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WeReview: CRISPR Tools—Live Repository of Computational Tools for Assisting CRISPR/Cas Experiments

National Centre for Biotechnology (CNB-CSIC). c/ Darwin 3, 28049 Madrid, Spain
Rare Diseases Networking Biomedical Research Centre (CIBERER-ISCIII), 28049 Madrid, Spain
Authors to whom correspondence should be addressed.
Bioengineering 2019, 6(3), 63;
Received: 9 July 2019 / Revised: 17 July 2019 / Accepted: 24 July 2019 / Published: 25 July 2019
PDF [962 KB, uploaded 25 July 2019]


Computational tools are essential in the process of designing a CRISPR/Cas experiment for the targeted modification of an organism’s genome. Among other functionalities, these tools facilitate the design of a guide-RNA (gRNA) for a given nuclease that maximizes its binding to the intended genomic site, while avoiding binding to undesired sites with similar sequences in the genome of the organism of interest (off-targets). Due to the popularity of this methodology and the rapid pace at which it evolves and changes, new computational tools show up constantly. This rapid turnover, together with the intrinsic high death-rate of bioinformatics tools, mean that many of the published tools become unavailable at some point. Consequently, the traditional ways to inform the community about the landscape of available tools, i.e., reviews in the scientific literature, are not adequate for this fast-moving field. To overcome these limitations, we have developed “WeReview: CRISPR Tools,” a live, on-line, user-updatable repository of computational tools to assist researchers in designing CRISPR/Cas experiments. In its web site users can find an updated comprehensive list of tools and search for those fulfilling their specific needs, as well as proposing modifications to the data associated with the tools or the incorporation of new ones.
Keywords: CRISPR/Cas; genome editing; computational tool CRISPR/Cas; genome editing; computational tool
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Torres-Perez, R.; Garcia-Martin, J.A.; Montoliu, L.; Oliveros, J.C.; Pazos, F. WeReview: CRISPR Tools—Live Repository of Computational Tools for Assisting CRISPR/Cas Experiments. Bioengineering 2019, 6, 63.

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