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ShenLingLan Influences the Attachment and Migration of Ovarian Cancer Cells Potentially through the GSK3 Pathway

Cardiff China Medical Research Collaborative, Cardiff University-Peking University Joint Cancer Institute, Henry Wellcome Building, School of Medicine, Cardiff University, Cardiff CF14 4XN, UK
Department of Gynaecological Oncology, Peking University Cancer Hospital, Haidian District, Beijing 100142, China
Departments of Gynaecology and Medical Oncology, YuHuangDing Hospital, QingDao University, Yantai 266061, China
Yiling Medical Research Institute, Shijiazhuang 050000, China
State Key Laboratory of Collateral Disease Research and Innovation Medicine, Shijiazhuang 050000, China
Key Disciplines of State Administration of TCM for Collateral Disease, Shijiazhuang 050000, China
Authors to whom correspondence should be addressed.
Academic Editors: Sivarama Vinjamury and Elizabeth Sommers
Medicines 2017, 4(1), 10;
Received: 18 November 2016 / Revised: 25 January 2017 / Accepted: 15 February 2017 / Published: 21 February 2017
(This article belongs to the Special Issue Complementary and Alternative Medicine for Cancer Patients)
PDF [2742 KB, uploaded 21 February 2017]


Background: Ovarian cancer presents a major clinical challenge in the UK. Glycogen synthase kinase-3 (GSK-3) has been linked to cancer. This study tested the impact of ShenLingLan (SLDM) on ovarian cancer cell behaviour and its links to GSK-3. Methods: Fresh ovarian tumours (n = 52) were collected and processed. Histopathologcial and clinical information were collected and analysed against GSK-3 transcript levels using quantitative PCR (qPCR). Immortalised ovarian cancer cells’ protein alterations in response to SLDM were identified using a Kinexus™ protein kinase array. The effects of SLDM and a combination of SLDM and TWS119 on ovarian cancer cells ability to attach and migrate were evaluated using electrical cell-substrate impedance sensing (ECIS). Results: Transcript expression of GSK-3β was significantly increased in ovarian tumours which were poorly differentiated, patients with recurrence and in patients who had died from ovarian cancer. Treating SKOV-3 ovarian cells with SLDM reduced GSK-3 expression and GSK-3α (Y279). Treatment with SLDM reduced ovarian cancer cells ability to attach and migrate, which was further reduced in the presence of TWS119. Conclusions: This study identified a potential mechanism by which SLDM may exert anti-metastatic effects. Further work is needed to investigate the in vivo effects SLDM has on ovarian tumours. View Full-Text
Keywords: ShenLingLan; ovarian cancer; cell migration; GSK3 ShenLingLan; ovarian cancer; cell migration; GSK3

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Owen, S.; Ruge, F.; Gao, Y.; Yang, Y.; Hou, J.; Chen, J.; Feng, Y.; Zhang, H.; Gao, Y.; Wang, H.; Wei, C.; Wu, Y.; Jiang, W.G. ShenLingLan Influences the Attachment and Migration of Ovarian Cancer Cells Potentially through the GSK3 Pathway. Medicines 2017, 4, 10.

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