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Synergistic Effects of Danshen (Salvia Miltiorrhizae Radix et Rhizoma) and Sanqi (Notoginseng Radix et Rhizoma) Combination in Angiogenesis Behavior in EAhy 926 Cells

National Institute of Complementary Medicine (NICM), Western Sydney University, Penrith, NSW 2751, Australia
South Western Sydney Clinical School, UNSW Medicine, University of New South Wales, Kensington 2052, Australia
School of Biological Sciences, Nanyang Technological University, Singapore 637551, Singapore
School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool L3 3AF, UK
Faculty of Sciences, TCM Division, University of Technology Sydney, Ultimo, NSW 2007, Australia
Authors to whom correspondence should be addressed.
Academic Editor: William N. Setzer
Medicines 2017, 4(4), 85;
Received: 16 October 2017 / Revised: 15 November 2017 / Accepted: 15 November 2017 / Published: 21 November 2017
(This article belongs to the Special Issue Bioactivities and Medical Use of Herbs and Plants)
PDF [4815 KB, uploaded 21 November 2017]


Background: This study investigated the combination effects of the Danshen and Sanqi herb pair on angiogenesis in vitro. Methods: Nine combination ratios of Danshen-Sanqi extracts (DS-SQ) were screened for their angiogenic effects in the human vascular endothelial EAhy 926 cell line via cell proliferation, cell migration and tube formation activities against the damage to the cells exerted by DL-homocysteine (Hcy) and adenosine (Ado). The type of interaction (synergistic, antagonistic, additive) between Danshen and Sanqi was analyzed using combination index (CI) and isobologram models. The angiogenic activities of key bioactive compounds from Danshen and Sanqi were tested in the same models. Results: DS-SQ ratios of 2:8 and 3:7 (50–300 µg/mL) potentiated angiogenic synergistic effects (CI < 1) in all three assays. The observed wound healing effects of DS-SQ 2:8 was significantly attenuated by phosphatidylinositol-3 kinases (PI3K), mitogen-activated protein kinase (MEK) and extracellular signal-regulated kinases (ERK) inhibitors which inferred the potential mechanistic pathways. Out of all the tested compounds, Notoginsenoside R1 from Sanqi exhibited the most potent bioactivity in cell proliferation assay. Conclusions: This study provides scientific evidence to support the traditional use of the Danshen-Sanqi combination for vascular disease, in particular through their synergistic interactions on previously unexamined angiogenic pathways. View Full-Text
Keywords: Danshen-Sanqi; synergy; angiogenesis Danshen-Sanqi; synergy; angiogenesis

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Zhou, X.; Razmovski-Naumovski, V.; Kam, A.; Chang, D.; Li, C.; Bensoussan, A.; Chan, K. Synergistic Effects of Danshen (Salvia Miltiorrhizae Radix et Rhizoma) and Sanqi (Notoginseng Radix et Rhizoma) Combination in Angiogenesis Behavior in EAhy 926 Cells. Medicines 2017, 4, 85.

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