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Article

Modelling Mixed Microbial Culture Polyhydroxyalkanoate Accumulation Bioprocess towards Novel Methods for Polymer Production Using Dilute Volatile Fatty Acid Rich Feedstocks

1
Promiko AB, Briggatan 16, 23442 Lomma, Sweden
2
School of Chemical Engineering, The University of Queensland, Brisbane, QLD 4072, Australia
3
Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy
4
Department of Environmental Sciences, Informatics and Statistics, Cà Foscari University of Venice, Via Torino 155, 30172 Venice, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Giorgos Markou
Bioengineering 2022, 9(3), 125; https://doi.org/10.3390/bioengineering9030125
Received: 9 February 2022 / Revised: 8 March 2022 / Accepted: 16 March 2022 / Published: 21 March 2022
(This article belongs to the Special Issue Advances in Polyhydroxyalkanoate (PHA) Production, Volume 3)
Volatile fatty acid (VFA) rich streams from fermentation of organic residuals and wastewater are suitable feedstocks for mixed microbial culture (MMC) Polyhydroxyalkanoate (PHA) production. However, many such streams have low total VFA concentration (1–10 gCOD/L). PHA accumulation requires a flow-through bioprocess if the VFAs are not concentrated. A flow through bioprocess must balance goals of productivity (highest possible influent flow rates) with goals of substrate utilization efficiency (lowest possible effluent VFA concentration). Towards these goals, dynamics of upshift and downshift respiration kinetics for laboratory and pilot scale MMCs were evaluated. Monod kinetics described a hysteresis between the upshift and downshift responses. Substrate concentrations necessary to stimulate a given substrate uptake rate were significantly higher than the concentrations necessary to sustain the attained substrate uptake rate. A benefit of this hysteresis was explored in Monte Carlo based PHA accumulation bioprocess numerical simulations. Simulations illustrated for a potential to establish continuous flow-through PHA production bioprocesses even at a low (1 gCOD/L) influent total VFA concentration. Process biomass recirculation into an engineered higher substrate concentration mixing zone, due to the constant influent substrate flow, enabled to drive the process to maximal possible PHA production rates without sacrificing substrate utilization efficiency. View Full-Text
Keywords: polyhydroxyalkanoates (PHA); polyhydroxybutyrate (PHB); mixed microbial cultures; activated sludge; respiration kinetics; Monod kinetics; oxygen mass balance; hysteresis; process modelling polyhydroxyalkanoates (PHA); polyhydroxybutyrate (PHB); mixed microbial cultures; activated sludge; respiration kinetics; Monod kinetics; oxygen mass balance; hysteresis; process modelling
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MDPI and ACS Style

Werker, A.; Lorini, L.; Villano, M.; Valentino, F.; Majone, M. Modelling Mixed Microbial Culture Polyhydroxyalkanoate Accumulation Bioprocess towards Novel Methods for Polymer Production Using Dilute Volatile Fatty Acid Rich Feedstocks. Bioengineering 2022, 9, 125. https://doi.org/10.3390/bioengineering9030125

AMA Style

Werker A, Lorini L, Villano M, Valentino F, Majone M. Modelling Mixed Microbial Culture Polyhydroxyalkanoate Accumulation Bioprocess towards Novel Methods for Polymer Production Using Dilute Volatile Fatty Acid Rich Feedstocks. Bioengineering. 2022; 9(3):125. https://doi.org/10.3390/bioengineering9030125

Chicago/Turabian Style

Werker, Alan, Laura Lorini, Marianna Villano, Francesco Valentino, and Mauro Majone. 2022. "Modelling Mixed Microbial Culture Polyhydroxyalkanoate Accumulation Bioprocess towards Novel Methods for Polymer Production Using Dilute Volatile Fatty Acid Rich Feedstocks" Bioengineering 9, no. 3: 125. https://doi.org/10.3390/bioengineering9030125

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