Contact Angle and Cell Adhesion of Micro/Nano-Structured Poly(lactic-co-glycolic acid) Membranes for Dental Regenerative Therapy
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fabrication of the Micro/Nano-Structured PLGA Membrane
2.2. Scanning Electron and Laser Microscopy
2.3. Contact Angle of the Micro/Nano-Structured PLGA Membranes
2.4. Cell Culture
2.5. Cell Adhesion Test
2.6. Confocal Laser Scanning Microscopy
2.7. Statistical Analysis
3. Results
3.1. SEM Images of the Patterned PLGA Membranes
3.2. Laser Microscope Images of the Patterned PLGA Membranes
3.3. Measurement of Contact Angles of the Patterned PLGA Membranes
3.4. Cell Adhesion test on the Patterned PLGA Membranes
3.5. CLSM Images of Actin Filaments
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Pattern Type | Groove | Pillar | Hole | |||||||
---|---|---|---|---|---|---|---|---|---|---|
a | b | c | d | e | f | g | h | i | ||
Pattern size | Width (μm) | 2.0 ± 0.01 | 1.0 ± 0.01 | 0.5 ± 0.02 | 2.0 ± 0.01 | 1.0 ± 0.03 | 0.5 ± 0.04 | 2.0 ± 0.03 | 1.0 ± 0.04 | 0.5 ± 0.05 |
Height (μm) | 1.0 ± 0.01 | 1.0 ± 0.01 | 1.0 ± 0.03 | 1.0 ± 0.02 | 1.0 ± 0.06 | 0.9 ± 0.05 | 1.0 ± 0.07 | 0.9 ± 0.05 | 0.9 ± 0.7 |
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Kaga, N.; Fujimoto, H.; Morita, S.; Yamaguchi, Y.; Matsuura, T. Contact Angle and Cell Adhesion of Micro/Nano-Structured Poly(lactic-co-glycolic acid) Membranes for Dental Regenerative Therapy. Dent. J. 2021, 9, 124. https://doi.org/10.3390/dj9110124
Kaga N, Fujimoto H, Morita S, Yamaguchi Y, Matsuura T. Contact Angle and Cell Adhesion of Micro/Nano-Structured Poly(lactic-co-glycolic acid) Membranes for Dental Regenerative Therapy. Dentistry Journal. 2021; 9(11):124. https://doi.org/10.3390/dj9110124
Chicago/Turabian StyleKaga, Naoyuki, Hiroki Fujimoto, Sho Morita, Yuichiro Yamaguchi, and Takashi Matsuura. 2021. "Contact Angle and Cell Adhesion of Micro/Nano-Structured Poly(lactic-co-glycolic acid) Membranes for Dental Regenerative Therapy" Dentistry Journal 9, no. 11: 124. https://doi.org/10.3390/dj9110124