Human-Derived Cortical Neurospheroids Coupled to Passive, High-Density and 3D MEAs: A Valid Platform for Functional Tests
Abstract
:1. Introduction
2. Material and Methods
2.1. Human-Induced Pluripotent Stem Cells Generation and Maintenance
2.2. Neuronal Differentiation and Neurospheroids Generation
2.3. MEAs Devices
2.4. Data Acquisition and Analysis
2.5. Electrical Stimulation and Neuromodulation
2.6. Immunofluorescence
2.7. Statistical Analysis
3. Results
3.1. Human iPSCs-Derived Neurons Aggregate into Neurospheroids
3.2. Neurospheroids Showed Electrophysiological Activity on MEAs
3.3. Neuromodulation Affects Spontaneous Activity and Electrical Induced Activity
3.4. Neuromodulation Affects Connectivity and Signal Propagation
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Muzzi, L.; Di Lisa, D.; Falappa, M.; Pepe, S.; Maccione, A.; Pastorino, L.; Martinoia, S.; Frega, M. Human-Derived Cortical Neurospheroids Coupled to Passive, High-Density and 3D MEAs: A Valid Platform for Functional Tests. Bioengineering 2023, 10, 449. https://doi.org/10.3390/bioengineering10040449
Muzzi L, Di Lisa D, Falappa M, Pepe S, Maccione A, Pastorino L, Martinoia S, Frega M. Human-Derived Cortical Neurospheroids Coupled to Passive, High-Density and 3D MEAs: A Valid Platform for Functional Tests. Bioengineering. 2023; 10(4):449. https://doi.org/10.3390/bioengineering10040449
Chicago/Turabian StyleMuzzi, Lorenzo, Donatella Di Lisa, Matteo Falappa, Sara Pepe, Alessandro Maccione, Laura Pastorino, Sergio Martinoia, and Monica Frega. 2023. "Human-Derived Cortical Neurospheroids Coupled to Passive, High-Density and 3D MEAs: A Valid Platform for Functional Tests" Bioengineering 10, no. 4: 449. https://doi.org/10.3390/bioengineering10040449