Next Article in Journal
Exploration of Cytokines That Impact the Therapeutic Efficacy of Mesenchymal Stem Cells in Alzheimer’s Disease
Previous Article in Journal
Channel-Dependent Multilayer EEG Time-Frequency Representations Combined with Transfer Learning-Based Deep CNN Framework for Few-Channel MI EEG Classification
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Bioengineering
Volume 12
Issue 6
10.3390/bioengineering12060644
Review Report
bioengineering-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
Brief Report
Peer-Review Record

Large-Scale Expansion of Suspension Cells in an Automated Hollow-Fiber Perfusion Bioreactor

Bioengineering 2025, 12(6), 644; https://doi.org/10.3390/bioengineering12060644
by Eric Bräuchle 1,2,*, Maria Knaub 3, Laura Weigand 1,2, Elisabeth Ehrend 1, Patricia Manns 1, Antje Kremer 3, Hugo Fabre 3 and Halvard Bonig 1,4,5
Reviewer 1: Anonymous
Reviewer 2:
Mei-Jin Guo
Reviewer 3:
Pornthap Thanonkeo
Bioengineering 2025, 12(6), 644; https://doi.org/10.3390/bioengineering12060644
Submission received: 24 April 2025 / Revised: 28 May 2025 / Accepted: 6 June 2025 / Published: 12 June 2025
(This article belongs to the Section Cellular and Molecular Bioengineering)

Round 1

Reviewer 1 Report

Comments and Suggestions for Authors

I am delighted to review the manuscript entitled „Large-scale expansion of suspension cells in an automated hollow-fiber perfusion bioreactor“.

Congrats to the authors for the very well-written manuscript. The text is well-structured, with a strong focus and scientific impact on bioengineering in cell therapy.

I have just a few minor questions:

-Please provide more informations for the here applied hardware (provide a technical depiction, scheme etc.).

-Do the authors have experience with human cell lines in terms of the here described experimental set-up? Please comment!

Author Response

Please see the attachment.

Author Response File: Author Response.docx

Reviewer 2 Report

Comments and Suggestions for Authors

please see the attached file.

Comments for author File: Comments.pdf

Author Response

Please see the attachment.

Author Response File: Author Response.docx

Reviewer 3 Report

Comments and Suggestions for Authors

This research article effectively describes the cultivation of suspension cells using an automated hollow-fiber perfusion bioreactor. It is commendable that the experiments are well-designed, and the results align with the experimental design, providing interesting information. While most of the manuscript's content is sound, a few points could be further improved to enhance its overall completeness and impact. These are:

  1. Abstract: It is recommended that the abstract be merged into a single paragraph.
  2. Materials and Methods: To enhance the credibility and clarity of the methodology, the number of experimental replications for each experiment should be clearly stated. Specifying the number of replications is crucial for assessing the precision and reproducibility of the results.
  3. Conclusion: The conclusion would be strengthened and offer greater value if it were adjusted to explicitly highlight the key findings of this study. Furthermore, it should clearly indicate how these findings can be applied to future research or practical applications.

Author Response

Please see the attachment.

Author Response File: Author Response.docx

Back to TopTop