Substantially Improved Electrofusion Efficiency of Hybridoma Cells: Based on the Combination of Nanosecond and Microsecond Pulses
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethics Statement and Cell Culture
2.2. Cell Electrofusion Platform
2.3. Fluorescence Experiments
2.4. Hybridoma Culture Experiments
2.5. Definition of Cell Fusion Efficiency
2.6. Electrofusion Protocols
2.7. Statistical Analysis
3. Results
3.1. Fluorescence Experiments
3.2. Culture Experiments
4. Discussion
4.1. Characteristics of Cell Fusion Based on μsPEF (μsHVPEF or μsLVPEF)
4.2. Characteristics of Cell Fusion Based on nsPEF
4.3. Cell Fusion Based on ns/μsLVPEF
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Group | Pulse Type | ns Electric Field (kV/cm) | ns Pulse Width (µs) | ns Number | μs Electric Field (kV/cm) | μs Pulse Width (µs) | μs Number | Total Dose (kV2μs/cm2) |
---|---|---|---|---|---|---|---|---|
1 | ns/μsLVPEF | 6 | 0.2 | 5 | 2 | 40 | 1 | 36 + 160 |
μsHVPEF | 0 | 0 | 0 | 2.2 | 40 | 1 | 196 | |
Control | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
2 | ns/μsLVPEF | 6 | 0.2 | 5 | 2.5 | 40 | 1 | 36 + 250 |
μsHVPEF | 0 | 0 | 0 | 2.67 | 40 | 1 | 286 | |
Control | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
3 | ns/μsLVPEF | 6 | 0.2 | 5 | 3 | 40 | 1 | 36 + 360 |
μsHVPEF | 0 | 0 | 0 | 3.15 | 40 | 1 | 396 | |
Control | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
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Wu, M.; Ke, Q.; Bi, J.; Li, X.; Huang, S.; Liu, Z.; Ge, L. Substantially Improved Electrofusion Efficiency of Hybridoma Cells: Based on the Combination of Nanosecond and Microsecond Pulses. Bioengineering 2022, 9, 450. https://doi.org/10.3390/bioengineering9090450
Wu M, Ke Q, Bi J, Li X, Huang S, Liu Z, Ge L. Substantially Improved Electrofusion Efficiency of Hybridoma Cells: Based on the Combination of Nanosecond and Microsecond Pulses. Bioengineering. 2022; 9(9):450. https://doi.org/10.3390/bioengineering9090450
Chicago/Turabian StyleWu, Meng, Qiang Ke, Jinhao Bi, Xinhao Li, Shuheng Huang, Zuohua Liu, and Liangpeng Ge. 2022. "Substantially Improved Electrofusion Efficiency of Hybridoma Cells: Based on the Combination of Nanosecond and Microsecond Pulses" Bioengineering 9, no. 9: 450. https://doi.org/10.3390/bioengineering9090450