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Open AccessArticle

Gelatin-Methacryloyl (GelMA) Formulated with Human Platelet Lysate Supports Mesenchymal Stem Cell Proliferation and Differentiation and Enhances the Hydrogel’s Mechanical Properties

1
Institute of Technical Chemistry, Gottfried Wilhelm Leibniz Universität Hannover, 30167 Hannover, Germany
2
German Red Cross Blood Service NSTOB, 31832 Springe, Germany
*
Author to whom correspondence should be addressed.
Bioengineering 2019, 6(3), 76; https://doi.org/10.3390/bioengineering6030076
Received: 26 July 2019 / Revised: 19 August 2019 / Accepted: 24 August 2019 / Published: 28 August 2019
(This article belongs to the Special Issue Mesenchymal Stem Cells in Tissue Regeneration)
Three-dimensional (3D) cell culture is a major focus of current research, since cultivation under physiological conditions provides more reliable information about in vivo cell behavior. 3D cell cultures are used in basic research to better understand intercellular and cell-matrix interactions. Moreover, 3D cell culture plays an increasingly important role in the in vitro testing of bioactive substances and tissue engineering. Gelatin-methacryloyl (GelMA) hydrogels of different degrees of functionalization (DoFs) are a versatile tool for 3D cell culture and related applications such as bioprinting. Human platelet lysate (hPL) has already demonstrated positive effects on 2D cell cultures of different cell types and has proven a valuable alternative to fetal calf serum (FCS). Traditionally, all hydrogels are formulated using buffers. In this study, we supplemented GelMA hydrogels of different DoF with hPL during adipose tissue-derived mesenchymal stem cell (AD-MSCs) encapsulation. We studied the effect of hPL supplementation on the spreading, proliferation, and osteogenic differentiation of AD-MSCs. In addition, the influence of hPL on hydrogel properties was also investigated. We demonstrate that the addition of hPL enhanced AD-MSC spreading, proliferation, and osteogenic differentiation in a concentration-dependent manner. Moreover, the addition of hPL also increased GelMA viscosity and stiffness. View Full-Text
Keywords: gelatin-methacryloyl (GelMA); hydrogels; 3D cell culture; human platelet lysate; adipose tissue-derived mesenchymal stem cells (AD-MSCs) gelatin-methacryloyl (GelMA); hydrogels; 3D cell culture; human platelet lysate; adipose tissue-derived mesenchymal stem cells (AD-MSCs)
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MDPI and ACS Style

Kirsch, M.; Birnstein, L.; Pepelanova, I.; Handke, W.; Rach, J.; Seltsam, A.; Scheper, T.; Lavrentieva, A. Gelatin-Methacryloyl (GelMA) Formulated with Human Platelet Lysate Supports Mesenchymal Stem Cell Proliferation and Differentiation and Enhances the Hydrogel’s Mechanical Properties. Bioengineering 2019, 6, 76.

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