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Article

Influence of Different Isolation Methods on Chemical Composition and Bioactivities of the Fruit Peel Oil of Citrus medica L. var. sarcodactylis (Noot.) Swingle

1
Department of Biotechnology, Zhejiang Normal University, Jinhua 321004, China
2
Department of Chemistry, University of Alabama in Huntsville, Huntsville, AL 35899, USA
*
Author to whom correspondence should be addressed.
Medicines 2017, 4(1), 1; https://doi.org/10.3390/medicines4010001
Submission received: 18 November 2016 / Revised: 22 December 2016 / Accepted: 27 December 2016 / Published: 4 January 2017

Abstract

:
Background: The chemical composition and bioactivities of essential oils (EOs) of fingered citron (Citrus medica L. var. sarcodactylis (Noot.) Swingle) are considerably sensitive and lapsible during high-temperature processing of traditional separating techniques. In the present research, vacuum distillation and ultrafiltration were utilized in order to process the concentrated juice from fingered citron, obtaining a high-quality essential oil. Methods: In order to compare the essential oils obtained by conventional means, the chemical compositions of the essential oils were analyzed using GC-MS, before antimicrobial and antioxidant screening assays were carried out. Results: Oil which had been subjected to vacuum distillation was shown to maintain most of the distinctiveness of the fingered citron, due to its high content of characteristic flavor components and low content of cyclic oxygenated monoterpenoids. Interestingly, the oil obtained by ultrafiltration showed notable in vitro antimicrobial activity. The DPPH· radical-scavenging assay method revealed that the antioxidant abilities were as follows, presented in descending order: vacuum distillation oil > hydrodistillation oil > ultrafiltration oil. Conclusions: The essential oil obtained by vacuum distillation could be combined with the juice produced from fingered citron to create one of the most promising techniques in the fine-processing of citron fruits.

Graphical Abstract

1. Introduction

Citrus medica L. var. sarcodactylis (Noot.) Swingle, commonly known as fingered citron in China, is an uncommon variation of Citrus medica L., belonging to the Rutaceae family [1,2]. It is well-known as a precious Chinese traditional medicine and is widely cultivated from the central area of China, to the southeastern coast, especially in the Jinhua district of the Zhejiang province, lying to the south of Shanghai [3]. The fruits are widely manufactured for use in various types of processed foods, such as healthcare tea, drink flavoring, succade, and traditional desserts. Also, the intact fresh fruits could be directly sold or used as decorative bonsai at international markets, extending from Asia to South America, mostly due to their unique finger-like appearance and exquisite aroma. However, recently, due to the requirement for the modernization of traditional Chinese herbs, attention has been focused on research relating to bioactive components and the development of advanced processing techniques for fingered citron. In particular, as both a medicinal and edible product, it has been acknowledged that the essential oil of fingered citron is a major component of the commercial sector, with an extensive use as a flavoring agent for beverages, ice cream [4], biscuits, cakes, healthcare food [5], and household products [6,7]. Therefore, in terms of commercialization of fingered citron, it is of utmost importance that we obtain a high-quality essential oil, at the lowest cost possible, during the processing of the fruits.
Unlike other citrus fruits such as lemon and grapefruit, mature fingered citron has only a thin yellow exocarp and thick white entoderm, without juicy pulp or seeds [2]. This peculiar anatomical structure, results in a low proportion of exocarp containing essential oil. Therefore, the total yield of a complete fruit is typically in the range of 0.7%–1.2% (w/w), as stated in previously published work [8,9]. Furthermore, the oil-water state is sometimes impossible to separate, depending on single-circle hydrodistillation, due to the emulsification of a much lower essential oil content into a relatively large amount of water. Therefore, a conventional isolation process for producing essential oil from these fruits usually employs hydrodistillation using condensed fluid, repeatedly returning to the evaporator-like Clevenger apparatus. However, for a practical large-scale production of essential oil, the cost of isolating the essential oil needs to be considered, and the essential oil could be combined with other procedures, such as beverage production. Much research has focused on oil separation following the cold-pressing of citrus fruits, with pulp such as the common orange [10], but few investigations have pursued using fingered citrons, which have no pulp.
The quality of essential oils produced from fingered citron is highly dependent on its chemical composition, as well as associated bioactivities [11]. During the course of processing the peels of mature fruits, different isolation techniques can not only result in different oil yields, but can also have noticeable effects on chemical composition and other related oil qualities [12,13]. Therefore, owing to potential commercial applications, there have been several investigations that have attempted to demonstrate the composition and stability of characteristic aromas, and to determine possible bioactivities, such as those which are antimicrobial or antioxidant [14,15]. The GC-MS analysis of oils from Japanese fingered citron showed that β-ionone, one of the aroma components with a very low odor threshold (7 ng/L), was responsible for the characteristic osmanthus-like aroma of the fruit [16]. However, more recent studies have determined the characteristic aromatic components of essential oils from the Jinhua district of China, the results of which, using gas chromatography-olfactometry (GC-O), have demonstrated that α-pinene and α-bergamotene were the principal contributors to the characteristic aroma of fingered citron oil [17]. In addition, as a traditional Chinese medicinal plant, fingered citron has usually been employed as a stomachic carminative, asthma expectorant, and anxiolytic [1,9]. Although an increasing number of oil bioactivities, including hypotensive and hypolipidemic effects, have been confirmed [5], there have been no reports on the effects of isolation procedures on the chemical composition and bioactivities.
This work presents two innovative isolation techniques for directly separating the essential oils from the juices of fingered citron. It is important to note that both of the methodologies proposed here do not represent conventional solid-water mixing distillation of essential oils, but a new separation method of the concentrated upper layer of fruit juice. One is a short-time vacuum distillation, and the other is ultrafiltration. The data reported in this work were obtained by comparing the chemical compositions and bioactivities of fingered citron oils using the new methods, with those of the oils obtained by conventional methods such as hydrodistillation and organic solvent extraction. The purpose of this research is to obtain a high-quality, commercially applicable fingered citron oil, that can maintain the pure distinctiveness of fingered citron. It is very likely that improving the quality of fingered citron oil, as well as reducing the processing cost, would significantly improve local agricultural production.

2. Materials and Methods

2.1. Materials

The fresh fruits of Citrus medica L. var. sarcodactylis Swingle were harvested in November 2014, from Jinhua (29.11° N, 119.64° E, 55 m above sea level), Zhejiang, China. The essential oil isolation procedures of fingered citron were performed as presented in Figure 1. The vacuum distillation was maintained at 65–85 °C, according to the distillate flow rate. The ultrafitrating operation was completed at room temperature, and the filtration rate was only 17.5–21 mL/h. The yields of vacuum distillation oil, ultrafiltration oil, and hydrodistillation oil, were 0.73% ± 0.12%, 0.28% ± 0.09%, and 1.07% ± 0.14%, respectively.

2.2. Gas Chromatographic/Mass Spectral Analysis

The essential oils of fingered citron were analyzed by GC-MS [18], using an Agilent 6890 GC (Agilent Technologies, Palo Alto, CA, USA) with an Agilent 5973 mass selective detector (Agilent Technologies, Palo Alto, CA, USA, operated in the EI mode (electron energy = 70 eV), scan range = 40–400 mAU, and scan rate = 3.99 scans/s), and an Agilent ChemStation data system (G1701CA, Agilent Technologies, Palo Alto, CA, USA). The GC column was a HP-5ms fused silica capillary with a (5% phenyl)-polymethylsiloxane stationary phase, a film thickness of 0.25 μm, a length of 30 m, and an internal diameter of 0.25 mm. The carrier gas was helium, with a column head pressure of 48.7 kPa and a flow rate of 1.0 mL/min. Injector temperature was 200 °C and interface temperature was 280 °C. The GC oven temperature program was used as follows: 40 °C initial temperature, held for 10 min; increased at 3 °C/min to 200 °C; increased 2 °C/min to 220 °C. A 1% w/v solution of the sample in CH2Cl2 was prepared and 1 μL was injected using a splitless injection technique.
Identification of the oil components was based on their retention indices, determined by the reference to a homologous series of n-alkanes, and by a comparison of their mass spectral fragmentation patterns with those reported in the literature [19] and stored on the MS library (NIST database (Version G1036A, Revision D.01.00, US Department of Commerce, Gaithersburg, MD, USA)/ChemStation data system (Version G1701CA, Revision C.00.01.080, Agilent Technologies, Palo Alto, CA, USA)). The percentages of each component are reported as raw percentages, based on total ion current without standardization.

2.3. Antimicrobial Screening

The essential oils were screened for antibacterial activity against: Gram-positive bacteria, Staphylococcus aureus (ATCC No. 29213), Bacillus cereus (ATCC No. 14579); Gram-negative bacteria Pseudomonas aeruginosa (ATCC No. 27853), Serratia marcescens (ATCC No. 14756). Minimum inhibitory concentrations (MIC) were determined by using the microbroth dilution technique [20]. Dilutions of the essential oil were prepared in cation-adjusted Mueller Hinton broth (CAMHB), beginning with 50 μL of 1% w/w solutions of essential oil in DMSO, plus 50 μL CAMHB. The extract solutions were serially diluted (1:1) in CAMHB, in 96-well plates. Organisms at a concentration of approximately 1.5 × 108 colony forming units (CFU)/mL were added to each well. Plates were incubated at 37 °C for 24 h; the final minimum inhibitory concentration (MIC) was determined as the lowest concentration without turbidity. Gentamycin was used as a positive antibiotic control; DMSO was used as a negative control. Antifungal activity against Candida albicans (ATCC No. 10231) and Aspergillus niger (ATCC No. 16888) was determined as above, using Yeast and Mold Broth (YMB) inoculated with the corresponding fungal culture, diluted to a McFarland turbidity of 1.0. Amphotericin B was the positive control [18].

2.4. Antioxidant Activity Screening

The samples were also subjected to screening in order to identify their possible antioxidant activity, by using a 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH·) scavenging assay [21]. Free radical scavenging activity of the corresponding samples was dependent on the extent of bleaching of the purple-colored DPPH methanol solution. Aliquots (50 μL) of various concentrations (0.5–3.0 mg/mL) of the samples in methanol were added to 5 mL of 0.004% DPPH solution in methanol (5 mL). Following a 30-min incubation period at room temperature, the absorbance was recorded against a blank at 517 nm. Inhibition of DPPH· in percentage (I%) was calculated using the following formula:
I D P P H % = ( 1 A 0 A x ) × 100
where A0 is the absorbance of the control reaction (containing all reagents except the test compound), and Ax is the absorbance of the test sample. The median inhibitory concentration (IC50) was calculated from the plot of inhibition percentage, against sample concentration. Tests were carried out in triplicate.

3. Results

3.1. Essential Oil Compositions

The essential oils obtained by the different isolation procedures are characterized by the presence of cyclic and acyclic monoterpenes, which may or may not possess oxygen atoms in their structures (Table 1). All of oil components are divided into eight groups, including cyclic monoterpene hydrocarbons (I), acyclic monoterpene hydrocarbons (II), acyclic oxygenated monoterpenoids (III), aldehydes (IV), cyclic oxygenated monoterpenoids (V), sesquiterpene hydrocarbons (VI), oxygenated sesquiterpenoids (VII), and coumarins (VIII).

3.2. Antimicrobial Activity of Fingered Citron Oils

The MICs of three fingered citron oils against Gram-positive bacteria, Gram-negative bacteria, and fungi, are reported in Table 2. In particular, the ultrafiltration oil showed notable in vitro antimicrobial activity against Staphylococcus aureus (MIC = 156 μg/mL), Bacillus cereus (MIC = 156 μg/mL), Pseudomonas aeruginosa (MIC = 39 μg/mL), and Aspergillus niger (MIC = 20 μg/mL).The vacuum distillation oil was only marginally active against Staphylococcus aureus (MIC = 625 μg/mL), Bacillus cereus (MIC = 313 μg/mL), and Pseudomonas aeruginosa (MIC = 625 μg/mL), but was also strongly active against Aspergillus niger (MIC = 156 μg/mL).

3.3. Antioxidant Activity

The antioxidant activity of essential oils is another biological property of great interest for potential utilization in cosmetic supplements or functional food products. The use of a DPPH· scavenging assay is the most routinely practiced method for the assessment of anti-radical properties of different samples. As shown in Figure 2, the antioxidant efficiency of the tested essential oils primarily relies on their concentration, and their free radical scavenging effectiveness was as follows, given in descending order and based on the increasing median inhibitory concentration (IC50) values: vacuum distillation oil (1.02 mg/mL) > hydrodistillation oil (1.19 mg/mL) > ultrafiltration oil (1.42 mg/mL).

4. Discussions

The cyclic monoterpenes (I) not containing oxygen atoms are common components in the essential oils of most citrus fruit peels. Previous work has suggested that the mechanism for the formation of p-cymene occurs during a rearrangement reaction in which limonene rearranges to form terpinenes, which then dehydrogenates to form p-cymene, while pinene and limonene can interconvert with each other through isomerization (Figure 3) [22]. Oils obtained using three different methods have no significant differences in their pinene (α- or β-) content. However, when only focusing on the three most abundant compounds (limonene, γ-terpinene, and p-cymene), the oil obtained by vacuum distillation possessed a relatively low content of limonene (47.6%), a relatively high content of γ-terpinene (29.5%), and an absence of p-cymene, while the oil obtained by hydrodistillation revealed limonene (60.1%), γ-terpinene (6.1%), and p-cymene (17.6%). Notably, the oil produced from ultrafiltration had the highest limonene (64.7%) and p-cymene content (26.1%), and the lowest γ-terpinene content (0.1%). The differences in content between these three compounds are most likely related to the considerable interconversion which occurs during the isolation process (Figure 1). The significantly different isolation conditions, including pressure, temperature, and time, seems to have largely influenced the rates of interconversion. For example, ultrafiltration is a slow procedure with an outflow speed of only 20–30 mL/h, so it was necessary to complete this at relatively high pressures (0.15–0.18 MPa) for long periods of time (10–15 h), while vacuum distillation is almost complete within 0.5–1 h, at a pressure of 0.03–0.05 MPa and a temperature of 80–85 °C.
Generally, the diagnostic index for good quality citrus oils largely depends on the quantities of two types of compounds; acyclic monoterpene hydrocarbons (II) and acyclic oxygenated monoterpenoids (III) [23]. By comparing the relative ratio (RR) in Table 3, of either quantity of compounds or their contents, the value of oil from vacuum distillation is of a much higher quality than the oils produced from the other two procedures. Conversely, the concentration of oxygenated terpenoids, including cyclic oxygenated monoterpenoids (V) and oxygenated sesquiterpenoids (VII), can indicate the likelihood that oils have been exposed to the air or light for long periods during isolation or storage. These two classes of compounds can sometimes result in a few unpleasant odors, which will have negative consequences for the oil quality. As shown in Table 3, the relative ratios of cyclic oxygenated monoterpenoids are 8.7 (ultrafiltration), 2.3 (hydrodistillation), and 3.8 (organic solvent extraction) [16], respectively. Low concentrations of the cyclic oxygenated monoterpenoids and the absence of oxygenated sesquiterpenoids, are also characteristic of the oil isolated by vacuum distillation. Relatively quick operation and vacuum conditions minimize the time that the oils are exposed to air and considerably high temperatures, thus achieving high quality oils.
It is of utmost importance that the oils obtained retain the distinctive aroma compounds of the fingered citron fruit. β-Ionone, one of the oxygenated sesquiterpenoids, was thought to be the most characteristic aroma component in the peel of fingered citron [16], but it was not detected in the oils from Zhejiang, China, using three different isolation methods. More recently, gas chromatography-olfactometry (GC-O) has been used to analyze the main odorous compounds in fingered citron essential oil [17]. The results demonstrated that α-pinene and α-bergamotene were the key compounds contributing to the characteristic aroma of fingered citron oil. In this research, the content of α-bergamotene in vacuum distilled oil was greater than that in oils obtained from ultrafiltration or hydrodistillation, while the concentrations of α-pinene in the three types of oils were very similar. These results can help to explain the intensity of the aroma of the oil produced using vacuum distillation.
When analysing conventional hydrodistillation oil, either antibacterial activity or antifungal activity was extremely weak. In previous work, the oils from conventional hydrodistillation had shown weak antibacterial activity in zone-of-inhibition assays, that was partly consistent with the present results, but antifungal inhibitory concentrations were not determined [24]. The antimicrobial activity of the ultrafiltration oil can be attributed to the high concentration of p-cymene. This compound has shown antibacterial activity against Bacillus cereus [25] and Escherichia coli [26], as well as antifungal activity against several Candida spp. [27] and filamentous fungi [28].
The different antioxidant activities can be mainly ascribed to their complex compositions, which have different chemical profiles. Since an essential oil is not a pure single compound, its activities often refer to the actions of inter-components, such as synergism and antagonism, which are rarely experimentally supported, thus remaining speculative. However, they can be used to explain the observed bioactivities [14]. Isolation methods are generally believed to result in a variation of naturally occurring antioxidants in the raw materials of a plant. Intense thermal treatment or prolonged air-exposure may be responsible for the oxygenated reactions of monoterpenes or sesquiterpenes in fingered citron oils. Extensive formation of oxygenated products might have significant negative effects on their free radical scavenging effectiveness. More specifically, even when ultrafiltration is carried out at room temperature (20–25 °C), the resulting oils showed lower free radical-scavenging activity, in addition to a marked loss of monoterpene or sesquiterpene hydrocarbons, and an evident increase of oxygenated terpenoids.

5. Conclusions

In this work, the influence of different isolation methods on the chemical composition and bioactivities of the fruit peel oil from fingered citron were studied in detail. The various isolation methods included significantly different pressures, temperatures, and duration times, which have the potential to affect the reaction rates of interconversion, such as rearrangement, isomerization, and dehydrogenation. Compared to the oils obtained from other isolation methods, the vacuum distillation oil possessed a comparatively high ability to maintain more characteristic components and fewer oxygenated products (cyclic oxygenated monoterpenoids). In addition, the bioactivity screening assays showed that the ultrafiltration oil displayed notable in vitro antimicrobial activity against some bacteria and mold, while vacuum distillation oil had a relatively high antioxidant activity. In sum, the vacuum distillation method, combined with the juice production of high-quality fingered citron oil, could be thought of as one of the most promising techniques for the fine-processing of citron fruits.

Acknowledgments

This work was financially supported by Science and Technology Key Project (No. 2013C02032) and Public Technical Project (No. 2012C22084) of Zhejiang province in China. We thank Professor Joyce Kelly for her assistance with GC-MS measurements.

Author Contributions

The list authors contributed to this work as follows: Gang Deng and William N. Setzer conceived and designed the experiments; Jonathan D. Craft, Kelly Marie Steinberg and Suraj Kumar Pokharel performed antimicrobial screening assay and GC-MS spectral analysis; Pei Lei Li performed antimicrobial activity assay. All authors read and approved the final manuscript.

Conflicts of Interest

The authors declare no conflict of interest.

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Figure 1. Scheme showing different isolation methods of essential oil from fingered citron fruits. a The average diameter of fruit peel particle is around 2–3 mm; b The absolute pressure in the vacuum is 0.03–0.05 MPa (absolute pressure); c The pore diameter of microfiltration is 0.45 μm; d The molecular weight cut-off (MWCO) of ultrafiltration is 4000 Da.
Figure 1. Scheme showing different isolation methods of essential oil from fingered citron fruits. a The average diameter of fruit peel particle is around 2–3 mm; b The absolute pressure in the vacuum is 0.03–0.05 MPa (absolute pressure); c The pore diameter of microfiltration is 0.45 μm; d The molecular weight cut-off (MWCO) of ultrafiltration is 4000 Da.
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Figure 2. DPPH· scavenging effectiveness of tree oils and ascorbic acid (Vc). Data are expressed as means ± SD of three experiments.
Figure 2. DPPH· scavenging effectiveness of tree oils and ascorbic acid (Vc). Data are expressed as means ± SD of three experiments.
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Figure 3. Potential conversion of pinene to p-cymene.
Figure 3. Potential conversion of pinene to p-cymene.
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Table 1. Peak Identification and Weight Percentage for the Components of Essential Oils.
Table 1. Peak Identification and Weight Percentage for the Components of Essential Oils.
EntryCompoundsKI aVD Oil cUF Oil dHD Oil eRef Oil f
RI bArea (%)RIArea (%)RIArea (%)RIArea (%)
(I) cyclic monoterpene hydrocarbons
1α-thujene9309310.6499320.1929330.5529241.34
2α-pinene9399363.3179382.4409383.7219342.92
3camphene9549440.03
4sabinene9759740.1439740.0659670.40
5β-pinene9799761.8879781.7939761.9529752.70
6α-phellandrene100210030.2649990.09
7α-terpinene101710160.68810120.68
8p-cymene1024102326.099102617.56110150.13
9limonene1029103547.599102964.667103160.129102247.79
10γ-terpinene1059106429.45510580.05010586.172104932.08
11terpinolene108810891.65110870.15410841.37
12p-cymenene109110900.072
(II) acyclic monoterpene hydrocarbons
13β-myrcene9909921.5519910.6539911.7869821.67
14(Z)-β-ocimene103710405.91410385.09810310.36
15(E)-β-ocimene105010501.37710480.60110390.52
16allo-ocimene113211300.345
(III) acyclic oxygenated monoterpenoids
17linalool109611010.11310870.15
18citronellal115311530.05511320.17
19nerol122912270.123
20(Z)-ocimenone122912280.273
21neral123812400.70612160.38
22geraniol125212530.09312191.56
23linalyl acetate125712100.10
24geranial126712690.94512380.45
25neryl acetate136113640.01813640.02213640.05913410.04
26geranyl acetate138113840.01813850.02913850.10313590.05
(IV) aldehydes
27nonanal115010830.02
28decanal120112000.01411870.00
(V) cyclic oxygenated monoterpenoids
29cis-limonene oxide113611190.02
30trans-limonene oxide1142
31terpinen-4-ol117711730.12111770.18511760.07111660.13
32p-cymen-8-ol118211850.26611840.077
33α-terpineol118811850.09111900.07611900.05511770.67
34trans-carveol121612170.28412170.129
35cis-carveol122912280.099
36carvone124312400.78612410.063
37carvyl acetate128212790.234
(VI) sesquiterpene hydrocarbons
38δ-elemene133813350.026
39α-cubebene134813470.014
40β-cubebene138813880.007
41β-elemene139013900.010
42α-cis-bergamotene141214130.02714140.01614130.020
43β-caryophyllene141914160.48814160.01214250.13
44α-trans-bergamotene143414340.45314340.30014330.38714360.13
45γ-elemene143614980.08
46α-humulene145414510.041
47β-trans-farnesene145614570.04414550.030
48germacrene D148514790.51914800.01214810.01714840.28
49β-ionone148814680.03
50bicyclogermacrene150014950.108
51α-bisabolene150315030.04415010.020
52β-bisabolene150515070.51015030.42915070.55615030.21
52δ-cadinene152315230.016
(VII) oxygenated sesquiterpenoids
54caryophyllene oxide158315850.13215850.260
(VIII) coumarins
555,7-dimethoxycoumarin193719340.27
Total99.44299.01199.74996.951
a Kovats index (KI) on DB-5 in reference to n-alkanes [19]; b Experimental retention index on a HP-5 ms column; c Oils obtained from vacuum distillation of the top layer of cold-pressed juice; d Oils obtained from ultrafiltration of the top layer of cold-pressed juice; e Oils obtained from conventional hydrodistillation of fruit peels; f Oils extracted by using organic solvent (n-pentane and dichloromethane, 1/1 wt/wt) by Shiota [16].
Table 2. Antimicrobial activity of fingered citron peel oils.
Table 2. Antimicrobial activity of fingered citron peel oils.
SampleAntimicrobial Activity (MIC, μg/mL)
S. aureus (G+) aB. cereus (G+)P. aeruginosa (G−) bS. marcescens (G−)C. albicans (Fungus)A. niger (Fungus)
Vacuum distillation oil62531362512502500156
Ultrafiltration oil15615639625125020
Hydrodistillation oil12501250125012502500625
a G+ is short for Gram-positive; b G− is short for Gram-negative.
Table 3. Summary Analysis of the Classes of Components in the Essential Oils.
Table 3. Summary Analysis of the Classes of Components in the Essential Oils.
No.CompoundsVD OilUF OilHD OilRef Oil
Count aSTC b (%)RR cCountSTC (%)RRCountSTC (%)RRCountSTC (%)RR
(I)cyclic monoterpene hydrocarbons985.6541795.3131.113890.3051.0541189.531.045
(II)acyclic monoterpene hydrocarbons49.187110.6530.07137.4850.81532.550.278
(III)acyclic oxygenated monoterpenoids82.071130.3240.15620.1620.07882.901.400
(IV)aldehydes10.014100000020.021.492
(V)cyclic oxygenated monoterpenoids20.211161.8328.68260.4932.33530.823.886
(VI)Sesquiterpene hydrocarbons142.306140.7570.32871.0440.45360.860.373
(VII)Oxygenated sesquiterpenoids00-10.132-10.260-00-
(VIII)coumarins00-00-00-10.27-
Sum of Identified Compounds38222734
Featuring Aroma Intensityvery intenselightmedium intensenot given
a subtotal number for each type of compound; b subtotal content for each type of compound; c relative ratio (RR) to subtotal content of VD oil.

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Deng, G.; Craft, J.D.; Steinberg, K.M.; Li, P.L.; Pokharel, S.K.; Setzer, W.N. Influence of Different Isolation Methods on Chemical Composition and Bioactivities of the Fruit Peel Oil of Citrus medica L. var. sarcodactylis (Noot.) Swingle. Medicines 2017, 4, 1. https://doi.org/10.3390/medicines4010001

AMA Style

Deng G, Craft JD, Steinberg KM, Li PL, Pokharel SK, Setzer WN. Influence of Different Isolation Methods on Chemical Composition and Bioactivities of the Fruit Peel Oil of Citrus medica L. var. sarcodactylis (Noot.) Swingle. Medicines. 2017; 4(1):1. https://doi.org/10.3390/medicines4010001

Chicago/Turabian Style

Deng, Gang, Jonathan D. Craft, Kelly Marie Steinberg, Pei Lei Li, Suraj Kumar Pokharel, and William N. Setzer. 2017. "Influence of Different Isolation Methods on Chemical Composition and Bioactivities of the Fruit Peel Oil of Citrus medica L. var. sarcodactylis (Noot.) Swingle" Medicines 4, no. 1: 1. https://doi.org/10.3390/medicines4010001

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