Transcriptome RNA Sequencing Reveals That Circular RNAs Are Abundantly Expressed in Embryonic Breast Muscle of Duck
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Samples for Circular RNA Sequencing
2.2. Library Construction and Sequencing
2.3. Annotation of Duck circRNAs and Differential Expression Analysis between E13 and E19
2.4. Enrichment Analysis of the Differentially Expressed circRNAs
2.5. cDNA Synthesis and qRT-PCR
2.6. Validation of circRNAs
2.7. Overexpression Plasmid Construction and siRNA Synthesis
2.8. Cell Isolation, Culture, and Electroporation
2.9. Flow Cytometry of Cell Cycle Analysis
2.10. Cell-Counting Assay
3. Results
3.1. Overview of RNA Sequencing Data and Identification of Duck Circular RNA
3.2. Identification of Differentially Expressed Circular RNAs
3.3. Enrichment Analysis of the Differentially Expressed Circular RNAs
3.4. Verification of the Differential Expression Circular RNAs
3.5. Experimental Validation and Spatiotemporal Expression of circGAS2-2
3.6. circGAS2-2 Promotes the Proliferation of Duck Breast Primary Myoblasts
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Liu, J.; Liu, S.; Zhang, W.; Hu, X.; Mao, H.; Liu, S.; Chen, B. Transcriptome RNA Sequencing Reveals That Circular RNAs Are Abundantly Expressed in Embryonic Breast Muscle of Duck. Vet. Sci. 2023, 10, 75. https://doi.org/10.3390/vetsci10020075
Liu J, Liu S, Zhang W, Hu X, Mao H, Liu S, Chen B. Transcriptome RNA Sequencing Reveals That Circular RNAs Are Abundantly Expressed in Embryonic Breast Muscle of Duck. Veterinary Sciences. 2023; 10(2):75. https://doi.org/10.3390/vetsci10020075
Chicago/Turabian StyleLiu, Jing, Shuibing Liu, Wentao Zhang, Xiaolong Hu, Huirong Mao, Sanfeng Liu, and Biao Chen. 2023. "Transcriptome RNA Sequencing Reveals That Circular RNAs Are Abundantly Expressed in Embryonic Breast Muscle of Duck" Veterinary Sciences 10, no. 2: 75. https://doi.org/10.3390/vetsci10020075