Identification of Antimycobacterial Natural Products from a Library of Marine Invertebrate Extracts
Abstract
:1. Introduction
2. Results and Discussion
2.1. Screening of Marine Invertebrate Samples
2.2. Dereplication, Molecular Networking, Isolation and Structure Elucidation
2.3. Antimycobacterial Activity of Isolated Compounds and Semi-Pure Fractions
3. Materials and Methods
3.1. General Experimental Procedure
3.2. Marine Invertebrate Samples
3.3. Antimycobacterial Activity
3.4. Fractionation, Isolation and Purification of Active Compounds
3.5. Dereplication and Molecular Networking
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Country | Phylum | Class | Order | Family | Genus | Species | NPID | MIC90 (μg/mL) | MIC99 (μg/mL) |
---|---|---|---|---|---|---|---|---|---|
Mauritius | Porifera | Demospongiae | Astrophorida | Ancorinidae | Jaspis | splendens (Dorypleres) | C019765 | 2.4 | 4.5 |
Porifera | Demospongiae | Dictyoceratida | Thorectidae | Hyrtios | reticulatus | C019725 | 51.0 | 72.0 | |
South Africa | Porifera | Demospongiae | Poecilosclerida | Guitarridae | Guitarra | fimbriata indica | C018520 | 70.0 | 87.0 |
Porifera | Demospongiae | Hadromerida | Suberitidae | Aaptos | sp. 1 | C018442 | 71.0 | 83.0 | |
Porifera | Demospongiae | Verongida | Aplysinellidae | Porphyria | pedunculata | C018530 | 71.0 | 94.0 | |
Porifera | Demospongiae | Poecilosclerida | Hymedesmiidae | Phorbas | sp. 1 | C018462 | 72.0 | 81.0 | |
Porifera | Demospongiae | Halichondrida | Heteroxyidae | Higginsia | bidentifera | C018466 | 72.0 | 83.0 | |
Porifera | Demospongiae | Halichondrida | Heteroxyidae | Higginsia | sp. 1 | C018578 | 72.0 | 84.0 | |
Cnidaria | Hydrozoa | Leptothecatae | Aglaopheniidae | Lytocarpia | formosa | C018502 | 73.0 | 81.0 | |
Porifera | Demospongiae | Halichondrida | Halichondriidae | Hymeniacidon | sp. 1 | C018470 | 73.0 | 84.0 | |
Porifera | Demospongiae | Poecilosclerida | Guitarridae | Guitarra | fimbriata indica | C018458 | 73.0 | 91.0 | |
Chordata | Ascidiacea | Aplousobranchia | Didemnidae | Didemnum | obscurum | C019906 | 1.6 | 1.7 | |
Porifera | Demospongiae | Halichondrida | Dictyonellidae | Stylissa | sp. 1, n.sp. | C019904 | 17.0 | 19.4 | |
Cnidaria | Anthozoa | Alcyonacea | Nephtheidae | Drifa | sp. b (n.sp.) | C018631 | 41.0 | 52.0 | |
Porifera | Demospongiae | Poecilosclerida | Raspailiidae | Echinodictyum | sp. 1 | C018566 | 25.1 | 42.8 | |
Mollusca | Polyplacophora | Chitonida | Acanthochitonidae | Acanthochitona | garnoti | C018637 | 0.5 | 0.63 | |
Porifera | Demospongiae | Poecilosclerida | Isodictyidae | Isodictya | sp. 2, n.sp. | C018626 | 18.8 | 6.25 | |
Porifera | Demospongiae | Poecilosclerida | Tedaniidae | Hemitedania | sp. 1 | C018538 | 18.7 | 9.3 | |
Porifera | Demospongiae | Halichondrida | Halichondriidae | Halichondria | sp. 1 | C018460 | 13.8 | 12.2 | |
Tanzania | Porifera | Demospongiae | Haplosclerida | Petrosiidae | Neopetrosia | tuberosa cf. | C015405 | 2.5 | 3.2 |
Porifera | Demospongiae | Haplosclerida | Phloeodictyidae | Oceanapia | ramsayi | C015331 | 22.0 | 28.0 | |
Porifera | Demospongiae | Haplosclerida | Phloeodictyidae | Oceanapia | sp. 3 | C015461 | 35.0 | 42.0 | |
Porifera | Demospongiae | Poecilosclerida | Chondropsidae | Chondropsis | sp. 1 | C015479 | 58.0 | 64.0 | |
Chordata | Ascidiacea | Aplousobranchia | Polycitoridae | Eudistoma | giganteum | C015357 | 61.0 | 68.0 | |
Porifera | Demospongiae | Dictyoceratida | Thorectidae | Aplysinopsis | elegans cf. | C015228 | 2.5 | 2.60 | |
Mollusca | Gastropoda | Neogastropoda | Cypraeidae | Cypraea | tigris | C015272 | 47.9 | 57.9 | |
Porifera | Demospongiae | Poecilosclerida | Podospongiidae | Diacarnus | ardoukobae | C015180 | 70.8 | 81.2 | |
Papua-New Guinea | Chordata | Ascidiacea | Aplousobranchia | Didemnidae | Lissoclinum | badium | C018795 | 2.6 | 3.1 |
Porifera | Demospongiae | Dictyoceratida | Thorectidae | Fascaplysinopsis | sp. 2 | C018781 | 7.9 | 16.5 | |
Porifera | Demospongiae | Haplosclerida | Petrosiidae | Petrosia (Strongylophora) | corticata | C018743 | 31.0 | 35.0 | |
Porifera | Demospongiae | Dictyoceratida | Dysideidae | Dysidea | sp. 17 | C018675 | 32.0 | 36.0 | |
Porifera | Demospongiae | Haplosclerida | Chalinidae | Haliclona (Gellius) | sp. 4 | C018717 | 36.0 | 57.0 | |
Porifera | Demospongiae | Dictyoceratida | Dysideidae | Lamellodysidea | herbacea | C018667 | 41.0 | 43.0 | |
Porifera | Demospongiae | Dictyoceratida | Thorectidae | Carteriospongia | sp. 3 | C018787 | 48.0 | 70.0 | |
Porifera | Demospongiae | Dictyoceratida | Thorectidae | Phyllospongia | sp. 7 | C018595 | 63.0 | 70.0 | |
Porifera | Demospongiae | Dictyoceratida | Thorectidae | Aplysinopsis | elegans | C018651 | 65.0 | 71.0 | |
Porifera | Demospongiae | Poecilosclerida | Mycalidae | Mycale | setosa | C018783 | 72.0 | 81.0 | |
Porifera | Demospongiae | Dictyoceratida | Spongiidae | Spongia | sp. 5 | C018671 | 72.0 | 82.0 | |
Chordata | Ascidiacea | Aplousobranchia | Polyclinidae | Synoicum | castellatum | C018601 | 72.0 | 83.0 | |
Porifera | Demospongiae | Haplosclerida | Petrosiidae | Neopetrosia | tuberosa cf. | C018593 | 72.0 | 83.0 | |
Porifera | Demospongiae | ‘Lithistid’ | Theonellidae | Theonella | sp. 7 | C018729 | 73.0 | 83.0 | |
Porifera | Demospongiae | Dictyoceratida | Irciniidae | Ircinia | arbuscula cf. | C018773 | < 0.24 | 0.5 | |
Porifera | Demospongiae | Hadromerida | Suberitidae | Aaptos | nigra | C018695 | 58.3 | 51.4 | |
Porifera | Demospongiae | Agelasida | Agelasidae | Agelas | oxeata cf. | C018756 | 0.4 | 0.9 | |
Porifera | Demospongiae | ‘Lithistid’ | Scleritodermidae | Aciculites | oxtylota | C018740 | 20.8 | 21.9 | |
Porifera | Demospongiae | ‘Lithistid’ | Scleritodermidae | Microscleroderma | herdmani | C018684 | 23.7 | 30.6 | |
Porifera | Demospongiae | Haplosclerida | Callyspongiidae | Callyspongia (Euplacella) | elongata cf. | C018714 | 85.2 | 125.0 | |
Porifera | Demospongiae | Haplosclerida | Niphatidae | Pachychalina | sp. 11 | C018694 | 17.9 | 46.5 | |
Porifera | Demospongiae | Astrophorida | Thrombidae | Thrombus | sp. 1 | C018716 | 20.8 | 42.5 | |
Porifera | Demospongiae | Haplosclerida | Niphatidae | Niphates | elegans | C018720 | 23.8 | 91.3 | |
Palau Islands | Porifera | Demospongiae | Halichondrida | Halichondriidae | Topsentia | cavernosa | C019960 | 71.9 | 78.9 |
Porifera | Demospongiae | Hadromerida | Suberitidae | Aaptos | nigra | C019928 | 85.2 | 106.0 | |
Porifera | Demospongiae | Haplosclerida | Chalinidae | Haliclona (Gellius) | sp. 4 | C020034 | 95.9 | 120.0 | |
Porifera | Demospongiae | Verongida | Aplysinellidae | Porphyria | sp. 1, n.sp. | C019898 | 113.0 | 125.0 | |
Control | Rifampicin | 0.02 | 0.03 |
Compound/Fraction | MIC90 (µg/mL): 7 Days | MIC90 (µg/mL): 14 Days |
---|---|---|
2 | 2.14 | 1.03 |
3 | 62.50 | 31.25 |
F107 | 1.09 | 0.74 |
F114 | <0.24 | <0.24 |
F130 | 0.65 | 0.49 |
Rifampicin | 0.02 | 0.01 |
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Acquah, K.S.; Beukes, D.R.; Seldon, R.; Jordaan, A.; Sunassee, S.N.; Warner, D.F.; Gammon, D.W. Identification of Antimycobacterial Natural Products from a Library of Marine Invertebrate Extracts. Medicines 2022, 9, 9. https://doi.org/10.3390/medicines9020009
Acquah KS, Beukes DR, Seldon R, Jordaan A, Sunassee SN, Warner DF, Gammon DW. Identification of Antimycobacterial Natural Products from a Library of Marine Invertebrate Extracts. Medicines. 2022; 9(2):9. https://doi.org/10.3390/medicines9020009
Chicago/Turabian StyleAcquah, Kojo Sekyi, Denzil R. Beukes, Ronnett Seldon, Audrey Jordaan, Suthananda N. Sunassee, Digby F. Warner, and David W. Gammon. 2022. "Identification of Antimycobacterial Natural Products from a Library of Marine Invertebrate Extracts" Medicines 9, no. 2: 9. https://doi.org/10.3390/medicines9020009