Cell Length Growth in the Fission Yeast Cell Cycle: Is It (Bi)linear or (Bi)exponential?
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains, Media, and Film Techniques
2.2. Cell Length Measurements and Model Fittings
2.3. Further Analyses of Bilinear Patterns
3. Results
3.1. Growth Patterns in Different Cell Cultures
3.2. Further Growth Pattern Analyses of the Adequate Bilinear Cases
3.3. Analysis of Growth Phases in Wild-Type Cells
3.4. Analysis of Growth Phases in Wee1-50 Mutant Cells
3.5. Analysis of Growth Phases in Pom1δ Mutant Cells
3.6. Analysis of Both Growth Segments in the Same Cells
4. Discussion
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Wild-Type | wee1-50 | pom1Δ | ||||
---|---|---|---|---|---|---|
N | % | N | % | N | % | |
Bilinear | 40 | 65.6 | 65 | 69.9 | 85 | 59.0 |
Linear | 15 | 24.6 | 22 | 23.7 | 47 | 32.6 |
Exponential | 6 | 9.8 | 6 | 6.4 | 12 | 8.3 |
Total | 61 | 100.0 | 93 | 100.0 | 144 | 100.0 |
WT | ||||||
---|---|---|---|---|---|---|
N | Mean ± SD (min) | |||||
Total | Exp | Lin | Exp | Lin | p | |
All growth phases | 23 | 57 | 37.4 ± 16.0 | 52.6 ± 20.7 | * 0.001 | |
Growth phase I | 17 | 23 | 31.6 ± 9.4 | 42.0 ± 16.5 | * 0.026 | |
Growth phase II | 6 | 34 | 54.0 ± 19.9 | 59.8 ± 20.3 | 0.520 | |
<40 | All growth phases | 16 | 14 | 29.2 ± 5.7 | 27.5 ± 7.0 | 0.468 |
Growth phase I | 15 | 11 | 29.1 ± 5.9 | 28.2 ± 7.2 | 0.740 | |
Growth phase II | 1 | 3 | 30.1 | 24.6 ± 6.1 | − | |
≥40 | All growth phases | 7 | 43 | 56.3 ± 15.9 | 60.8 ± 16.6 | 0.513 |
Growth phase I | 2 | 12 | 50.2 ± 11.4 | 54.5 ± 11.6 | 0.708 | |
Growth phase II | 5 | 31 | 58.8 ± 18.0 | 63.2 ± 17.8 | 0.628 |
wee1-50 | ||||||
---|---|---|---|---|---|---|
N | Mean ± SD (min) | |||||
Total | Exp | Lin | Exp | Lin | p | |
All growth phases | 39 | 91 | 45.7 ± 21.0 | 59.1 ± 28.0 | * 0.003 | |
Growth phase I | 21 | 44 | 47.6 ± 20.7 | 63.9 ± 30.6 | * 0.014 | |
Growth phase II | 18 | 47 | 43.4 ± 21.7 | 54.7 ± 24.7 | 0.080 | |
<40 | All growth phases | 18 | 31 | 29.0 ± 5.3 | 31.1 ± 6.0 | 0.200 |
Growth phase I | 9 | 13 | 28.7 ± 5.6 | 32.3 ± 5.5 | 0.149 | |
Growth phase II | 9 | 18 | 29.3 ± 5.3 | 30.3 ± 6.3 | 0.673 | |
≥40 | All growth phases | 21 | 60 | 59.9 ± 18.7 | 73.6 ± 23.4 | * 0.010 |
Growth phase I | 12 | 31 | 61.7 ± 15.7 | 77.1 ± 26.8 | * 0.026 | |
Growth phase II | 9 | 29 | 57.6 ± 22.9 | 69.9 ± 18.9 | 0.170 |
pom1Δ | ||||||
---|---|---|---|---|---|---|
N | Mean ± SD (min) | |||||
Total | Exp | Lin | Exp | Lin | p | |
All growth phases | 50 | 120 | 36.6 ± 14.2 | 45.8 ± 19.5 | * 0.001 | |
Growth phase I | 31 | 54 | 33.4 ± 12.9 | 36.8 ± 17.6 | 0.312 | |
Growth phase II | 19 | 66 | 41.7 ± 15.1 | 53.1 ± 17.9 | * 0.009 | |
<40 | All growth phases | 33 | 52 | 27.6 ± 6.1 | 27.6 ± 6.5 | 0.997 |
Growth phase I | 24 | 35 | 27.5 ± 6.1 | 26.2 ± 5.9 | 0.451 | |
Growth phase II | 9 | 17 | 28.0 ± 6.3 | 30.4 ± 7.0 | 0.382 | |
≥40 | All growth phases | 17 | 68 | 54.0 ± 7.6 | 59.7 ± 13.8 | * 0.027 |
Growth phase I | 7 | 19 | 53.7 ± 7.9 | 56.2 ± 15.4 | 0.602 | |
Growth phase II | 10 | 49 | 54.1 ± 7.8 | 61.0 ± 13.0 | * 0.038 |
WT | wee1-50 | pom1Δ | Σ = WT + wee1-50 + pom1Δ | |
---|---|---|---|---|
Lin-Lin | 9 | 15 | 8 | 32 (71%) |
Exp-Lin | 3 | 1 | 2 | 6 (13%) |
Lin-Exp | 0 | 3 | 1 | 4 (9%) |
Exp-Exp | 0 | 3 | 0 | 3 (7%) |
All | 12 | 22 | 11 | 45 (100%) |
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Pesti, B.; Nagy, Z.; Papp, L.A.; Sipiczki, M.; Sveiczer, Á. Cell Length Growth in the Fission Yeast Cell Cycle: Is It (Bi)linear or (Bi)exponential? Processes 2021, 9, 1533. https://doi.org/10.3390/pr9091533
Pesti B, Nagy Z, Papp LA, Sipiczki M, Sveiczer Á. Cell Length Growth in the Fission Yeast Cell Cycle: Is It (Bi)linear or (Bi)exponential? Processes. 2021; 9(9):1533. https://doi.org/10.3390/pr9091533
Chicago/Turabian StylePesti, Benedek, Zsófia Nagy, László Attila Papp, Matthias Sipiczki, and Ákos Sveiczer. 2021. "Cell Length Growth in the Fission Yeast Cell Cycle: Is It (Bi)linear or (Bi)exponential?" Processes 9, no. 9: 1533. https://doi.org/10.3390/pr9091533