Serological, Molecular Prevalence and Genotyping of Coxiella burnetii in Dairy Cattle Herds in Northeastern Algeria
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Area
2.2. Sampling
2.3. Laboratory Analysis
2.3.1. Serological Analysis
2.3.2. Molecular Analysis
2.4. Statistical Analysis
3. Results
3.1. Serological Analysis
3.2. Molecular Analysis
3.3. Sequencing
3.4. Statistical Analysis
3.5. Genotyping
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Test | ELISA | Total | ||
---|---|---|---|---|
Positive | negative | 18 | ||
PCR | positive | 10 | 8 | |
negative total | 64 74 | 118 126 | 182 200 | |
Cohen’s kappa: 0.0849 (95% CI: 0.0–0.189) | ||||
Mc Nemar test: 9.06−11 |
Identification Code | Farm Code | Type of Sample | Cycle Threshold Value | Intergenic Spacer | MST Genotype | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cox 2 | Cox 5 | Cox 18 | Cox 20 | Cox 22 | Cox 37 | Cox 51 | Cox 56 | Cox 57 | Cox 61 | |||||
1 | Farm 1 | Whole blood | 31.6 | NA | NA | NA | NA | 5 | NA | NA | 12 | 3 | NA | NI |
2 | Farm 1 | Whole blood | 31.5 | 3 | 5 | 1 | NA | 5 | NA | NA | 12 | 3 | NA | MST32 |
3 | Farm 2 | Whole blood | 30.8 | 3 | 5 | 5 | NA | 5 | NA | NA | NA | NA | 5 | New |
4 | Farm 2 | Whole blood | 30.2 | 3 | 5 | 5 | NA | 5 | 1 | NA | NA | 6 | 5 | New |
5 | Farm 3 | Whole blood | 32.3 | NA | 5 | 1 | NA | 5 | 4 | NA | NA | 3 | NA | MST32 |
6 | Farm 3 | Whole blood | 32.2 | NA | 5 | 1 | NA | 5 | 4 | NA | 12 | 3 | NA | MST32 |
7 | Farm 4 | Whole blood | 26 | 3 | 5 | 1 | 6 | 5 | 4 | 5 | 12 | 3 | 2 | MST32 |
8 | Farm 5 | BMT | 32.7 | 3 | NA | NA | NA | 5 | 4 | NA | NA | NA | NA | NI |
9 | Farm 6 | BMT | 34.9 | 3 | NA | NA | NA | 5 | 4 | NA | NA | NA | NA | NI |
10 | Farm 7 | BMT | 28.2 | 3 | 5 | 1 | 6 | 5 | 4 | 5 | 4 | 3 | 2 | MST12 |
11 | Farm 8 | BMT | 28.3 | 3 | 5 | 1 | 6 | 5 | 4 | 5 | 4 | 3 | 2 | MST12 |
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Menadi, S.E.; Chisu, V.; Santucciu, C.; Di Domenico, M.; Curini, V.; Masala, G. Serological, Molecular Prevalence and Genotyping of Coxiella burnetii in Dairy Cattle Herds in Northeastern Algeria. Vet. Sci. 2022, 9, 40. https://doi.org/10.3390/vetsci9020040
Menadi SE, Chisu V, Santucciu C, Di Domenico M, Curini V, Masala G. Serological, Molecular Prevalence and Genotyping of Coxiella burnetii in Dairy Cattle Herds in Northeastern Algeria. Veterinary Sciences. 2022; 9(2):40. https://doi.org/10.3390/vetsci9020040
Chicago/Turabian StyleMenadi, Salah Eddine, Valentina Chisu, Cinzia Santucciu, Marco Di Domenico, Valentina Curini, and Giovanna Masala. 2022. "Serological, Molecular Prevalence and Genotyping of Coxiella burnetii in Dairy Cattle Herds in Northeastern Algeria" Veterinary Sciences 9, no. 2: 40. https://doi.org/10.3390/vetsci9020040