A Novel Through-Thickness Perfusion Bioreactor for the Generation of Scaffold-Free Tissue Engineered Cartilage
Abstract
:1. Introduction
2. Experimental Section
2.1. Perfusion Bioreactor Design
2.2. Cell Isolation and Cell Culture
2.3. Biomechanics and Biochemistry
2.4. Histology
2.5. Modelling
3. Results and Discussion
3.1. Bioreactor Design
3.2. Cell Culture
Experiment | Media Flow | Construct Thickness (mm) | Young’s Modulus (kPa) | Hydroxyproline/DNA (μg/μg) | GAG/DNA (μg/μg) |
---|---|---|---|---|---|
Method 1 | Static | 0.79 (±0.056) | 46.6 (±2.4) | 0.0347 (±0.0033) | 0.1199 (±0.0272) |
Perfused | 3.05 (±0.36) * | 2.4 (±1.0) * | 0.0332 (±0.0010) | 0.1441 (±0.0836) | |
Method 2 | Static | n/a | 21.0 (±5.7) ** | 0.0353 (±0.0022) | 0.0398 (±0.0078) |
Perfused | 0.944 (±.287) | 15.8 (±12.1) | 0.0328 (±0.0045) | 0.04452 (±0.00454) | |
Method 3 | Static | 0.43 (±.302) | 37.6 (±12.7) | 0.301 (±0.040) | 100.10 (±24.6) |
Perfused | 0.524 (±.0622) | 86.6 (±23.9) * | 0.354 (±0.080) | 75.17 (±5.0) |
3.3. Modelling
3.4. Discussion
4. Conclusions
Author Contributions
Conflicts of Interest
References
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Gilbert, E.; Mosher, M.; Gottipati, A.; Elder, S. A Novel Through-Thickness Perfusion Bioreactor for the Generation of Scaffold-Free Tissue Engineered Cartilage. Processes 2014, 2, 658-674. https://doi.org/10.3390/pr2030658
Gilbert E, Mosher M, Gottipati A, Elder S. A Novel Through-Thickness Perfusion Bioreactor for the Generation of Scaffold-Free Tissue Engineered Cartilage. Processes. 2014; 2(3):658-674. https://doi.org/10.3390/pr2030658
Chicago/Turabian StyleGilbert, Eric, Mark Mosher, Anuhya Gottipati, and Steven Elder. 2014. "A Novel Through-Thickness Perfusion Bioreactor for the Generation of Scaffold-Free Tissue Engineered Cartilage" Processes 2, no. 3: 658-674. https://doi.org/10.3390/pr2030658