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Bioengineering of a Full-Thickness Skin Equivalent in a 96-Well Insert Format for Substance Permeation Studies and Organ-On-A-Chip Applications

1
TissUse GmbH, D-13347 Berlin, Germany
2
Institute of Biotechnology, Department Medical Biotechnology, Technische Universität Berlin, D-13355 Berlin, Germany
3
Institute of Bioprocess and Biosystem Engineering, Hamburg University of Technology, D-21073 Hamburg, Germany
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Bioengineering 2018, 5(2), 43; https://doi.org/10.3390/bioengineering5020043
Received: 4 May 2018 / Revised: 30 May 2018 / Accepted: 1 June 2018 / Published: 7 June 2018
(This article belongs to the Special Issue Advanced Dynamic Cell and Tissue Culture)
The human skin is involved in protecting the inner body from constant exposure to outer environmental stimuli. There is an evident need to screen for toxicity and the efficacy of drugs and cosmetics applied to the skin. To date, animal studies are still the standard method for substance testing, although they are currently controversially discussed Therefore, the multi-organ chip is an attractive alternative to replace animal testing. The two-organ chip is designed to hold 96-well cell culture inserts (CCIs). Small-sized skin equivalents are needed for this. In this study, full-thickness skin equivalents (ftSEs) were generated successfully inside 96-well CCIs. These skin equivalents developed with in vivo-like histological architecture, with normal differentiation marker expressions and proliferation rates. The 96-well CCI-based ftSEs were successfully integrated into the two-organ chip. The permeation of fluorescein sodium salt through the ftSEs was monitored during the culture. The results show a decreasing value for the permeation over time, which seems a promising method to track the development of the ftSEs. Additionally, the permeation was implemented in a computational fluid dynamics simulation, as a tool to predict results in long-term experiments. The advantage of these ftSEs is the reduced need for cells and substances, which makes them more suitable for high throughput assays. View Full-Text
Keywords: full thickness skin equivalents; multi-organ chip; substance permeation; 96-well cell culture insert full thickness skin equivalents; multi-organ chip; substance permeation; 96-well cell culture insert
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MDPI and ACS Style

Schimek, K.; Hsu, H.-H.; Boehme, M.; Kornet, J.J.; Marx, U.; Lauster, R.; Pörtner, R.; Lindner, G. Bioengineering of a Full-Thickness Skin Equivalent in a 96-Well Insert Format for Substance Permeation Studies and Organ-On-A-Chip Applications. Bioengineering 2018, 5, 43.

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