Next Article in Journal
Antifungal and Anticancer Potential of Argemone mexicana L.
Next Article in Special Issue
Isolation and Cytotoxic Investigation of Flacourtin from Oncoba spinosa
Previous Article in Journal / Special Issue
Methanol Extract from Anogeissus leiocarpus (DC) Guill. et Perr. (Combretaceae) Stem Bark Quenches the Quorum Sensing of Pseudomonas aeruginosa PAO1
Article Menu

Export Article

Open AccessArticle
Medicines 2016, 3(4), 27;

Chemical Composition, Cytotoxic, Apoptotic and Antioxidant Activities of Main Commercial Essential Oils in Palestine: A Comparative Study

Deptartment of Nutrition and Food Technology, An-Najah National University, PO Box 7, Nablus, 415, Palestine
Department of Food and Nutritional Sciences, The University of Reading, Whiteknights, Reading RG6 6AP, UK
Deptartment of Chemistry, An-Najah National University, PO Box 7, Nablus 415, Palestine
Author to whom correspondence should be addressed.
Academic Editor: James D. Adams
Received: 1 September 2016 / Revised: 16 October 2016 / Accepted: 19 October 2016 / Published: 25 October 2016
(This article belongs to the Special Issue Plant Medicines for Clinical Trial)
Full-Text   |   PDF [1419 KB, uploaded 2 November 2016]   |  


Background: Essential oils (EOs) are complex mixtures of several components gifted with a wide array of biological activities. The present research was designed to evaluate whether commercial essential oils could be effective by examining their in vitro antioxidant, cytotoxic, and apoptotic properties of nine commercially available EOs in Palestine, namely, African rue, basil, chamomile, fennel, fenugreek, ginger, spearmint, sage, and thyme, and to assure their effective use. Methods: The cytotoxic activity was determined using HT29-19(A) non-muco secreting and HT29-muco secreting (MS) cell lines. MTT, and trypan blue tests, and DPPH radical scavenging have also been assayed on the studied EOs. Results: In this work chamomile oil showed the lowest IC50 at the content of 60 µL/mL, while all other EOs reached such a decrease when 70–80 µL/mL was used on HT-29 (MS) cell lines. In HT-29 19(A) cells, 50% of viability was obtained when 80 µL/mL of ginger and African rue was used, while all other EOs needed more than 80 µL/mL to reach such a decline in viability. Otherwise, an MTT assay on HT-29 (MS) displayed ginger EO with the lowest IC50, followed by African rue and sage, with 40, 48 and 53 µL/mL, respectively. Otherwise, for the rest of the EOs, the IC50 was obtained by assaying around 80 µL/mL. Ginger showed the lowest IC50 with 60 µL/mL and thyme was the highest with 77 µL/mL when HT-29 19(A) cells were used. Conclusion: The most active EOs were found to be ginger, chamomile oil, and African rue. In general, the results demonstrate that most commercial EOs tested in this work possess low, or no biological activities; this may be due to processing, storage conditions, and handling or other reasons, which may cause losses in the biological and pharmacological properties that endemically exist in the Eos; hence, more investigation is still required on commercial EOs before they are recommended to the public. View Full-Text
Keywords: essential oils (EOs); cytotoxicity; apoptosis; antioxidants; cell lines; anti-cancer activity essential oils (EOs); cytotoxicity; apoptosis; antioxidants; cell lines; anti-cancer activity

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

A. Al-Tamimi, M.; Rastall, B.; M. Abu-Reidah, I. Chemical Composition, Cytotoxic, Apoptotic and Antioxidant Activities of Main Commercial Essential Oils in Palestine: A Comparative Study. Medicines 2016, 3, 27.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Medicines EISSN 2305-6320 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top