECG and TTE. Hence correlation with CMR findings could not be reported meaningfully. 3D echocardiography that allows more accurate estimation of LV mass and cardiac volumes was not performed as several patients had TTE performed prior to 3D technology being widely available.
Hypertensive heart disease may mimic some of the structural changes observed in FD. 60% of FD patients had a history of hypertension; however, all were managed according to guidelines and blood pressure was well controlled except for 1 female FD patient who was hypertensive at the time of assessment (with a systolic blood pressure more than 140 mmHg). Hence it is likely that changes observed are more attributable to FD than coexistent hypertension.
Our study compared FD patients to healthy controls as opposed to those with hypertrophic phenotype (e.g., hypertensive heart disease). However, a previous publication [16] has reported ECG characteristics in varying types of hypertrophic phenotypes.
5. Conclusions
ECG abnormalities in FD patients are more common in male FD patients. Alterations in conduction present in FD include prolonged PQ interval, prolonged P-wave duration and prolonged QRS duration (particularly RBBB). Impaired longitudinal strain on TTE is an earlier marker of cardiac involvement in FD and is associated with LVH on ECG. However, increased LV wall thickness on TTE occurs at a later stage in the disease process and in such instances demonstrates LVH and prolonged ventricular depolarization on ECG.
Supplementary Materials
The following are available online at https://www.mdpi.com/article/10.3390/jcdd9010011/s1, Table S1: Electrocardiographic characteristics of FD patients in comparison to echocardiographic findings. ECG parameters assessed are listed in the first column. Comparison is made between FD patients with increased LV wall thickness and normal LV wall thickness; FD patients with impaired GLS vs. normal GLS; and between FD with impaired basal LS and normal basal LS, Table S2: Baseline clinical and echocardiographic characteristics of FD patients. Table S3. Comparison of clinical, echocardiographic and electrocardiographic markers of FD patients with normal LV wall thickness (cut off 13mm). and normal basal LS; normal LV wall thickness with impaired basal LS; and increased LV wall thickness and impaired basal LS.
Author Contributions
Conceptualization, L.T.; Data curation, M.Z., Q.L., S.J.T., A.C.B., K.D., N.S., M.C.T. and L.T.; Formal analysis, M.Z. and Q.L.; Funding acquisition, L.T.; Investigation, M.Z. and Q.L.; Methodology, M.Z. and L.T.; Project administration, L.T.; Resources, S.J.T.; Supervision, L.T.; Validation, M.Z. and M.H.; Visualization, M.Z.; Writing—original draft, M.Z.; Writing—review & editing, M.C.T. and L.T. All authors have read and agreed to the published version of the manuscript.
Funding
Matthew Zada was supported by the Jerry Koutts fellowship for a Masters Research Program. Siddharth J Trivedi was supported by the Cardiac Society of Australia and New Zealand Research Scholarship (RS2/18) and the National Health and Medical Research Council Postgraduate Scholarship (1168854).
Institutional Review Board Statement
The study was conducted according to the guidelines of the Declaration of Helsinki, and approved by the South West Sydney Health District Human Research Ethics Committee (Approval number ETH13628).
Informed Consent Statement
Patient consent was waived due to retrospective nature of this study. All patient information has been deidentified.
Conflicts of Interest
The authors declare no conflict of interest.
References
- Garman, S.C.; Garboczi, D.N. The molecular defect leading to Fabry disease: Structure of human alpha-galactosidase. J. Mol. Biol. 2004, 337, 319–335. [Google Scholar] [CrossRef]
- Favalli, V.; Disabella, E.; Molinaro, M. Genetic Screening of Anderson-Fabry Disease in Probands Referred From Multispecialty Clinics. J. Am. Coll Cardiol. 2016, 68, 1037–1050. [Google Scholar] [CrossRef]
- Linhart, A.; Elliott, P.M. The heart in Anderson-Fabry disease and other lysosomal storage disorders. Heart. 2007, 93, 528–535. [Google Scholar] [CrossRef] [Green Version]
- Weidemann, F.; Breunig, F.; Beer, M. The variation of morphological and functional cardiac manifestation in Fabry disease: Potential implications for the time course of the disease. Eur. Heart J. 2005, 26, 1221–1227. [Google Scholar] [CrossRef]
- Namdar, M. Electrocardiographic Changes and Arrhythmia in Fabry Disease. Front. Cardiovasc. Med. 2016, 3, 7. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Namdar, M.; Kampmann, C.; Steffel, J. PQ interval in patients with Fabry disease. Am. J. Cardiol. 2010, 105, 753–756. [Google Scholar] [CrossRef] [PubMed]
- Marafioti, V. The electrocardiographic features of Fabry disease and amyloidosis. Am. J. Emerg. Med. 2006, 24, 640. [Google Scholar] [CrossRef] [PubMed]
- Kampmann, C.; Wiethoff, C.M.; Martin, C. Electrocardiographic signs of hypertrophy in fabry disease-associated hypertrophic cardiomyopathy. Acta Paediat. Suppl. 2002, 91, 21–27. [Google Scholar] [CrossRef]
- Niemann, M.; Hartmann, T.; Namdar, M. Cross-sectional baseline analysis of electrocardiography in a large cohort of patients with untreated Fabry disease. J. Inherit. Metabol. Dis. 2013, 36, 873–879. [Google Scholar] [CrossRef]
- Pieroni, M.; Moon, J.C.; Arbustini, E. Cardiac Involvement in Fabry Disease: JACC Review Topic of the Week. J. Am. Coll Cardiol. 2021, 77, 922–936. [Google Scholar] [CrossRef] [PubMed]
- Namdar, M.; Richardot, P.; Johner, N. Recognition of pre-hypertrophic cardiac involvement in Fabry Disease based on automated electrocardiographic measures. Int. J. Cardiol. 2021, 338, 121–126. [Google Scholar] [CrossRef] [PubMed]
- Figliozzi, S.; Camporeale, A.; Boveri, S. ECG-based score estimates the probability to detect Fabry Disease cardiac involvement. Int. J. Cardiol. 2021, 339, 110–117. [Google Scholar] [CrossRef] [PubMed]
- Desnick, R.I.Y.; Eng, C. Fabry Disease: Alpha Galactosidase A Deficiency; McGraw Hill: New York, NY, USA, 1995. [Google Scholar]
- Mehta, A.; Ricci, R.; Widmer, U.; Dehout, F.; de Lorenzo, A.G.; Kampmann, C.; Linhart, A.; Sunder-Plassmann, G.; Ries, M.; Beck, M. Fabry disease defined: Baseline clinical manifestations of 366 patients in the Fabry Outcome Survey. Eur. J. Clin. Investig. 2004, 34, 236–242.e402. [Google Scholar] [CrossRef]
- Zada, M.; Lo, Q.; Boyd, A.C. Basal Segmental Longitudinal Strain: A Marker of Subclinical Myocardial Involvement in Anderson-Fabry Disease. J. Am. Soc. Echocardiogr. 2021, 34, 405–413.e402. [Google Scholar] [CrossRef] [PubMed]
- Namdar, M.; Steffel, J.; Jetzer, S. Value of electrocardiogram in the differentiation of hypertensive heart disease, hypertrophic cardiomyopathy, aortic stenosis, amyloidosis, and Fabry disease. Am. J. Cardiol. 2012, 109, 587–593. [Google Scholar] [CrossRef]
- Mitchell, C.; Rahko, P.S.; Blauwet, L.A. Guidelines for Performing a Comprehensive Transthoracic Echocardiographic Examination in Adults: Recommendations from the American Society of Echocardiography. J. Am. Soc. Echocardiogr. 2019, 32, 1–64. [Google Scholar] [CrossRef]
- Marwick, T.H.; Gillebert, T.C.; Aurigemma, G. Recommendations on the use of echocardiography in adult hypertension: A report from the European Association of Cardiovascular Imaging (EACVI) and the American Society of Echocardiography (ASE). Eur. Heart J. Cardiovasc. Imag. 2015, 16, 577–605. [Google Scholar] [CrossRef] [Green Version]
- Lang, R.M.; Badano, L.P.; Mor-Avi, V. Recommendations for cardiac chamber quantification by echocardiography in adults: An update from the American Society of Echocardiography and the European Association of Cardiovascular Imaging. J. Am. Soc. Echocardiogr. 2015, 28, 1–39.e14. [Google Scholar] [CrossRef] [Green Version]
- Nagata, Y.; Wu, V.C.; Otsuji, Y.; Takeuchi, M. Normal range of myocardial layer-specific strain using two-dimensional speckle tracking echocardiography. PLoS ONE 2017, 12, e0180584. [Google Scholar] [CrossRef] [PubMed]
- Linhart, A.; Germain, D.P.; Olivotto, I. An expert consensus document on the management of cardiovascular manifestations of Fabry disease. Eur. J. Heart Fail. 2020, 22, 1076–1096. [Google Scholar] [CrossRef]
- Wolf, C.M.; Arad, M.; Ahmad, F. Reversibility of PRKAG2 Glycogen-Storage Cardiomyopathy and Electrophysiological Manifestations. Circulation 2008, 117, 144–154. [Google Scholar] [CrossRef] [Green Version]
- Motwani, M.; Banypersad, S.; Woolfson, P.; Waldek, S. Enzyme replacement therapy improves cardiac features and severity of Fabry disease. Mol. Genet. Metabol. 2012, 107, 197–202. [Google Scholar] [CrossRef] [PubMed]
- Kansal, S.; Roitman, D.I.; Sheffield, L.T. A quantitative relationship of electrocardiographic criteria of left ventricular hypertrophy with echocardiographic left ventricular mass: A multivariate approach. Clin. Cardiol. 1983, 6, 456–463. [Google Scholar] [CrossRef]
- Romhilt, D.W.; Estes, E.H., Jr. A point-score system for the ECG diagnosis of left ventricular hypertrophy. Am. Heart J. 1968, 75, 752–758. [Google Scholar] [CrossRef]
- Braunstein, E.D.; Croft, L.B.; Halperin, J.L.; Liao, S.L. Improved scoring system for the electrocardiographic diagnosis of left ventricular hypertrophy. World J. Cardiol. 2019, 11, 94–102. [Google Scholar] [CrossRef]
- Sadick, N.; Thomas, L. Cardiovascular manifestations in Fabry disease: A clinical and echocardiographic study. Heart Lung Circul. 2007, 16, 200–206. [Google Scholar] [CrossRef]
- Wu, J.C.; Ho, C.Y.; Skali, H. Cardiovascular manifestations of Fabry disease: Relationships between left ventricular hypertrophy, disease severity, and alpha-galactosidase A activity. Eur. Heart J. 2010, 31, 1088–1097. [Google Scholar] [CrossRef] [Green Version]
- Akhtar, M.M.; Elliott, P.M. Anderson-Fabry disease in heart failure. Biophys. Rev. 2018, 10, 1107–1119. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Bressman, M.; Mazori, A.Y.; Shulman, E. Determination of Sensitivity and Specificity of Electrocardiography for Left Ventricular Hypertrophy in a Large, Diverse Patient Population. Am. J. Med. 2020, 133, e495–e500. [Google Scholar] [CrossRef]
- Mondillo, S.; Galderisi, M.; Mele, D. Speckle-tracking echocardiography: A new technique for assessing myocardial function. J. Ultrasound. Med. 2011, 30, 71–83. [Google Scholar] [CrossRef] [PubMed]
- Augusto, J.B.; Johner, N.; Shah, D. The myocardial phenotype of Fabry disease pre-hypertrophy and pre-detectable storage. Eur. Heart J. Cardiovasc. Imag. 2021, 22, 790–799. [Google Scholar] [CrossRef] [PubMed]
- Vijapurapu, R.; Nordin, S.; Baig, S. Global longitudinal strain, myocardial storage and hypertrophy in Fabry disease. Heart 2019, 105, 470–476. [Google Scholar] [CrossRef] [PubMed]
Figure 1.
ECG parameters assessed included PQ interval in lead II and lead V1, P wave duration (PWd) in lead II and V1, QRS duration in lead II, R wave amplitude in lead 1, Sokolow–Lyon index and criteria, Modified Cornell index and criteria and complete right bundle branch block criteria.
Figure 1.
ECG parameters assessed included PQ interval in lead II and lead V1, P wave duration (PWd) in lead II and V1, QRS duration in lead II, R wave amplitude in lead 1, Sokolow–Lyon index and criteria, Modified Cornell index and criteria and complete right bundle branch block criteria.
Figure 2.
Correlation between electrocardiographic and echocardiographic parameters; (A)—Correlation between corrected PQ interval and maximum left atrial volume indexed to body surface area (LAVI) and between corrected P-wave duration and LAVI; (B)—Correlation between Sokolow–Lyon index and left ventricular mass indexed to body surface area (LVMI) and between QRS duration and LVMI; (C)—Correlation between Sokolow–Lyon index and Global Longitudinal Strain (GLS) and between QRS duration and GLS; and (D)—correlation between Sokolow–Lyon index and Basal Longitudinal Strain (LS) and between QRS duration and Basal LS.
Figure 2.
Correlation between electrocardiographic and echocardiographic parameters; (A)—Correlation between corrected PQ interval and maximum left atrial volume indexed to body surface area (LAVI) and between corrected P-wave duration and LAVI; (B)—Correlation between Sokolow–Lyon index and left ventricular mass indexed to body surface area (LVMI) and between QRS duration and LVMI; (C)—Correlation between Sokolow–Lyon index and Global Longitudinal Strain (GLS) and between QRS duration and GLS; and (D)—correlation between Sokolow–Lyon index and Basal Longitudinal Strain (LS) and between QRS duration and Basal LS.
Figure 3.
Expected transthoracic echocardiographic (TTE) and electrocardiographic (ECG) changes with progression of Fabry Disease (FD). LAVI = maximum left atrial volume indexed to body surface area. LS = longitudinal strain. GLS = global longitudinal strain. RBBB = right bundle branch criteria.
Figure 3.
Expected transthoracic echocardiographic (TTE) and electrocardiographic (ECG) changes with progression of Fabry Disease (FD). LAVI = maximum left atrial volume indexed to body surface area. LS = longitudinal strain. GLS = global longitudinal strain. RBBB = right bundle branch criteria.
Table 1.
(A). Electrocardiographic characteristics of FD patients compared to age and gender matched normal controls. ECG parameters assessed are listed in the first column. Comparison is made between FD patients and matched controls; between male FD patients and matched male controls only; and between female FD patients and matched female controls only. (B) Comparison between the electrocardiographic characteristics of male FD patients and female FD patients. ECG parameters assessed are listed in the first column.
Table 1.
(A). Electrocardiographic characteristics of FD patients compared to age and gender matched normal controls. ECG parameters assessed are listed in the first column. Comparison is made between FD patients and matched controls; between male FD patients and matched male controls only; and between female FD patients and matched female controls only. (B) Comparison between the electrocardiographic characteristics of male FD patients and female FD patients. ECG parameters assessed are listed in the first column.
| (A) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| All FD Patients Compared to Matched Controls | Male FD Patients Compared to Matched Male Controls | Female FD Patients Compared to Matched Female Controls | |||||||
| FD [n = 45] | Controls [n = 45] | p-Value | Male FD [n = 26] | Male Controls [n = 26] | p-Value | Female FD [n = 19] | Female Controls [n = 19] | p-Value | |
| Age (yrs.) | 42 | 42 | 0.939 | 42 | 42 | 0.934 | 42 | 42 | 0.84 |
| Corrected PQ Interval (ms) | 150 | 137 | 0.051 | 169 | 148 | 0.027 † | 126 | 121 | 0.729 |
| Corrected PWD (ms) | 91 | 81 | 0.022 † | 105 | 88 | 0.008 † | 74 | 72 | 0.644 |
| QRS duration (ms) | 96 | 84 | <0.001 † | 103 | 85 | <0.001 † | 87 | 82 | 0.271 |
| R wave amplitude lead I (mV) | 8.1 | 5.7 | 0.047 † | 9.1 | 5.8 | 0.014 † | 6.7 | 5.6 | 0.885 |
| Sokolow–Lyon index (mV) | 25 | 19 | 0.002 † | 28.4 | 19.6 | 0.008 † | 21.5 | 17.7 | 0.065 |
| Sokolow–Lyon LVH criteria | 14/45 (31%) | 3/45 (7%) | 0.006 † | 11/26 (42%) | 2/26 (8%) | 0.009 † | 3/19 (16%) | 1/19 (5%) | 0.604 |
| Modified Cornell Index (mV) | 4.2 | 3 | 0.507 | 5.3 | 3.2 | 0.374 | 2.7 | 2.7 | 0.965 |
| Modified Cornell LVH criteria | Apr-45 | 0/45 | 0.117 | 26-Apr | 0/26 | 0.11 | 0/19 | 0/19 | - |
| RBBB criteria | 5/45 (11%) | 2/45 (4%) | 0.434 | 5/26 (19%) | 2/26 (8%) | 0.419 | 0/19 | 0/19 | - |
| (B) | |||||||||
| ECG Parameters | Male FD [n = 26] | Female FD [n = 19] | p-value | ||||||
| Age (yrs) | 42 | 42 | 0.818 | ||||||
| Corrected PQ Interval (ms) | 169 | 126 | 0.001 † | ||||||
| Corrected PWD (ms) | 105 | 74 | <0.001 † | ||||||
| QRS duration (ms) | 103 | 87 | 0.004 † | ||||||
| R wave amplitude lead I (mV) | 9.1 | 6.7 | 0.051 | ||||||
| Sokolow–Lyon index (mV) | 28.4 | 21.5 | 0.043 † | ||||||
| Sokolow–Lyon LVH criteria | 11/26 (42%) | 3/19 (16%) | 0.102 | ||||||
| Modified Cornell Index (mV) | 5.3 | 2.7 | 0.164 | ||||||
| Modified Cornell LVH criteria | 4/26 | 0/19 | 0.126 | ||||||
| RBBB criteria | 5/26 (19%) | 0/19 | 0.063 | ||||||
| LVH on TTE | 20/26 (77%) | 7/19 (37%) | 0.013 † | ||||||
| Impaired GLS on TTE | 18/26 (69%) | 10/18 (55%) | 0.525 | ||||||
| Impaired Basal LS on TTE | 23/26 (89%%) | 11/18 (61%) | 0.064 | ||||||
Intervals and voltage are expressed as Means. Categorical variables are expressed as frequency and percentage of occurrence in each group. † p < 0.05. PWD = P-wave duration; LVH = Left Ventricular Hypertrophy; RBBB = Right bundle branch block; GLS = global longitudinal strain; LS = longitudinal strain, FD = Fabry disease, ECG = electrocardiograph
Table 2.
Comparison of clinical, echocardiographic and electrocardiographic markers of FD patients with normal LV wall thickness and normal basal LS; normal LV wall thickness with impaired basal LS; and increased LV wall thickness and impaired basal LS.
Table 2.
Comparison of clinical, echocardiographic and electrocardiographic markers of FD patients with normal LV wall thickness and normal basal LS; normal LV wall thickness with impaired basal LS; and increased LV wall thickness and impaired basal LS.
| Normal LV Wall Thickness & Normal Basal LS [n = 8] [Group A] | Normal LV Wall Thickness & Impaired Basal LS [n = 9] [Group B] | Increased LV Wall Thickness & Impaired Basal LS [n = 25] [Group C] | |
|---|---|---|---|
| Clinical characteristics | |||
| Age | 31 | 46 * | 45 |
| Males | 3 (38%) | 3 (33%) | 20 (80%) |
| ERT—no. on ERT at time of simultaneous ECG/TTE | 0 | 1 (11%) | 6 (24%) |
| ERT—total no. who were treated with ERT after ECG/TTE | 1 (13%) | 4 (44%) | 21 (84%) |
| Albuminuria/Chronic kidney disease | 0 | 1 (11%) | 12 (48%) |
| Dyslipidaemia | 1 (13%) | 5 (56%) | 15 (60%) |
| Diabetes mellitus | 0 | 2 (22%) | 1 (4%) |
| Arterial hypertension | 0 | 2 (22%) | 15 (60%) |
| Transient ischaemic attack / stroke | 1 (13%) | 2 (22%) | 5 (20%) |
| Ischaemic heart disease | 0 | 1 (11%) | 2 (8%) |
| Echocardiographic parameters | |||
| Average LV wall thickness (mm) | 8.3 | 7.5 | 14.6 + |
| Left ventricular mass index (g/m2) | 73.4 | 76.9 | 141.9 + |
| LVEF (%) | 61 | 60 | 61 |
| Peak E (cm/s) | 98 | 93 | 78 |
| Peak A (cm/s) | 63 | 64 | 66 |
| E/A | 1.61 | 1.63 | 1.26 |
| Lateral e’ (cm/s) | 14 | 12 | 9 + |
| Septal e’ (cm/s) | 11 | 9 * | 7 |
| Average E/e’ | 8.22 | 9.68 | 11.0 |
| LAVI (mL/m2) | 44.6 | 40.7 | 36.8 |
| Valvular disease | Moderate MR—1 Mitral prolapse—1 (mild) | Mild MR—2 Mild TR—3 | Moderate MR—3 Moderate PR—1 Mild MR—1 Mild TR—2 Mild AS—1 |
| ECG paramaters | |||
| Corrected PQ Interval (ms) | 138 | 146 | 160 |
| Corrected PWD (ms) | 85 | 87 | 98 |
| QRS duration (ms) | 90 | 88 | 102 |
| R wave amplitude lead I (mV) | 5.1 | 6.0 | 10.3+ |
| Sokolow–Lyon index (mV) | 20.7 | 22.6 | 28.0 |
| Sokolow–Lyon LVH criteria | 0/8 | 1/9 (11%) | 13/25 (52%) + |
| Modified Cornell index (mV) | 2.3 | 2.4 | 5.7 |
| Modified Cornell LVH criteria | 0/8 | 0/9 | 4/25 (16%) |
| RBBB criteria | 0/8 | 1/9 (11%) | 4/25 (16%) |
Intervals and voltage are expressed as Means. Categorical variables are expressed as frequency and percentage of occurrence in each group. PWD = P-wave duration; LVH = Left Ventricular Hypertrophy; RBBB = Right bundle branch block; GLS = Global longitudinal strain; LS = longitudinal strain; LVEF = Left ventricular ejection fraction; LAVI = Left atrial volume index; MR = mitral regurgitation; PR = pulmonary regurgitation; TR—tricuspid regurgitation; AS = aortic stenosis, FD = Fabry disease, ECG = electrocardiograph. * p < 0.05 for Group A vs. Group B. + p < 0.05 for Group B vs. Group C.
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. |
© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Share and Cite
MDPI and ACS Style
Zada, M.; Lo, Q.; Trivedi, S.J.; Harapoz, M.; Boyd, A.C.; Devine, K.; Sadick, N.; Tchan, M.C.; Thomas, L. Electrocardiographic Characteristics and Their Correlation with Echocardiographic Alterations in Fabry Disease. J. Cardiovasc. Dev. Dis. 2022, 9, 11. https://doi.org/10.3390/jcdd9010011
AMA Style
Zada M, Lo Q, Trivedi SJ, Harapoz M, Boyd AC, Devine K, Sadick N, Tchan MC, Thomas L. Electrocardiographic Characteristics and Their Correlation with Echocardiographic Alterations in Fabry Disease. Journal of Cardiovascular Development and Disease. 2022; 9(1):11. https://doi.org/10.3390/jcdd9010011
Chicago/Turabian StyleZada, Matthew, Queenie Lo, Siddharth J. Trivedi, Mehmet Harapoz, Anita C. Boyd, Kerry Devine, Norman Sadick, Michel C. Tchan, and Liza Thomas. 2022. "Electrocardiographic Characteristics and Their Correlation with Echocardiographic Alterations in Fabry Disease" Journal of Cardiovascular Development and Disease 9, no. 1: 11. https://doi.org/10.3390/jcdd9010011
Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.
