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Volume 11
Issue 3
10.18683/germs.2021.1274
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Article

Prevalence of Anti-SARS-CoV-2 IgG Antibodies in a Group of Patients, a Control Group, and Healthcare Workers of Thrace Area in Greece, by the Use of Two Distinct Methods

by
Theocharis Konstantinidis
1,2,*,
Stavroula Zisaki
1,
Ioannis Mitroulis
3,
Dimitrios Cassimos
4,
Ioanna Nanousi
1,
Eftychia G. Kontekaki
1,
Vasilis Petrakis
5,
Kalliopi Parrisi
1,
Eleni Fotiadou
2,
Aikaterini Linardou
1,
Nikolaos Lemonakis
2,
Anastasia Grapsa
2,
Theodora Gioka
2,
Leonidas Lazidis
1,
Charalampos Papagoras
3,
Chistina Tsigalou
2,
Periklis Panagopoulos
5,
Panagiotis Skendros
3,
Georges Martinis
1 and
Maria Panopoulou
2
1
Blood Transfusion Center, University General Hospital of Alexandroupolis Dragana Campus, 68100 Alexandroupolis, Greece
2
Laboratory of Microbiology, Democritus University of Thrace, University General Hospital of Alexandroupolis Dragana Campus, 68100 Alexandroupolis, Greece
3
First Department of Internal Medicine, Democritus University of Thrace, Dragana Campus, 68100 Alexandroupolis, Greece
4
Pediatric Department, Democritus University of Thrace, Dragana Campus, 68100 Alexandroupolis, Greece
5
Second Department of Internal Medicine, Democritus University of Thrace, Dragana Campus, 68100 Alexandroupolis, Greece
*
Author to whom correspondence should be addressed.
GERMS 2021, 11(3), 372-380; https://doi.org/10.18683/germs.2021.1274
Submission received: 12 February 2021 / Revised: 11 June 2021 / Accepted: 1 August 2021 / Published: 29 September 2021
Versions Notes

Abstract

Introduction: The aim of this study was to assess the clinical performance of different automated immunoassays available in Europe to detect anti-SARS-CoV-2 antibodies; an ELISA assay and a CLIA. The second goal was to estimate the seroprevalence of SARS-CoV-2 antibodies among healthcare workers in Evros area during the first pandemic wave of COVID-19. Methods: The study included serum samples from 101 patients with confirmed COVID-19 by RT-PCR and 208 negative patients. Furthermore, it included 1036 healthcare workers (HWs) of the Evros Region, Northern Greece. The measurement of anti-SARS-CoV-2 antibodies was performed using the Abbott SARS-CoV-2 IgG and anti-SARS-CoV-2 ELISA IgG assay (Epitope Diagnostics, USA). Results: Of 101 confirmed COVID-19 patients, 82 were hospitalized and 19 were outpatients. Hospitalized patients had higher IgG levels in comparison to outpatients (6.46 ± 2.2 vs. 3.52 ± 1.52, p < 0.001). Of 208 non−COVID-19 patients only 1 was positive in both ELISA and CLIA assay. SARS-CoV-2-IgG antibodies were detected in 6 HWs out of 1036 (0.58%) with mean S/CO-value of anti-SARS-CoV-2 IgG 3.12 ± 1.3 (confidence interval 0.95), which was lower than in COVID-19 patients (3.12 vs. 5.9; p = 0.016). The clinical evaluation of two immunoassays showed remarkably high true positivity rates in the confirmed COVID-19 patients. Sensitivities obtained with CLIA and ELISA methods were 99.02% vs. 97.09% and specificities 99.52% vs 99.05% respectively. Conclusions: We found an acceptable accordance between CLIA and ELISA assays in the confirmed COVID-19 patients. In all subjects included in this study in the past medical history, the information that was obtained included details about the presence of autoimmune diseases.
Keywords: COVID-19; Anti-SARS-CoV-2 Antibodies; SARS-CoV-2; Diagnosis; RT-PCR; Global Health Problem COVID-19; Anti-SARS-CoV-2 Antibodies; SARS-CoV-2; Diagnosis; RT-PCR; Global Health Problem

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MDPI and ACS Style

Konstantinidis, T.; Zisaki, S.; Mitroulis, I.; Cassimos, D.; Nanousi, I.; Kontekaki, E.G.; Petrakis, V.; Parrisi, K.; Fotiadou, E.; Linardou, A.; et al. Prevalence of Anti-SARS-CoV-2 IgG Antibodies in a Group of Patients, a Control Group, and Healthcare Workers of Thrace Area in Greece, by the Use of Two Distinct Methods. GERMS 2021, 11, 372-380. https://doi.org/10.18683/germs.2021.1274

AMA Style

Konstantinidis T, Zisaki S, Mitroulis I, Cassimos D, Nanousi I, Kontekaki EG, Petrakis V, Parrisi K, Fotiadou E, Linardou A, et al. Prevalence of Anti-SARS-CoV-2 IgG Antibodies in a Group of Patients, a Control Group, and Healthcare Workers of Thrace Area in Greece, by the Use of Two Distinct Methods. GERMS. 2021; 11(3):372-380. https://doi.org/10.18683/germs.2021.1274

Chicago/Turabian Style

Konstantinidis, Theocharis, Stavroula Zisaki, Ioannis Mitroulis, Dimitrios Cassimos, Ioanna Nanousi, Eftychia G. Kontekaki, Vasilis Petrakis, Kalliopi Parrisi, Eleni Fotiadou, Aikaterini Linardou, and et al. 2021. "Prevalence of Anti-SARS-CoV-2 IgG Antibodies in a Group of Patients, a Control Group, and Healthcare Workers of Thrace Area in Greece, by the Use of Two Distinct Methods" GERMS 11, no. 3: 372-380. https://doi.org/10.18683/germs.2021.1274

APA Style

Konstantinidis, T., Zisaki, S., Mitroulis, I., Cassimos, D., Nanousi, I., Kontekaki, E. G., Petrakis, V., Parrisi, K., Fotiadou, E., Linardou, A., Lemonakis, N., Grapsa, A., Gioka, T., Lazidis, L., Papagoras, C., Tsigalou, C., Panagopoulos, P., Skendros, P., Martinis, G., & Panopoulou, M. (2021). Prevalence of Anti-SARS-CoV-2 IgG Antibodies in a Group of Patients, a Control Group, and Healthcare Workers of Thrace Area in Greece, by the Use of Two Distinct Methods. GERMS, 11(3), 372-380. https://doi.org/10.18683/germs.2021.1274

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