Exhaustion and Senescence of CD4 and CD8 T Cells That Express Co-Stimulatory Molecules CD27 and CD28 in Subjects That Acquired HIV by Drug Use or by Sexual Route
Abstract
Introduction
Methods
Study population
Real time RT-PCR
Flow cytometry
Statistical analysis
Results
T cell progression to late differentiated stage in HIV groups
T cell senescence in HIV infected patients
Activation of CD4 and CD8 T cells from HIV infected patients
Discussion
Conclusions
Author Contributions
Funding
Conflicts of Interest
Ethics Statement
References
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| Controls | PWID | PWHS | t test | |
|---|---|---|---|---|
| N=18 | N=22 | N=12 | ||
| Age (years), mean ± SD | 27.46±2.75 | 32.45±7.29 | 34.33±10.08 | 0.009 a |
| Gender (female/male) | 13/6 | 1/21 | 3/9 | <0.001 b |
| Drug use, n/N | 0/18 | 22/22 | 0/12 | - |
| HCV infection | 0/18 | 21/22 | 1/18 | <0.001 b |
| HIV viral load (copies/mL), Median [CI] | NA | 108000 [37900–265166] | 30974 [15300–87400] | Ns |
| CD4 (cells/µL), mean ± SD | 769±205 | 443±266 | 324±106 | <0.001 c |
| CD8 (cells/µL), mean ± SD | 436±190 | 1117±553 | 851±522 | <0.001 c |
| CD4/CD8 ratio, mean ± SD | 1.95±0.65 | 0.49±0.29 | 0.49±0.25 | <0.001 c |
| CD4+HLADR+CD38+ (%) | 0.45±0.28 | 7.91±8.76 | 3.13±2.54 | 0.027 c |
| CD8+HLADR+CD38+ (%) | 0.651±0.462 | 20.25±14.78 | 11.26±4.84 | <0.001 c |
| A. | ||
| CD4+HLADR+CD38+ | Control | HIV |
| vs CD4+CD27+CD28+ | r=−0.741; p<0.001 | r=−0.543; p=0.001 |
| vs CD4+CD27+CD28+PD1+ | r=0.871; p<0.001 | r=0.653; p<0.001 |
| vs CD4+CD27+CD28+CD57+ | r=0.845; p<0.001 | r=0.761; p<0.001 |
| vs CD4+CD27-CD28+CD57+ | r=0.435; p=0.018 | r=0.567; p<0.001 |
| vs CD8+CD27+CD28+ | r=−0.593; p=0.009 | r=−0.551; p<0.001 |
| vs CD8+CD27+CD28+PD1+ | r=0.756; p<0.001 | r=0.550; p<0.001 |
| vs CD8+CD27+CD28+CD57+ | r=0.770; p<0.001 | r=0.613; p<0.001 |
| B. | ||
| CD8+HLADR+CD38+ | Control | HIVs |
| vs CD4+CD27+CD28+ | r=−0.496; p=0.036 | ns |
| vs CD4+CD27+CD28+PD1+ | r=0.535; p=0.022 | r=0.604; p<0.001 |
| vs CD4+CD27+CD28+CD57+ | r=0.498; p=0.036 | r=0.552; p<0.001 |
| vs CD4+CD27-CD28+PD1+ | ns | r=0.444; p=0.009 |
| vs CD4+CD27-CD28+CD57+ | ns | r=0.583; p<0.001 |
| vs CD8+CD27+CD28+ | ns | ns |
| vs CD8+CD27+CD28+PD1+ | r=0.492; p=0.038 | r=0.544; p=0.001 |
| vs CD8+CD27+CD28+CD57+ | ns | r=0.428; p=0.013 |
© GERMS 2021.
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Bănică, L.; Vlaicu, O.; Jipa, R.; Abagiu, A.; Nicolae, I.; Neaga, E.; Oţelea, D.; Paraschiv, S. Exhaustion and Senescence of CD4 and CD8 T Cells That Express Co-Stimulatory Molecules CD27 and CD28 in Subjects That Acquired HIV by Drug Use or by Sexual Route. GERMS 2021, 11, 66-77. https://doi.org/10.18683/germs.2021.1242
Bănică L, Vlaicu O, Jipa R, Abagiu A, Nicolae I, Neaga E, Oţelea D, Paraschiv S. Exhaustion and Senescence of CD4 and CD8 T Cells That Express Co-Stimulatory Molecules CD27 and CD28 in Subjects That Acquired HIV by Drug Use or by Sexual Route. GERMS. 2021; 11(1):66-77. https://doi.org/10.18683/germs.2021.1242
Chicago/Turabian StyleBănică, Leontina, Ovidiu Vlaicu, Raluca Jipa, Adrian Abagiu, Ionelia Nicolae, Emil Neaga, Dan Oţelea, and Simona Paraschiv. 2021. "Exhaustion and Senescence of CD4 and CD8 T Cells That Express Co-Stimulatory Molecules CD27 and CD28 in Subjects That Acquired HIV by Drug Use or by Sexual Route" GERMS 11, no. 1: 66-77. https://doi.org/10.18683/germs.2021.1242
APA StyleBănică, L., Vlaicu, O., Jipa, R., Abagiu, A., Nicolae, I., Neaga, E., Oţelea, D., & Paraschiv, S. (2021). Exhaustion and Senescence of CD4 and CD8 T Cells That Express Co-Stimulatory Molecules CD27 and CD28 in Subjects That Acquired HIV by Drug Use or by Sexual Route. GERMS, 11(1), 66-77. https://doi.org/10.18683/germs.2021.1242
