Separation of Molar Weight-Distributed Polyethylene Glycols by Reversed-Phase Chromatography—II. Preparative Isolation of Pure Single Homologs
Abstract
:1. Introduction
2. Theoretical Background
2.1. Thermodynamic Retention Model
2.2. Column Model Based on Discrete Convolution
2.3. Linear Solvent Strength Theory
3. Experimental
3.1. Materials
3.2. Preparative Chromatography
3.3. Analytical Chromatography
4. Results and Discussion
4.1. Separation Problem and Role of Operating Conditions
4.2. Thermodynamic Analysis
4.3. Separation under Linear Conditions
4.4. Separation under Nonlinear Conditions
4.4.1. Isocratic Operation
4.4.2. Gradient-Based Operation
5. Summary and Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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ACN | ||||||
---|---|---|---|---|---|---|
vol% | - | % | - | % | % | |
0 | 12.35 | 14,000 | 1.11 | |||
10 | 12.25 | −0.8 | 12,810 | −8.5 | 1.19 | 7.2 |
20 | 12.22 | −1.1 | 10,070 | −28.1 | 1.29 | 16.2 |
35 | 12.18 | −1.4 | 6170 | −55.9 | 1.36 | 22.5 |
50 | 12.20 | −1.2 | 6010 | −57.1 | 1.37 | 23.4 |
ACN | ||||
---|---|---|---|---|
vol% | kJ/mol | kJ/mol | J/(mol K) | J/(mol K) |
15 | 1.185 | −4.423 | 6.401 | −29.390 |
17 | 1.365 | −4.773 | 6.533 | −30.131 |
19 | 1.485 | −5.153 | 6.551 | −30.999 |
21 | 1.566 | −5.488 | 6.487 | −31.548 |
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Supper, M.; Jost, R.; Bornschein, B.; Kaspereit, M. Separation of Molar Weight-Distributed Polyethylene Glycols by Reversed-Phase Chromatography—II. Preparative Isolation of Pure Single Homologs. Processes 2023, 11, 946. https://doi.org/10.3390/pr11030946
Supper M, Jost R, Bornschein B, Kaspereit M. Separation of Molar Weight-Distributed Polyethylene Glycols by Reversed-Phase Chromatography—II. Preparative Isolation of Pure Single Homologs. Processes. 2023; 11(3):946. https://doi.org/10.3390/pr11030946
Chicago/Turabian StyleSupper, Malvina, Rosanna Jost, Benedikt Bornschein, and Malte Kaspereit. 2023. "Separation of Molar Weight-Distributed Polyethylene Glycols by Reversed-Phase Chromatography—II. Preparative Isolation of Pure Single Homologs" Processes 11, no. 3: 946. https://doi.org/10.3390/pr11030946