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Article

Inhibition of Angiogenic Factor Productions by Quercetin In Vitro and In Vivo

1
Department of Physiology, Showa University School of Medicine, Tokyo 142-8555, Japan
2
Department of Medical Education, Showa University School of Medicine, Tokyo 142-8555, Japan
3
Faculty of Human Sciences, University of Human Arts and Sciences, Saitama 339-8555, Japan
*
Author to whom correspondence should be addressed.
Medicines 2021, 8(5), 22; https://doi.org/10.3390/medicines8050022
Received: 3 February 2021 / Revised: 6 May 2021 / Accepted: 8 May 2021 / Published: 12 May 2021
(This article belongs to the Special Issue Feature Papers in Allergic Diseases)
Background: Angiogenesis is well known to be an important event in the tissue remodeling observed in allergic diseases. Although there is much evidence that quercetin, one of the most abundant dietary flavonoids, exerts anti-allergic effects in both human and experimental animal models of allergic diseases, the action of quercetin on angiogenesis has not been defined. Therefore, in this study, we first examined the action of quercetin on the secretion of angiogenic factors from murine mast cells in vitro. We also examined the action of quercetin on angiogenic factor secretion in the murine allergic rhinitis model in vivo. Methods: Mast cells (1 × 105 cells/mL) sensitized with ovalbumin (OVA)-specific murine IgE were stimulated with 10.0 ng/mL OVA in the presence or the absence of quercetin for 24 h. The concentrations of angiogenic factors, vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), tumor necrosis factor-α, IL-6 and IL-8 in the supernatants were examined by ELISA. BALB/c male mice immunized with OVA were challenged intranasally with OVA every other day, starting seven days after the final immunization. These mice were then orally administered quercetin once a day for five days, starting seven days after the final immunization. Clinical symptoms were assessed by counting the number of sneezes and nasal rubbing behaviors during the 10 min period just after OVA nasal provocation. The angiogenic factor concentrations in the nasal lavage fluids obtained 6 h after nasal antigenic provocation were examined by ELISA. Results: Quercetin significantly inhibited the production of angiogenetic factors induced by IgE-dependent mechanisms at 5.0 µM or more. Oral administration of 25.0 mg/kg quercetin into the mice also suppressed the appearance of angiogenetic factors in nasal lavage fluids, along with the attenuation of nasal symptoms. Conclusions: These results strongly suggest that the inhibitory action of quercetin on angiogenic factor secretion may be implicated in the therapeutic action of quercetin on allergic diseases, especially allergic rhinitis. View Full-Text
Keywords: quercetin; allergic rhinitis; angiogenic factor; mast cell; suppression; in vitro; in vivo quercetin; allergic rhinitis; angiogenic factor; mast cell; suppression; in vitro; in vivo
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MDPI and ACS Style

Okumo, T.; Furuta, A.; Kimura, T.; Yusa, K.; Asano, K.; Sunagawa, M. Inhibition of Angiogenic Factor Productions by Quercetin In Vitro and In Vivo. Medicines 2021, 8, 22. https://doi.org/10.3390/medicines8050022

AMA Style

Okumo T, Furuta A, Kimura T, Yusa K, Asano K, Sunagawa M. Inhibition of Angiogenic Factor Productions by Quercetin In Vitro and In Vivo. Medicines. 2021; 8(5):22. https://doi.org/10.3390/medicines8050022

Chicago/Turabian Style

Okumo, Takayuki, Atsuko Furuta, Tarou Kimura, Kanako Yusa, Kazuhito Asano, and Masataka Sunagawa. 2021. "Inhibition of Angiogenic Factor Productions by Quercetin In Vitro and In Vivo" Medicines 8, no. 5: 22. https://doi.org/10.3390/medicines8050022

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